LOCUS       sequence-242755-       10270 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fluorescent version of smFP FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Looger Lab
  TITLE     Looger Lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFCK_smFP FLAG _ Bright
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        509..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             725..905
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    952..1077
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1570..1803
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1988..2032
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2330..2447
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2492..3780
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3802..3811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3811..3834
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3838..3861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3865..3888
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4417..4440
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4447..4470
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4483..4506
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4513..4536
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4747..4770
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4774..4797
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4801..4824
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4852..5440
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4905..4925)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5323..5334)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5443..5459)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5444..5460)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5965..6145
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6171..6188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6177..6401
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6447..6875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6534..6553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6744..6765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6884..6904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6889..7218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7069..7204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7131..7150
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7266..7313
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7332..7706
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7836..7957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7873..7892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7927..7946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8006..8022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8006..8022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8019..8041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8030..8046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8054..8060,8061..8078,8079..8084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8099..8120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8237..8254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8408..8996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8488..8507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9167..9958,9959..10027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9790..9809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10028..10132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10207..10226)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
       61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
      721 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      781 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      841 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      901 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      961 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1021 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1081 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1141 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1201 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1261 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1321 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1381 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1441 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1501 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1561 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1621 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1681 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1741 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1801 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1861 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1921 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1981 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2041 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2101 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2161 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2221 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2281 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     2341 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2401 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2461 gggacagcag agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca
     2521 tggggttctt cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg
     2581 acctgcccag ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct
     2641 taaattccat gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg
     2701 cagatgacca atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc
     2761 ccagccctgg gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac
     2821 cttgacggga agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg
     2881 tccctctggg aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc
     2941 tcagtggtga cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc
     3001 tgtttggtac ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca
     3061 gggagagctg gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca
     3121 gaggaagcaa agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag
     3181 cttcccctgt aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct
     3241 tcagaggaga tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga
     3301 atgtggcacc cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc
     3361 cgtctgttaa aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt
     3421 gtggactaag tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa
     3481 cagggtccac ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg
     3541 caaagaggag caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg
     3601 caacgggaac agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct
     3661 cgcgaggctg tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc
     3721 ggttctccgt ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc
     3781 gggggatccc cgggtaccgg tgccaccatg gactacaagg acgacgacga taaaggagat
     3841 tacaaagatg acgatgataa gggcgattat aaggacgacg atgacaaggg cggcgtgtcg
     3901 aaaggcgagg agctgttcac aggcgtggtc cccatcctgg ttgagctgga cggtgacgtt
     3961 aacggccaca aatttagcgt acgtggtgag ggcgagggtg acgctaccaa cggaaagctg
     4021 accctgaaat tcatctgcac aactggcaag ctgccggtgc cctggcccac gctggtaacc
     4081 actctgacct atggcgttca atgtttcagt cgttacccgg atcacatgaa gcagcatgac
     4141 ttcttcaagt cggccatgcc cgaaggctac gtgcaggagc gcaccatcag ttttaaggat
     4201 gacggcacct acaagactcg cgccgaggtc aagttcgaag gagacaccct ggttaatcgt
     4261 atcgagctca aaggcatcga tttcaaggag gacggaaata ttctgggcca caagctggag
     4321 tacaatttta acagccacaa tgtttacatt actgccgaca agcagaagaa cggcatcaag
     4381 gctaatttca agattcgtca caatgtggag ggaggcgact ataaggatga cgatgacaaa
     4441 caacaagact ataaggacga tgatgacaaa ggtcagcaag gcgattacaa ggacgatgac
     4501 gataagcagc aagattacaa ggatgatgat gataagggag gcgacggaag cgtgcagctg
     4561 gcagatcatt accaacagaa cacgcccatt ggtgacggtc cagtcctgct gcctgataac
     4621 cactacttgt ccacccagtc ggtgctgtcg aaggacccga acgagaagcg tgaccacatg
     4681 gtgctgctgg agttcgtgac cgccgcagga atcaccctcg gcatggatga gctttacaag
     4741 ggcggagatt ataaagatga cgacgacaag ggcgactaca aagatgatga tgacaagggt
     4801 gactacaaag acgacgatga taaataagaa ttcgatatca agcttatcga taatcaacct
     4861 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4921 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4981 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5041 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5101 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5161 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5221 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5281 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5341 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5401 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5461 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5521 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5581 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5641 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     5701 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     5761 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     5821 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     5881 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     5941 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     6001 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6061 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6121 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     6181 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6241 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6301 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6361 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6421 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6481 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6541 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6601 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6661 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6721 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6781 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6841 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6901 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6961 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7021 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7141 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7201 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     7261 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7321 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7381 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7441 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7501 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7561 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7621 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7681 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     7741 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7801 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7981 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10141 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    10201 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10261 gttggaggtc
//
