LOCUS       sequence-244261-        4274 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Full MCS, lacks araC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Santos-Moreno J, Schaerli Y
  TITLE     A Framework for the Modular and Combinatorial Assembly of Synthetic 
            Gene Circuits.
  JOURNAL   ACS Synth Biol. 2019 Jul 19;8(7):1691-1697. doi: 
            10.1021/acssynbio.9b00174. Epub 2019 Jun 24.
  PUBMED    31185158
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00174"; journalName: "ACS Synth Biol"; date: 
            "2019-07-19- 19"; volume: "8"; issue: "7"; pages: "1691-1697"
COMMENT     Sequence Label: pC-0_v6
FEATURES             Location/Qualifiers
     source          1..4274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1045..1063)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1582..2532)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2580..2802
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3294..3388)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3414..4205,4206..4274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4037..4056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gaattcatag gatagattct ggaaacttta ccgtccgagc tcaggcttac
      181 cttactcgag caataaacag ttgatagggc ttctccgtta ccatggttca gccaaaaaac
      241 ttaagaccgc cggtcttgtc cactaccttg cagtaatgcg gtggacagga tcggcggttt
      301 tcttttctct tctcaattct tctgacctgt aacgaataat agatagtaaa gtagtctccg
      361 attgagtttt ctctgccgag tcccacccag ttctgtgatt tcagtaagtt ggtaattgat
      421 acactgttgc gagaactgct gcctggtagt agataggttg ttattgagta agaaggtaaa
      481 gtgaacgaaa tccctgaaac tgagactgta gaaaataagc ttgtccagac tattggatcc
      541 aagagatttc tacacgattg agcactgtct cctgcaggct cggtaccaaa ttccagaaaa
      601 gaggcctccc gaaagggggg ccttttttcg ttttggtcct aatagataaa ggataggtct
      661 ggtagtgttg ttcgttctcg caggtaaatc aataatactc agcagttccg tagacttttc
      721 agtgggacag ggtagcgata acagatagat tgtaataaga cacagtaggt gctcgtagtt
      781 gcgtgaagag aaccgctcag gaaatccagt cagaagtatt ggtaatcgtt gaaaactcag
      841 tcgacgcact tactgaagac gtcctattac actcgtcgtt ggaaactgaa gatgcggccg
      901 cggaaacaca gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg accaaaggta
      961 gcgaacgacg agtcactgtt gaggataaat actttctcta ctaggcgcct gttacacagg
     1021 tcctcagcgg cgcgcctgct gccaccgctg agcaataact agcgtacggg ttttgctgcc
     1081 cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc cggcttcagc
     1141 cggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga
     1201 ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc
     1261 aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt
     1321 tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct
     1381 gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc
     1441 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
     1501 gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
     1561 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
     1621 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
     1681 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
     1741 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt
     1801 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
     1861 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
     1921 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
     1981 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
     2041 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
     2101 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
     2161 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
     2221 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
     2281 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
     2341 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     2401 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     2461 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     2521 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     2581 ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg
     2641 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     2701 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     2761 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     2821 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     2881 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     2941 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     3001 cggctctggc agtgaatggg ggtaaatggc actacaggct ccttttatgg attcatgcaa
     3061 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     3121 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     3181 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     3241 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca
     3301 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     3361 gtcattactg gatctatcaa caggagtcca agcgcgtaaa cttggtctga cagttaccaa
     3421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4261 tgttgaatac tcat
//
