LOCUS       sequence-242038-        8093 bp    DNA     circular SYN 14-APR-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8093)
  TITLE     Karl Kramer plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BirA(R118G)-HA destination vector
FEATURES             Location/Qualifiers
     source          1..8093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        26..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..609
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..580
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     654..673
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        654..672
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    710..834
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(859..864,865..882,883..889)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             943..1602
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1009..1028)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1944..2249
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2128..2147
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2290..2414)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2455..2802,2803..2805,2806..3414)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3418..3444
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3475..3492)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3481..3705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3751..4179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3838..3857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4048..4069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4188..4208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4193..4522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4373..4508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4435..4454
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4589..5383
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4643..4662)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5253..5272
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5557..5678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5594..5613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5648..5667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5727..5743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5727..5743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5740..5762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5751..5767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5775..5781,5782..5799,5800..5805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5820..5841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5958..5975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6129..6717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6209..6228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6888..7679,7680..7748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7511..7530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7749..7853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7928..7947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
       61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      601 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
      661 actcactata gggagaccca agctggctag cgcttaaggc ctgttatcaa caagtttgta
      721 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      781 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
      841 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      901 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      961 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1021 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1081 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1141 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1201 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1261 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1321 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1381 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1441 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1501 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1561 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1621 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1681 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1741 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1801 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1861 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1921 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1981 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2041 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2101 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2161 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2221 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2281 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     2341 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2401 ttgtacaaag tggttgataa ccggtcgtac gtctccggat tcgaattcgg atccaaggac
     2461 aacaccgtgc ccctgaagct gatcgccctg ctggccaacg gcgagttcca ctctggcgag
     2521 cagctgggag agaccctggg aatgagcaga gccgccatca acaagcacat ccagacactg
     2581 agagactggg gagtggacgt gttcaccgtg cctggcaagg gctacagcct gcctgagcct
     2641 atccagctgc tgaacgccaa gcagatcctg ggacagctgg atggcggaag cgtggccgtg
     2701 ctgcctgtga tcgactccac caatcagtac ctgctggaca gaatcggaga gctgaagtcc
     2761 ggcgacgcct gcatcgccga gtaccagcag gctggcagag gaggcagagg acggaagtgg
     2821 ttcagcccat tcggagccaa cctgtacctg tccatgttct ggagactgga gcagggacct
     2881 gctgctgcca tcggactgag tctggtgatc ggaatcgtga tggccgaggt gctgagaaag
     2941 ctgggagccg acaaggtgag agtgaagtgg cctaatgacc tgtacctcca ggaccgcaag
     3001 ctggctggca tcctggtgga gctgacaggc aagacaggcg atgccgctca gatcgtgatc
     3061 ggagccggaa tcaacatggc catgagaaga gtggaggaga gcgtggtgaa ccagggctgg
     3121 atcaccctgc aggaggctgg catcaacctg gaccggaaca ccctggccgc catgctgatc
     3181 agagagctga gagccgctct ggagctgttc gagcaggagg gactggctcc ttacctgagc
     3241 agatgggaga agctggacaa cttcatcaac agacctgtga agctgatcat cggcgacaag
     3301 gaaatcttcg gcatctccag aggaatcgac aagcagggag ctctgctgct ggagcaggac
     3361 ggaatcatca agccctggat gggcggagaa atctccctga gaagcgcaga gaaggcttac
     3421 ccttacgatg taccggatta cgcataggcg gcctaagttt aaaccgctga tcagcctcga
     3481 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3541 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3601 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3661 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3721 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3961 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4141 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4201 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4261 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4321 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4381 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4441 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4501 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4561 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     4621 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4681 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4741 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     4801 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4861 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4921 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4981 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5041 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5101 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5161 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5221 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5281 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5341 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5401 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5461 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5521 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5581 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5641 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5701 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5761 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5821 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5881 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5941 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6001 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6061 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6721 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6781 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6841 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6901 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6961 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7021 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7081 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7141 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7201 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7261 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7321 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7381 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7441 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7501 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7561 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7621 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7681 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7741 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7801 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7861 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     7921 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     7981 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8041 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgc
//
