LOCUS       sequence-243855-       13893 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses multimeric human VWF with C-terminal c-Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pcDNA3.1-WT-VWF
FEATURES             Location/Qualifiers
     source          1..13893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     702..721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        702..720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2384..2395
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             9264..9293
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(9321..9338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9327..9551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9597..10025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9684..9703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9894..9915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10034..10054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10039..10368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10219..10354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10281..10300
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10435..11229
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10489..10508)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11099..11118
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11405..11526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11442..11461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11496..11515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11575..11591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11575..11591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11588..11610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11599..11615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11623..11629,11630..11647,11648..11653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11668..11689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11806..11823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11977..12565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12057..12076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12736..13527,13528..13596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13359..13378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13597..13701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13776..13795)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      661 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      721 gagacccaag ctggctagtt aagcttggta ccgagctcgg atccactagt ccagtgtggt
      781 ggaattcccg cagccctcat ttgcagggga agatgattcc tgccagattt gccggggtgc
      841 tgcttgctct ggccctcatt ttgccaggga ccctttgtgc agaaggaact cgcggcaggt
      901 catccacggc ccgatgcagc cttttcggaa gtgacttcgt caacaccttt gatgggagca
      961 tgtacagctt tgcgggatac tgcagttacc tcctggcagg gggctgccag aaacgctcct
     1021 tctcgattat tggggacttc cagaatggca agagagtgag cctctccgtg tatcttgggg
     1081 aattttttga catccatttg tttgtcaatg gtaccgtgac acagggggac caaagagtct
     1141 ccatgcccta tgcctccaaa gggctgtatc tagaaactga ggctgggtac tacaagctgt
     1201 ccggtgaggc ctatggcttt gtggccagga tcgatggcag cggcaacttt caagtcctgc
     1261 tgtcagacag atacttcaac aagacctgcg ggctgtgtgg caactttaac atctttgctg
     1321 aagatgactt tatgacccaa gaagggacct tgacctcgga cccttatgac tttgccaact
     1381 catgggctct gagcagtgga gaacagtggt gtgaacgggc atctcctccc agcagctcat
     1441 gcaacatctc ctctggggaa atgcagaagg gcctgtggga gcagtgccag cttctgaaga
     1501 gcacctcggt gtttgcccgc tgccaccctc tggtggaccc cgagcctttt gtggccctgt
     1561 gtgagaagac tttgtgtgag tgtgctgggg ggctggagtg cgcctgccct gccctcctgg
     1621 agtacgcccg gacctgtgcc caggagggaa tggtgctgta cggctggacc gaccacagcg
     1681 cgtgcagccc agtgtgccct gctggtatgg agtataggca gtgtgtgtcc ccttgcgcca
     1741 ggacctgcca gagcctgcac atcaatgaaa tgtgtcagga gcgatgcgtg gatggctgca
     1801 gctgccctga gggacagctc ctggatgaag gcctctgcgt ggagagcacc gagtgtccct
     1861 gcgtgcattc cggaaagcgc taccctcccg gcacctccct ctctcgagac tgcaacacct
     1921 gcatttgccg aaacagccag tggatctgca gcaatgaaga atgtccaggg gagtgccttg
     1981 tcacaggtca atcacacttc aagagctttg acaacagata cttcaccttc agtgggatct
     2041 gccagtacct gctggcccgg gattgccagg accactcctt ctccattgtc attgagactg
     2101 tccagtgtgc tgatgaccgc gacgctgtgt gcacccgctc cgtcaccgtc cggctgcctg
