LOCUS       sequence-243865-       11105 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Tet3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Wang Y, Zhang Y
  TITLE     Regulation of TET protein stability by calpains.
  JOURNAL   Cell Rep. 2014 Jan 30;6(2):278-84. doi: 
            10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9.
  PUBMED    24412366
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.12.031"; journalName: "Cell Rep"; date: 
            "2014-01-30- 30"; volume: "6"; issue: "2"; pages: "278-84"
COMMENT     Sequence Label: pcDNA-Flag-Tet3
FEATURES             Location/Qualifiers
     source          1..11105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..934
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6609..6638
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6654..6671
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6694..6711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6700..6924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6970..7398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7057..7076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7267..7288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7407..7427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7412..7741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7592..7727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7654..7673
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7808..8602
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7862..7881)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8472..8491
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8778..8899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8815..8834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8869..8888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8948..8964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8948..8964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8961..8983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8972..8988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8996..9002,9003..9020,9021..9026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9041..9062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9179..9196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9350..9938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9430..9449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10109..10900,10901..10969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10732..10751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10970..11074)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttatg gactacaaag acgatgacga caagggatcc agccagtttc aggtgcccct
      961 ggccgtccag ccggacctgc caggccttta tgacttccct cagcgccagg tgatggtagg
     1021 gagcttcccg gggtctgggc tctccatggc tgggagtgag tcccaactcc gagggggtgg
     1081 agatggtcga aagaaacgga aacggtgtgg tacttgtgag ccctgccggc ggctggaaaa
     1141 ctgtggcgct tgcactagct gtaccaaccg ccgcacgcac cagatctgca aactgcgaaa
     1201 atgtgaggtg ctgaagaaaa aagtagggct tctcaaggag gtggaaataa aggctggtga
     1261 aggagccggg ccgtggggac aaggagcggc tgtcaagaca ggctcagagc tcagcccagt
     1321 tgatggacct gttccaggtc agatggactc agggccagtg taccatggag attcaaggca
     1381 gctaagcacc tcaggggcgc cggtcaatgg tgctagagag cccgccggac ccggtcttct
     1441 gggagctgcg ggtccttggc gggtagacca gaagcccgac tgggaggctg cctcaggccc
     1501 cactcacgct gctcgtctgg aagatgccca cgacctggtg gccttttcgg ccgtggccga
     1561 agctgtgtca tcttacgggg cccttagtac ccggctctat gaaaccttca accgtgagat
     1621 gagtcgtgag gctgggagca acggcagggg cccccggcct gagagctgct ctgagggcag
     1681 tgaagacctg gacacgctgc agacagccct ggcccttgca aggcatggca tgaaaccacc
     1741 caactgcacc tgcgatggcc cagagtgccc cgacttcctc gagtggctgg agggcaagat
     1801 caagtctatg gccatggagg gagggcaggg gcggcctagg ctcccaggcg ctctgcctcc
     1861 cagtgaggct ggcctcccag cccctagcac cagaccgcca ctccttagct ctgaggtccc
     1921 ccaggtacct cccctggagg gcctgcctct gtcccagagc gcgctgagca ttgccaagga
     1981 aaaaaacatc agcctgcaga cagccatcgc catcgaggcc ctcacacagc tctcctccgc
     2041 cctccctcag ccttctcatt ccacctccca ggcttcttgt ccactccctg aggccttgtc
