LOCUS       sequence-243191-       11798 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of PIK3CG E347K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CG-E347K
FEATURES             Location/Qualifiers
     source          1..11798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6354..6365
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(6758..6782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6790..6808
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6833..6852)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6834..6852)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6858..6878)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6932..6973
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6994..7561
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7011..7563
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7155..7172)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7382..7401
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7575..8174
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7575..7594)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8071..8091
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8188..8776
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8241..8261)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8659..8670)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8851..9084
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9203..9221)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9289..9393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9394..9462,9463..10254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9612..9631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10425..11013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10914..10933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11167..11184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11424..11445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11460..11465,11466..11483,11484..11490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11498..11514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11503..11525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11522..11538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11522..11538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11588..11607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagctggaga actataaaca gcccgtggtg
     3481 ctgagagagg acaactgccg aaggcgccgg aggatgaagc cgcgcagtgc tgcggccagc
     3541 ctgtcctcca tggagctcat ccccatcgag ttcgtgctgc ccaccagcca gcgcaaatgc
     3601 aagagccccg aaacggcgct gctgcacgtg gccggccacg gcaacgtgga gcagatgaag
     3661 gcccaggtgt ggctgcgagc gctggagacc agcgtggcgg cggacttcta ccaccggctg
     3721 ggaccgcatc acttcctcct gctctatcag aagaaggggc agtggtacga gatctacgac
     3781 aagtaccagg tggtgcagac tctggactgc ctgcgctact ggaaggccac gcaccggagc
     3841 ccgggccaga tccacctggt gcagcggcac ccgccctccg aggagtccca agccttccag
     3901 cggcagctca cggcgctgat tggctatgac gtcactgacg tcagcaacgt gcacgacgat
     3961 gagctggagt tcacgcgccg tggcttggtg accccgcgca tggcggaggt ggccagccgc
     4021 gaccccaagc tctacgccat gcacccgtgg gtgacgtcca agcccctccc ggagtacctg
     4081 tggaagaaga ttgccaacaa ctgcatcttc atcgtcattc accgcagcac caccagccag
     4141 accattaagg tctcacccga cgacaccccc ggcgccatcc tgcagagctt cttcaccaag
     4201 atggccaaga agaaatctct gatggatatt cccgaaagcc aaagcgaaca ggattttgtg
     4261 ctgcgcgtct gtggccggga tgagtacctg gtgggcgaaa cgcccatcaa aaacttccag
     4321 tgggtgaggc actgcctcaa gaacggagaa gagattcacg tggtactgga cacgcctcca
     4381 gacccggccc tagacgaggt gaggaaggaa gagtggccgc tggtggatga ctgcacggga
     4441 gtcaccggct accatgagca gcttaccatc cacggcaagg accacaagag tgtgttcacc
     4501 gtgtccctgt gggactgcga ccgcaagttc agggtcaaga tcagaggcat tgatatcccc
     4561 gtcctgcctc ggaacaccga cctcacagtt tttgtagagg caaacatcca gcatgggcaa
     4621 caagtccttt gccaaaggag aaccagcccc aaacccttca cagaggaggt gctgtggaat
     4681 gtgtggcttg agttcagtat caaaatcaaa gacttgccca aaggggctct actgaacctc
     4741 cagatctact gcggtaaagc tccagcactg tccagcaagg cctctgcaga gtcccccagt
     4801 tctgagtcca agggcaaagt tcagcttctc tattatgtga acctgctgct gatagaccac
     4861 cgtttcctcc tgcgccgtgg agaatacgtc ctccacatgt ggcagatatc tgggaaggga
     4921 gaagaccaag gaagcttcaa tgctgacaaa ctcacgtctg caactaaccc agacaaggag
     4981 aactcaatgt ccatctccat tcttctggac aattactgcc acccgatagc cctgcctaag
     5041 catcagccca cccctgaccc ggaaggggac cgggttcgag cagaaatgcc caaccagctt
     5101 cgcaagcaat tggaggcgat catagccact gatccactta accctctcac agcagaggac
     5161 aaagaattgc tctggcattt tagatacgaa agccttaagc acccaaaagc atatcctaag
     5221 ctatttagtt cagtgaaatg gggacagcaa gaaattgtgg ccaaaacata ccaattgttg
     5281 gccagaaggg aagtctggga tcaaagtgct ttggatgttg ggttaacaat gcagctcctg