     2161 gcctgcacaa cagccttgtg aaactgaagc atggggcagg agttgccatg gatggccagg
     2221 acgtccagct ccccctcctg aaaggtgacc tccgcatcca gcatacagtg acggcctccg
     2281 tgcgcctcag ctacggggag gacctgcaga tggactggga tggccgcggg aggctgctgg
     2341 tgaagctgtc ccccgtctat gccgggaaga cctgcggcct gtgtgggaat tacaatggca
     2401 accagggcga cgacttcctt accccctctg ggctggcgga gccccgggtg gaggacttcg
     2461 ggaacgcctg gaagctgcac ggggactgcc aggacctgca gaagcagcac agcgatccct
     2521 gcgccctcaa cccgcgcatg accaggttct ccgaggaggc gtgcgcggtc ctgacgtccc
     2581 ccacattcga ggcctgccat cgtgccgtca gcccgctgcc ctacctgcgg aactgccgct
     2641 acgacgtgtg ctcctgctcg gacggccgcg agtgcctgtg cggcgccctg gccagctatg
     2701 ccgcggcctg cgcggggaga ggcgtgcgcg tcgcgtggcg cgagccaggc cgctgtgagc
     2761 tgaactgccc gaaaggccag gtgtacctgc agtgcgggac cccctgcaac ctgacctgcc
     2821 gctctctctc ttacccggat gaggaatgca atgaggcctg cctggagggc tgcttctgcc
     2881 ccccagggct ctacatggat gagagggggg actgcgtgcc caaggcccag tgcccctgtt
     2941 actatgacgg tgagatcttc cagccagaag acatcttctc agaccatcac accatgtgct
     3001 actgtgagga tggcttcatg cactgtacca tgagtggagt ccccggaagc ttgctgcctg
     3061 acgctgtcct cagcagtccc ctgtctcatc gcagcaaaag gagcctatcc tgtcggcccc
     3121 ccatggtcaa gctggtgtgt cccgctgaca acctgcgggc tgaagggctc gagtgtacca
     3181 aaacgtgcca gaactatgac ctggagtgca tgagcatggg ctgtgtctct ggctgcctct
     3241 gccccccggg catggtccgg catgagaaca gatgtgtggc cctggaaagg tgtccctgct
     3301 tccatcaggg caaggagtat gcccctggag aaacagtgaa gattggctgc aacacttgtg
     3361 tctgtcggga ccggaagtgg aactgcacag accatgtgtg tgatgccacg tgctccacga
     3421 tcggcatggc ccactacctc accttcgacg ggctcaaata cctgttcccc ggggagtgcc
     3481 agtacgttct ggtgcaggat tactgcggca gtaaccctgg gacctttcgg atcctagtgg
     3541 ggaataaggg atgcagccac ccctcagtga aatgcaagaa acgggtcacc atcctggtgg
     3601 agggaggaga gattgagctg tttgacgggg aggtgaatgt gaagaggccc atgaaggatg
     3661 agactcactt tgaggtggtg gagtctggcc ggtacatcat tctgctgctg ggcaaagccc
     3721 tctccgtggt ctgggaccgc cacctgagca tctccgtggt cctgaagcag acataccagg
     3781 agaaagtgtg tggcctgtgt gggaattttg atggcatcca gaacaatgac ctcaccagca
     3841 gcaacctcca agtggaggaa gaccctgtgg actttgggaa ctcctggaaa gtgagctcgc
     3901 agtgtgctga caccagaaaa gtgcctctgg actcatcccc tgccacctgc cataacaaca
     3961 tcatgaagca gacgatggtg gattcctcct gtagaatcct taccagtgac gtcttccagg
     4021 actgcaacaa gctggtggac cccgagccat atctggatgt ctgcatttac gacacctgct
     4081 cctgtgagtc cattggggac tgcgcctgct tctgcgacac cattgctgcc tatgcccacg
     4141 tgtgtgccca gcatggcaag gtggtgacct ggaggacggc cacattgtgc ccccagagct
     4201 gcgaggagag gaatctccgg gagaacgggt atgagtgtga gtggcgctat aacagctgtg
     4261 cacctgcctg tcaagtcacg tgtcagcacc ctgagccact ggcctgccct gtgcagtgtg
     4321 tggagggctg ccatgcccac tgccctccag ggaaaatcct ggatgagctt ttgcagacct
     4381 gcgttgaccc tgaagactgt ccagtgtgtg aggtggctgg ccggcgtttt gcctcaggaa
     4441 agaaagtcac cttgaatccc agtgaccctg agcactgcca gatttgccac tgtgatgttg
     4501 tcaacctcac ctgtgaagcc tgccaggagc cgggaggcct ggtggtgcct cccacagatg
     4561 ccccggtgag ccccaccact ctgtatgtgg aggacatctc ggaaccgccg ttgcacgatt
     4621 tctactgcag caggctactg gacctggtct tcctgctgga tggctcctcc aggctgtccg
     4681 aggctgagtt tgaagtgctg aaggcctttg tggtggacat gatggagcgg ctgcgcatct
     4741 cccagaagtg ggtccgcgtg gccgtggtgg agtaccacga cggctcccac gcctacatcg
     4801 ggctcaagga ccggaagcga ccgtcagagc tgcggcgcat tgccagccag gtgaagtatg
     4861 cgggcagcca ggtggcctcc accagcgagg tcttgaaata cacactgttc caaatcttca
     4921 gcaagatcga ccgccctgaa gcctcccgca tcgccctgct cctgatggcc agccaggagc
     4981 cccaacggat gtcccggaac tttgtccgct acgtccaggg cctgaagaag aagaaggtca
     5041 ttgtgatccc ggtgggcatt gggccccatg ccaacctcaa gcagatccgc ctcatcgaga
     5101 agcaggcccc tgagaacaag gccttcgtgc tgagcagtgt ggatgagctg gagcagcaaa
     5161 gggacgagat cgttagctac ctctgtgacc ttgcccctga agcccctcct cctactctgc
     5221 ccccccacat ggcacaagtc actgtgggcc cggggctctt gggggtttcg accctggggc
     5281 ccaagaggaa ctccatggtt ctggatgtgg cgttcgtcct ggaaggatcg gacaaaattg
     5341 gtgaagccga cttcaacagg agcaaggagt tcatggagga ggtgattcag cggatggatg
     5401 tgggccagga cagcatccac gtcacggtgc tgcagtactc ctacatggtg accgtggagt
     5461 accccttcag cgaggcacag tccaaagggg acatcctgca gcgggtgcga gagatccgct
     5521 accagggcgg caacaggacc aacactgggc tggccctgcg gtacctctct gaccacagct
     5581 tcttggtcag ccagggtgac cgggagcagg cgcccaacct ggtctacatg gtcaccggaa