     2101 cccttctgcc cctttcaggt ctccccagtc ctacctccgg gccccctcat ggcctgtggt
     2161 tcccccagag gaacatccat cctttgctcc tgacagccca gccttccctc cagcaacccc
     2221 aagacctgag ttttctgaag cgtggggcac tgacaccccc ccagcgacac cccggaactc
     2281 ctggcctgta cctcgcccaa gccctgaccc tatggcagaa ctggagcagc tattgggcag
     2341 cgccagtgat tacatccagt cagtattcaa gcggcctgag gccctgccca ccaagcccaa
     2401 ggtcaaggtt gaggccccct cttcttcccc tgctccggta ccatctccta tttctcagag
     2461 ggaggctccc ctgctgtctt cagagcctga cacccaccag aaggcccaga cagcccttca
     2521 gcaacatctt catcacaagc gcaacctatt cttggaacag gcccaagatg cctccttccc
     2581 tacttccaca gagcctcagg ctcctggttg gtgggcccct cccggctcac ctgccccaag
     2641 gcctcctgac aaaccaccca aggaaaagaa aaagaagccc cccacccctg ctggaggtcc
     2701 cgtgggagca gagaaaacca cccctgggat caagaccagt gtccgaaagc ccattcagat
     2761 caagaaatcc aggtccaggg acatgcagcc cctcttcctg cctgttaggc agattgttct
     2821 ggaagggcta aaaccccaag cctcagaagg acaggcaccg ttacccgccc agctctctgt
     2881 cccacctcct gcctcccagg gtgctgcatc ccagagctgt gccacccctc taaccccaga
     2941 accttctctt gcgctatttg cacctagtcc ctccggggac agcctgctgc cccctactca
     3001 ggaaatgaga tcccccagcc ccatggtagc cctgcagtca ggctccactg gtggccccct
     3061 tccccctgcc gatgacaagc tggaggagct catccggcaa tttgaggctg aatttgggga
     3121 tagctttggg cttcccggcc caccttcggt gcccattcaa gaacctgaaa accaatcaac
     3181 atgtctccca gctccggaga gcccttttgc cacccgctcc cccaagaaga tcaagatcga
     3241 gtcctcaggg gccgtgactg tgctctcaac tacctgcttc cattcagaag aggggggaca
     3301 ggaggccacg cccaccaagg ctgagaaccc actcacacca accctcagtg gcttcttgga
     3361 gtcacctcta aagtacctag acacacctac taagagtctg ctggacacac cggccaagaa
     3421 ggctcagtcc gagttcccta cctgcgattg tgtcgaacaa atagtggaga aagatgaagg
     3481 cccatattac actcacctgg gatctggccc cacagtagct tctatccggg aactcatgga
     3541 ggatcggtat ggagaaaagg ggaaagctat ccggattgag aaggtcatct acacgggcaa
     3601 ggaggggaag agttctcgag gctgtcccat cgccaagtgg gtgatccgaa gacacacact
     3661 ggaggagaag ctgctgtgcc tggtgcggca tcgggcaggc caccattgtc agaacgccgt
     3721 gattgttatc ttgatcctgg cctgggaggg catccctcga agccttgggg acaccctcta
     3781 ccaggagctt actgataccc tccggaagta tggcaaccct accagccgga gatgtggcct
     3841 caatgatgac cggacctgtg cttgccaagg caaagaccct aacacctgcg gtgcctcctt
     3901 ctccttcggc tgttcctgga gcatgtactt caacggctgc aaatatgctc ggagcaagac
     3961 gccacgaaag ttccgcctca cgggagacaa tccgaaggag gaggaggtgc tccggaatag
     4021 ctttcaggat ctggccactg aagttgctcc cctctacaag cggctcgcac cccaggccta
     4081 tcagaaccag gtgaccaatg aggatgtggc gatcgactgc cgcctggggc tgaaggaagg
     4141 gagacccttc tcaggggtca cagcctgcat ggacttctgt gcccacgccc acaaggacca
     4201 acataacctc tacaatgggt gcactgtggt ctgcaccctg accaaggaag acaatcgctg
     4261 cgtgggccag atccctgagg acgagcaact gcacgtgctg cccctctaca agatggccag
     4321 cacggatgag tttggcagcg aggaaaacca gaacgccaag gtcagtagtg gggccatcca
     4381 ggtgctcaca gcattcccca gagaggtccg gcggctgcct gagcctgcca agtcctgccg
     4441 ccaacggcag ctggaagcca ggaaggcggc ggccgagaag aagaagctgc agaaggagaa
     4501 actgagcacg ccagagaaga tcaagcagga ggccctggag ttggctggag tcaccactga
     4561 cccaggcctg tctctgaagg gtggattgtc ccagcaaagc ctgaagccct ccctcaaggt
     4621 ggagcctcag aaccacttta gctcctttaa gtacagtggc aatgcggtgg tggaaagcta
     4681 ctcggtgctg ggcagctgcc ggccctccga cccctacagc atgagcagtg tgtattccta
     4741 ccattcgcgc tatgcacagc ctggcctggc ctctgtcaac ggcttccact ccaagtacac
     4801 acttccctcc tttggctact atggctttcc atcaagcaac cctgtcttcc cctcccagtt
     4861 cctgggtccc agtgcctggg ggcatggggg cagtggaggc agttttgaga agaagccaga
     4921 cctccatgct ctacacaaca gcctgaaccc agcctacggt ggtgctgagt ttgccgagct
     4981 gccaggtcag gctgttgcca cagacaacca ccaccccatc cctcaccacc agcagcctgc
     5041 ttacccaggc cccaaggaat atctgctacc caaggtcccc cagctccacc cagcatccag
     5101 ggacccctct ccctttgctc agagttccag ttgctacaac agatccatca agcaagagcc
     5161 aatagaccct ctgacccagg ctgagtccat tcccagagac tctgctaaga tgagtagaac
     5221 acccttgccg gaagcatctc agaatggggg acccagtcat ctgtggggac agtactcagg
     5281 aggcccaagc atgtccccga agaggactaa cagtgtaggt ggcaactggg gcgtgttccc
