LOCUS       sequence-242539-         705 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Constitutive, ubiquitous mCherry expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   McCauley KB, Hawkins F, Serra M, Thomas DC, Jacob A, Kotton DN
  TITLE     Efficient Derivation of Functional Human Airway Epithelium from 
            Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling.
  JOURNAL   Cell Stem Cell. 2017 Jun 1;20(6):844-857.e6. doi: 
            10.1016/j.stem.2017.03.001. Epub 2017 Mar 30.
  PUBMED    28366587
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2017.03.001"; journalName: "Cell Stem Cell"; date: 
            "2017-06-1- 1"; volume: "20"; issue: "6"; pages: "844-857.e6"
COMMENT     Sequence Label: pHAGE2-EF1aL-mCherry-W
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..705
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(143..161)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     411..430
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
       61 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      121 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      181 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      241 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      301 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      361 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      421 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
      481 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
      541 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
      601 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
      661 gccgagggcc gccactccac cggcggcatg gacgagctgt acaag
//