     5641 atcctgcctc tgatgagatc aagaggctgc ctggagacat ccaggtggtg cccattggag
     5701 tgggccctaa tgccaacgtg caggagctgg agaggattgg ctggcccaat gcccctatcc
     5761 tcatccagga ctttgagacg ctcccccgag aggctcctga cctggtgctg cagaggtgct
     5821 gctccggaga ggggctgcag atccccaccc tctcccctgc acctgactgc agccagcccc
     5881 tggacgtgat ccttctcctg gatggctcct ccagtttccc agcttcttat tttgatgaaa
     5941 tgaagagttt cgccaaggct ttcatttcaa aagccaatat agggcctcgt ctcactcagg
     6001 tgtcagtgct gcagtatgga agcatcacca ccattgacgt gccatggaac gtggtcccgg
     6061 agaaagccca tttgctgagc cttgtggacg tcatgcagcg ggagggaggc cccagccaaa
     6121 tcggggatgc cttgggcttt gctgtgcgat acttgacttc agaaatgcat ggtgccaggc
     6181 cgggagcctc aaaggcggtg gtcatcctgg tcacggacgt ctctgtggat tcagtggatg
     6241 cagcagctga tgccgccagg tccaacagag tgacagtgtt ccctattgga attggagatc
     6301 gctacgatgc agcccagcta cggatcttgg caggcccagc aggcgactcc aacgtggtga
     6361 agctccagcg aatcgaagac ctccctacca tggtcacctt gggcaattcc ttcctccaca
     6421 aactgtgctc tggatttgtt aggatttgca tggatgagga tgggaatgag aagaggcccg
     6481 gggacgtctg gaccttgcca gaccagtgcc acaccgtgac ttgccagcca gatggccaga
     6541 ccttgctgaa gagtcatcgg gtcaactgtg accgggggct gaggccttcg tgccctaaca
     6601 gccagtcccc tgttaaagtg gaagagacct gtggctgccg ctggacctgc ccctgcgtgt
     6661 gcacaggcag ctccactcgg cacatcgtga cctttgatgg gcagaatttc aagctgactg
     6721 gcagctgttc ttatgtccta tttcaaaaca aggagcagga cctggaggtg attctccata
     6781 atggtgcctg cagccctgga gcaaggcagg gctgcatgaa atccatcgag gtgaagcaca
     6841 gtgccctctc cgtcgagctg cacagtgaca tggaggtgac ggtgaatggg agactggtct
     6901 ctgttcctta cgtgggtggg aacatggaag tcaacgttta tggtgccatc atgcatgagg
     6961 tcagattcaa tcaccttggt cacatcttca cattcactcc acaaaacaat gagttccaac
     7021 tgcagctcag ccccaagact tttgcttcaa agacgtatgg tctgtgtggg atctgtgatg
     7081 agaacggagc caatgacttc atgctgaggg atggcacagt caccacagac tggaaaacac
     7141 ttgttcagga atggactgtg cagcggccag ggcagacgtg ccagcccatc ctggaggagc
     7201 agtgtcttgt ccccgacagc tcccactgcc aggtcctcct cttaccactg tttgctgaat
     7261 gccacaaggt cctggctcca gccacattct atgccatctg ccagcaggac agttgccacc
     7321 aggagcaagt gtgtgaggtg atcgcctctt atgcccacct ctgtcggacc aacggggtct
     7381 gcgttgactg gaggacacct gatttctgtg ctatgtcatg cccaccatct ctggtctaca
     7441 accactgtga gcatggctgt ccccggcact gtgatggcaa cgtgagctcc tgtggggacc
     7501 atccctccga aggctgtttc tgccctccag ataaagtcat gttggaaggc agctgtgtcc
     7561 ctgaagaggc ctgcactcag tgcattggtg aggatggagt ccagcaccag ttcctggaag
     7621 cctgggtccc ggaccaccag ccctgtcaga tctgcacatg cctcagcggg cggaaggtca
     7681 actgcacaac gcagccctgc cccacggcca aagctcccac gtgtggcctg tgtgaagtag
     7741 cccgcctccg ccagaatgca gaccagtgct gccccgagta tgagtgtgtg tgtgacccag
     7801 tgagctgtga cctgccccca gtgcctcact gtgaacgtgg cctccagccc acactgacca
     7861 accctggcga gtgcagaccc aacttcacct gcgcctgcag gaaggaggag tgcaaaagag
     7921 tgtccccacc ctcctgcccc ccgcaccgtt tgcccaccct tcggaagacc cagtgctgtg
     7981 atgagtatga gtgtgcctgc aactgtgtca actccacagt gagctgtccc cttgggtact
     8041 tggcctcaac cgccaccaat gactgtggct gtaccacaac cacctgcctt cccgacaagg
     8101 tgtgtgtcca ccgaagcacc atctaccctg tgggccagtt ctgggaggag ggctgcgatg
     8161 tgtgcacctg caccgacatg gaggatgccg tgatgggcct ccgcgtggcc cagtgctccc
     8221 agaagccctg tgaggacagc tgtcggtcgg gcttcactta cgttctgcat gaaggcgagt
     8281 gctgtggaag gtgcctgcca tctgcctgtg aggtggtgac tggctcaccg cggggggact
     8341 cccagtcttc ctggaagagt gtcggctccc agtgggcctc cccggagaac ccctgcctca
     8401 tcaatgagtg tgtccgagtg aaggaggagg tctttataca acaaaggaac gtctcctgcc
     8461 cccagctgga ggtccctgtc tgcccctcgg gctttcagct gagctgtaag acctcagcgt
     8521 gctgcccaag ctgtcgctgt gagcgcatgg aggcctgcat gctcaatggc actgtcattg
     8581 ggcccgggaa gactgtgatg atcgatgtgt gcacgacctg ccgctgcatg gtgcaggtgg
     8641 gggtcatctc tggattcaag ctggagtgca ggaagaccac ctgcaacccc tgccccctgg
     8701 gttacaagga agaaaataac acaggtgaat gttgtgggag atgtttgcct acggcttgca
     8761 ccattcagct aagaggagga cagatcatga cactgaagcg tgatgagacg ctccaggatg
     8821 gctgtgatac tcacttctgc aaggtcaatg agagaggaga gtacttctgg gagaagaggg
     8881 tcacaggctg cccacccttt gatgaacaca agtgtctggc tgagggaggt aaaattatga
     8941 aaattccagg cacctgctgt gacacatgtg aggagcctga gtgcaacgac atcactgcca
     9001 ggctgcagta tgtcaaggtg ggaagctgta agtctgaagt agaggtggat attcactact
     9061 gccagggcaa atgtgccagc aaagccatgt actccattga catcaacgat gtgcaggacc
     9121 agtgctcctg ctgctctccg acacggacgg agcccatgca ggtggccctg cactgcacca
     9181 atggctctgt tgtgtaccat gaggttctca atgccatgga gtgcaaatgc tcccccagga
     9241 agtgcagcaa ggatatcgct agcgaacaga agctaataag tgaagaggac ctaatctgag
     9301 aattcaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     9361 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     9421 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     9481 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9541 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     9601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     9661 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9721 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9781 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9841 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9901 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9961 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10021 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    10081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    10141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    10201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    10261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    10321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    10381 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
    10441 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    10501 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
    10561 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
    10621 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    10681 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    10741 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    10801 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    10861 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    10921 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
    10981 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    11041 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    11101 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    11161 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    11221 ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc
    11281 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    11341 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    11401 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11521 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    11581 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    11641 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    11701 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    11761 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    11821 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11881 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    12001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    12061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    12121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    12181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12721 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12781 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12841 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12901 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12961 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    13021 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    13081 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13141 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13201 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13261 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13321 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13381 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13441 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13561 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13621 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13681 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    13741 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    13801 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    13861 atttaagcta caacaaggca aggcttgacc gac
//