     5341 tccgggggag agccctacca ttgttcccga caagctcaat tcttttgggg ccagctgtct
     5401 cactccttca cacttcccag aaagccagtg gggactgttc actggtgaag gccagcagtc
     5461 ggccccccat gctggagcac ggcttcgagg caagccatgg agcccctgca agtttgggaa
     5521 cggcacctct gccttgactg gtcccagcct aactgagaag ccatggggga tgggaaccgg
     5581 ggatttcaac cccgccctga aaggtggacc tgggttccaa gacaagttgt ggaatcctgt
     5641 gaaggtggag gagggcagga ttcccacacc gggggccaac ccgctagaca aagcctggca
     5701 agcctttggc atgcccttga gctccaacga gaagctattt ggggccctga agtcagagga
     5761 gaaactgtgg gatcccttca gcctggagga ggggacagct gaggagcccc ccagcaaggg
     5821 ggtggtgaag gaagagaaga gtggacccac agtggaagag gaggaggagg aactgtggtc
     5881 ggacagtgaa cacaacttcc tggatgagaa cataggcggg gtggccgtgg cccccgccca
     5941 ttgctccatc ctcatcgagt gtgcccggcg agagctgcat gccaccactc cactcaaaaa
     6001 acccaaccgc tgccacccca cccgcatctc gctggtcttc taccaacaca agaacctcaa
     6061 ccagcccaac cacgggctgg cgctctggga ggccaagatg aagcagctgg cggaacgggc
     6121 gcggcagcgg caagaggagg ccgcacgcct gggcctgggc cagcaggagg ccaagctcta
     6181 cgggaagaag cgaaaatggg ggggtgctat ggtggctgag ccccagcaca aagaaaagaa
     6241 gggggctatc cctacccggc aggcgctggc catgcccaca gactccgcgg tcaccgtgtc
     6301 ctcttacgcc tacacaaagg tcactggccc ctacagccgc tggatctagg tgccaggagc
     6361 cagcgaaggg cgaattctgc agatatccat cacactggcg gccgctcgac tatgggcacc
     6421 aaagaacctg taaacgttat ctttttaaat tgaatgtgca caaataaaag tttggaaaag
     6481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     6541 aaaaaaaaaa aaaaaaaaaa aaatacgcat catacgctgt ggcgtacctc gagtctagag
     6601 ggcccttcga acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc
     6661 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6721 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6781 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6841 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6901 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6961 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7021 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7081 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7141 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7201 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7261 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7321 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7381 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7441 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7561 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7621 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7681 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7741 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     7801 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7861 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7921 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7981 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     8041 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     8101 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     8161 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     8221 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     8281 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     8341 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     8401 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     8461 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     8521 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     8581 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga
     8641 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     8701 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     8761 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8821 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8881 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8941 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9001 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9061 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11101 acgtc
//
