LOCUS       sequence-242375-       10622 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding red fluorescent 
            protein mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_mCherry
FEATURES             Location/Qualifiers
     source          1..10622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3916..4626
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4061..4079)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4329..4348
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4647..4834
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4702..4726)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4909..6265
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4909..5252
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5253..6062
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5320..5339)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6068..6265
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6360..6377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6360..6376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6369..6391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9271..10365
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgcttatggt gagcaagggc gaggaggata acatggccat catcaaggag
     3961 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     4021 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     4081 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     4141 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     4201 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     4261 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     4321 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     4381 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     4441 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     4501 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     4561 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     4621 aagtaaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     4681 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     4741 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     4801 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
     4861 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     4921 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     4981 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     5041 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     5101 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     5161 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     5221 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga
     5281 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     5341 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     5401 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     5461 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     5521 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag
     5581 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     5641 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     5701 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     5761 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     5821 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     5881 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     5941 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     6001 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     6061 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     6121 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     6181 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     6241 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtcga
     6301 cgaactagta taacttcgta tatggtatta tatacgaagt tatgcggccg cgcgaattca
     6361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6481 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     6541 acgcatctgt gcggtatttc acaccgcata ccatcgtccg gatcaatgaa gtagatgagt
     6601 ttactacctg tatatacttt tttttttgtc aaagtcactt ttatggcatt tccatgttga
     6661 aggattagcc acagcactaa attttcagcg tatgattgct tttaaatatt taattttcat
     6721 cgttttttaa ttaatattca aaacgattta atgccttcta catctgaagt caagctcata
     6781 gactcgggtt gggtagctaa aactacatct aacacgaagg ggtatagcct tcttaaagta
     6841 tactcatgta taaacgtttt gggttcatat ttttgggcca cgtggtttat aaaaattctt
     6901 aactacacca ctcggtatcc cgcacccgtc tacgtttcta cgatttcgag ctaatattga
     6961 gtaaagtgat tagcaaaaaa aataaaagta ctcgttgtcg gagatcaaga atttttctat
     7021 tatctcatct aaaccacttt ctaaaagcga aaaacaaaat cgtaatatgc agcttgaatg
     7081 ggcttccata gtttgaaaga aaaaccctag cagtactggc aagggagaca ttccttttac
     7141 ctggctaatc gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac
     7201 ggatatagtt catgacaata tttacattgg tccagccacc agcttgcatg atctccggta
     7261 ttgaaactcc agcgcgggcc atatctcgcg cggctccgac acgggcactg tgtccagacc
     7321 aggccaggta tctctgacca gagtcatcct tagcgccgta aatcaatcga tgagttgctt
     7381 caaaaatccc ttccagggcg cgagttgata gctggctggt ggcagatggc gcggcaacac
     7441 cattttttct gacccggcaa aacaggtagt tattcggatc atcagctaca ccagagacgg
     7501 aaatccatcg ctcgaccagt ttagttaccc ccaggctaag tgccttctct acacctgcgg
     7561 tgctaaccag cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg
     7621 agatatcttt aaccctgatc ctggcaattt cggctatacg taacagggtg ttataagcaa
     7681 tccccagaaa tgccagatta cgtatatcct ggcagcgatc gctattttcc atgagtgaac
     7741 gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg ttttgcacgt tcaccggcat
     7801 caacgttttc ttttcggatc cgccgcataa ccagtgaaac agcattgctg tcacttggtc
     7861 gtggcagccc ggaccgacga tgaagcatgt ttagctggcc caaatgttgc tggatagttt
     7921 ttactgccag accgcgcgcc tgaagatata gaagataatc gcgaacatct tcaggttctg
     7981 cgggaaacca tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca
     8041 gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc cctgaacatg tccatcaggt
     8101 tcttgcgaac ctcatcactc gttgcatcga ccggtaactt ttagattaac atttgtttaa
     8161 atatgaaatt aggcaatgta acatactgca ggcaaatttt ggtgtacggt aagtaaattg
     8221 gacatatgat ttaacaaagc gactataagt cagaaagtga gaatgagatt gaaataatta
     8281 attcaactta ttcaattgat gatatgccag gattcctctt cctttatctt caatgtttcg
     8341 attaacaggt ccattatccg gtttttagtt tcgctgcaca ttgccgaatg acaatcggat
     8401 tcctttaagc tttgcttttg ccctgttttc tctccccagt aatcgttggg tgactgaacc
     8461 atccagtagc cgagagttac gtttttcctg atttaataaa tatccatatc tatttgtctc
     8521 gtggtgtttt acagcaacta accgaaatta ctattttgca tcatccaacc ataaagagac
     8581 aacaataatt ctagaaaaca tataacaagt gtttttttct atttacgtct catacgcaaa
     8641 gtccacttta ccatgttttg gaaacttgta cccaaccatt catttttcga ttttgtaatg
     8701 aggctctttt cttctaacaa tcattgaggt cattatcatg tgtcctcacc tattgtttaa
     8761 attgaggctt gcgctattcc caaaattgtg ggtattattt tcatatatca gtatctgcaa
     8821 tttcggttca actccggaag ctcggtaccc aactgtggga atactcaggt atcgtaagat
     8881 gcaagagttc gaatctctta gcaaccatta ttttttttct ctcaacataa cgagaacaca
     8941 caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag
     9001 gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag
     9061 cgccggaacc ggcttttcat atagaataga gaagcgttca tgactaaatg cttgcatcac
     9121 aatacttgaa gttgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc
     9181 aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat atatatatat
     9241 atatatatat ttcaaggata taccattgta atgtctgccc ctaagaagat cgtcgttttg
     9301 ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt
     9361 tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct
     9421 atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa gaaggctgat
     9481 gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt tagacctgaa
     9541 caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac
     9601 tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact
     9661 gacttcgttg ttgttagaga attagtggga ggtatttact ttggtaagag aaaggaagac
     9721 gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc
     9781 acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg
     9841 gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc
     9901 aagaacgaat ttcctacatt gaaagttcaa catcaattga ttgattctgc cgccatgatc
     9961 ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat
    10021 atcatctccg atgaagcctc cgttatccca ggctccttgg gtttgttgcc atctgcgtcc
    10081 ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccatggttcc
    10141 gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg
    10201 atgttgaaat tgtcattgaa cttgcctgaa gaaggtaaag ccattgaaga tgcagttaaa
    10261 aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa
    10321 gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga ttctcttttt
    10381 ttgtgatatt tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta
    10441 caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc
    10501 aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc
    10561 aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcatca
    10621 ca
//
