LOCUS       sequence-244174-       10460 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of EGFR E746_S752delinsV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E746_S752delinsV
FEATURES             Location/Qualifiers
     source          1..10460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5420..5444)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5452..5470
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5495..5514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5496..5514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5520..5540)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5594..5635
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5656..6223
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5673..6225
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5817..5834)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6044..6063
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6237..6836
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6237..6256)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6733..6753
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6850..7438
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6903..6923)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7321..7332)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7513..7746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7865..7883)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7951..8055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8056..8124,8125..8916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9087..9675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9576..9595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9829..9846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10086..10107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10122..10127,10128..10145,10146..10152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10160..10176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10165..10187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10184..10200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10184..10200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10250..10269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc
     3481 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc
     3541 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc
     3601 cagaggatgt tcaataactg tgaggtggtc cttgggaacg ccggccatgt gtgccacctg
     3661 tgccatccaa actgcaccta cggatgcact gggccaggtc ttgaaggctg tccaacgaat
     3721 gggcctaaga tcccgtccat cgccactggg atggtggggg ccctcctctt gctgctggtg
     3781 gtggccctgg ggatcggcct cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg
     3841 cggaggctgc tgcaggagag ggagcttgtg gagcctctta cacccagtgg agaagctccc
     3901 aaccaagctc tcttgaggat cttgaaggaa actgaattca aaaagatcaa agtgctgggc
     3961 tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga gaaagttaaa
     4021 attcccgtcg ctatcaaggt tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg
     4081 atggccagcg tggacaaccc ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc
     4141 gtgcagctca tcacgcagct catgcccttc ggctgcctcc tggactatgt ccgggaacac
     4201 aaagacaata ttggctccca gtacctgctc aactggtgtg tgcagatcgc aaagggcatg
     4261 aactacttgg aggaccgtcg cttggtgcac cgcgacctgg cagccaggaa cgtactggtg
     4321 aaaacaccgc agcatgtcaa gatcacagat tttgggctgg ccaaactgct gggtgcggaa
     4381 gagaaagaat accatgcaga aggaggcaaa gtgcctatca agtggatggc attggaatca
     4441 attttacaca gaatctatac ccaccagagt gatgtctgga gctacggggt gaccgtttgg
     4501 gagttgatga cctttggatc caagccatat gacggaatcc ctgccagcga gatctcctcc
     4561 atcctggaga aaggagaacg cctccctcag ccacccatat gtaccatcga tgtctacatg
     4621 atcatggtca agtgctggat gatagacgca gatagtcgcc caaagttccg tgagttgatc
     4681 atcgaattct ccaaaatggc ccgagacccc cagcgctacc ttgtcattca gggggatgaa
     4741 agaatgcatt tgccaagtcc tacagactcc aacttctacc gtgccctgat ggatgaagaa
     4801 gacatggacg acgtggtgga tgccgacgag tacctcatcc cacagcaggg cttcttcagc
     4861 agcccctcca cgtcacggac tcccctcctg agctctctga gtgcaaccag caacaattcc
     4921 accgtggctt gcattgatag aaatgggctg caaagctgtc ccatcaagga agacagcttc
     4981 ttgcagcgat acagctcaga ccccacaggc gccttgactg aggacagcat agacgacacc
     5041 ttcctcccag tgcctgaata cataaaccag tccgttccca aaaggcccgc tggctctgtg
     5101 cagaatcctg tctatcacaa tcagcctctg aaccccgcgc ccagcagaga cccacactac
     5161 caggaccccc acagcactgc agtgggcaac cccgagtatc tcaacactgt ccagcccacc
     5221 tgtgtcaaca gcacattcga cagccctgcc cactgggccc agaaaggcag ccaccaaatt
     5281 agcctggaca accctgacta ccagcaggac ttctttccca aggaagccaa gccaaatggc
     5341 atctttaagg gctccacagc tgaaaatgca gaatacctaa gggtcgcgcc acaaagcagt
     5401 gaatttattg gagcatagaa cccagctttc ttgtacaaag tggtgatatc caattaaccc
     5461 tcactaaagg gtaagagtat tgaaattagt aagaccctat agtgagtcgt attaccaccc
     5521 aacttttcta tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg
     5581 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     5641 cggttagtaa tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata
     5701 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5761 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5821 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5881 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5941 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6001 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6061 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6121 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6181 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatga
     6241 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6301 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6361 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6421 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6481 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6541 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6601 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6661 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6721 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6781 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaatcg
     6841 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6901 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6961 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7021 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7081 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7141 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7201 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7261 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7321 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7381 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7441 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7501 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7561 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7621 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7681 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7741 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7801 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     7861 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7921 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7981 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8041 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8101 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8161 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8221 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8281 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     8341 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8401 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8461 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8521 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8581 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8641 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8701 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8761 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8821 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8881 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8941 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9001 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9061 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9121 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9181 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9241 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9301 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9361 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9421 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9481 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9541 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9601 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9661 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9721 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9781 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9841 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9901 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9961 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10021 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10081 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10141 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10201 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10261 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    10321 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    10381 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10441 tgtactacaa acttagtagt
//