     5341 gactgcaact tctcagatga aaatgtaaga gccattgcag ttcagaaact ggagagcttg
     5401 gaggacgatg atgttctgca ttaccttcta caattggtcc aggctgtgaa atttgaacca
     5461 taccatgata gcgcccttgc cagatttctg ctgaagcgtg gtttaagaaa caaaagaatt
     5521 ggtcactttt tgttttggtt cttgagaagt gagatagccc agtccagaca ctatcagcag
     5581 aggttcgctg tgattctgga agcctatctg aggggctgtg gcacagccat gctgcacgac
     5641 tttacccaac aagtccaagt aatcgagatg ttacaaaaag tcacccttga tattaaatcg
     5701 ctctctgctg aaaagtatga cgtcagttcc caagttattt cacaacttaa acaaaagctt
     5761 gaaaacctgc agaattctca actccccgaa agctttagag ttccatatga tcctggactg
     5821 aaagcaggag cgctggcaat tgaaaaatgt aaagtaatgg cctccaagaa aaaaccacta
     5881 tggcttgagt ttaaatgtgc cgatcctaca gccctatcaa atgaaacaat tggaattatc
     5941 tttaaacatg gtgatgatct gcgccaagac atgcttattt tacagattct acgaatcatg
     6001 gagtctattt gggagactga atctttggat ctatgcctcc tgccatatgg ttgcatttca
     6061 actggtgaca aaataggaat gatcgagatt gtgaaagacg ccacgacaat tgccaaaatt
     6121 cagcaaagca cagtgggcaa cacgggagca tttaaagatg aagtcctgaa tcactggctc
     6181 aaagaaaaat cccctactga agaaaagttt caggcagcag tggagagatt tgtttattcc
     6241 tgtgcaggct actgtgtggc aacctttgtt cttggaatag gcgacagaca caatgacaat
     6301 attatgatca ccgagacagg aaacctattt catattgact tcgggcacat tcttgggaat
     6361 tacaaaagtt tcctgggcat taataaagag agagtgccat ttgtgctaac ccctgacttc
     6421 ctctttgtga tgggaacttc tggaaagaag acaagcccac acttccagaa atttcaggac
     6481 atctgtgtta aggcttatct agcccttcgt catcacacaa acctactgat catcctgttc
     6541 tccatgatgc tgatgacagg aatgccccag ttaacaagca aagaagacat tgaatatatc
     6601 cgggatgccc tcacagtggg gaaaaatgag gaggatgcta aaaagtattt tcttgatcag
     6661 atcgaagttt gcagagacaa aggatggact gtgcagttta attggtttct acatcttgtt
     6721 cttggcatca aacaaggaga gaaacattca gcctagaacc cagctttctt gtacaaagtg
     6781 gtgatatcca attaaccctc actaaaggat gtaattgtga ataatgtatg tgccctatag
     6841 tgagtcgtat taccacccaa cttttctata caaagtggtt gatatccagc acagtggcgg
     6901 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     6961 tctcgattct acgcgtaccg gttagtaatg agatccctcc cccccccnta acgttactgg
     7021 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7081 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7141 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     7201 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     7261 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     7321 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     7381 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     7441 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     7501 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     7561 atatggccac acatatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7621 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7681 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7741 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7801 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7861 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7921 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7981 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     8041 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     8101 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     8161 agcccggtgc ctaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     8221 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8281 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8341 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8401 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8461 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8521 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8581 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8641 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8701 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8761 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     8821 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8881 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9061 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     9121 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     9181 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9241 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9601 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11221 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    11281 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    11341 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11521 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    11581 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    11641 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    11701 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11761 aattgtattt gttaaatatg tactacaaac ttagtagt
//
