LOCUS       sequence-244270-        9575 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Positive RNAi control for pGAPi-based RNAi silencing in tandem with 
            APT transcript segment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Orr RG, Foley SJ, Sherman CA, Abreu I, Galotto G, Liu B, 
            Gonzalez-Guerrero M, Vidali L
  TITLE     Robust survival-based RNAi of gene families using in tandem 
            silencing of adenine phosphoribosyltransferase.
  JOURNAL   Plant Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 
            10.1104/pp.20.00865.
  PUBMED    32764132
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2020 Aug 6. pii: pp.20.00865. doi: 10.1104/pp.20.00865."
COMMENT     Sequence Label: MyoUTipGAPi
FEATURES             Location/Qualifiers
     source          1..9575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2389..2413
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3237..3261)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3652..3676
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4500..4524)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4943..5195
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5228..5572
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5515..5535
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5602..6627
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5602..6627
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6696..6872
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7429..7445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7429..7445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7442..7464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7453..7469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7477..7483,7484..7501,7502..7507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7522..7543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7660..7677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7831..8419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7911..7930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8590..9381,9382..9450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9213..9232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9451..9555)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc
       61 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga
      121 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca
      181 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct
      241 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca
      301 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt
      361 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt
      421 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta
      481 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga
      541 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca
      601 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
      661 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
      721 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcacgg
      781 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat
      841 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac
      901 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc
      961 gtcctccccc cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg
     1021 cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc
     1081 tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc
     1141 cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt
     1201 tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata
     1261 tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat
     1321 gatgtggtct ggttgggcgg tcgttctaga tcggagtaga attaattctg tttcaaacta
     1381 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     1441 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     1501 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     1561 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     1621 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     1681 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     1741 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     1801 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     1861 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     1921 cttttgtcga tgctcaccct gttgtttggt gttacttctg cgccagtgcc aagttaattc
     1981 gagctcaagc tttaccgtgc gccacttgac acagactgga aatattctcg ttcacacagt
     2041 gctagggctt cagggtgtga gccgtggcgc atcttgtgga cttgccgtgg cgagctacct
     2101 ccacgctccc gcttccgcaa ttttgttgca gtgctttgga ttctcgtggt tgctcgtctc
     2161 aggaagtgaa gatgtcggcc tccaaggatg gcgaccctcg gatccagtat atctcggaca
     2221 gcatccgtac cattcctgat tttcctcaca aaggcattat gttccgagat gtgacgacgt
     2281 tgctgttgga tcataaggct ttcaaagaca cgatcgacat ctttgttgag cgttaccggg
     2341 accagaaggt ggacgtcatt gtgggaattg aagctcgagg tatttaaatc cactttgtac
     2401 aagaaagctg ggtcggcgcg cccacccttg cgccacgcga aactcctgat tgcgatcacc
     2461 tcatctacac tggtgtggcc tgcacaagat ttaaaacacg acaactcgca cgctccacag
     2521 acggagcccc cgctccacag cactgatcca caaactccgc gctcactccc ccgctaatca
     2581 cctacgagcc acccctcgcc cgcctcctcc ctcctgatcc agcaaatatt gcaacgcacg
     2641 gtctatctga ccgcttccac ggcgagaaaa ataaaaataa aagtaaaaaa aaaatgcccg
     2701 ggcttgatga tgttccttga gaaacaatct gcgcgcctct ccgcggctcc gagagggacc
     2761 gaggcgaagt attgattcga gtcagtgtgg agagcagatg cggaggggag ggaggggaag
     2821 ggggacagca ctaacattac gggtgaaggg ggcggccgcg ggaattcgat tctttttcaa
     2881 ttgtgatttc gcgttggttg tgttggtgct gtctcctcct cctcctcgtc atctcctctt
     2941 cagacgcaca gtcgaagcta cacccccccc cccaattact gtggttggct ggcacctagg
     3001 atcacgcacg cacgctctct accgggtctt catcgacact ttcatttctc ccaggctagg
     3061 agaggttttg ttctttgctg tggaagtgtg ctgctgccgc tgctcagggg aggggctgct
     3121 gctgtgagta gcaagattcg ttagattacg aacgaggacg acgacgagga acacgagggg
     3181 acgctttctt ggtgattggg acgggggttc aatcactagt gaattcgcgg ccgcggagcc
     3241 tgcttttttg tacaaacttg tgattacgta cagatgaaca tggcatcgtg gtgattgatg
     3301 aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc
     3361 cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg
     3421 cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg
     3481 ccaacgaacc ctcgagggat acccgtccgc aagtgcacgg gaatatttcg ccactggcgg
     3541 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
     3601 acgctcacac cgataccatc agcgatctct ttgatgccta ggtacgtaat cacaagtttg
     3661 tacaaaaaag caggctccgc ggccgcgaat tcactagtga ttgaaccccc gtcccaatca
     3721 ccaagaaagc gtcccctcgt gttcctcgtc gtcgtcctcg ttcgtaatct aacgaatctt
     3781 gctactcaca gcagcagccc ctcccctgag cagcggcagc agcacacttc cacagcaaag
     3841 aacaaaacct ctcctagcct gggagaaatg aaagtgtcga tgaagacccg gtagagagcg
     3901 tgcgtgcgtg atcctaggtg ccagccaacc acagtaattg gggggggggg tgtagcttcg
     3961 actgtgcgtc tgaagaggag atgacgagga ggaggaggag acagcaccaa cacaaccaac
     4021 gcgaaatcac aattgaaaaa gaatcgaatt cccgcggccg cccccttcac ccgtaatgtt
     4081 agtgctgtcc cccttcccct ccctcccctc cgcatctgct ctccacactg actcgaatca
     4141 atacttcgcc tcggtccctc tcggagccgc ggagaggcgc gcagattgtt tctcaaggaa
     4201 catcatcaag cccgggcatt ttttttttac ttttattttt atttttctcg ccgtggaagc
     4261 ggtcagatag accgtgcgtt gcaatatttg ctggatcagg agggaggagg cgggcgaggg
     4321 gtggctcgta ggtgattagc gggggagtga gcgcggagtt tgtggatcag tgctgtggag
     4381 cgggggctcc gtctgtggag cgtgcgagtt gtcgtgtttt aaatcttgtg caggccacac
     4441 cagtgtagat gaggtgatcg caatcaggag tttcgcgtgg cgcaagggtg ggcgcgccga
     4501 cccagctttc ttgtacaaag tgggtttaaa tacctcgagc ttcaattccc acaatgacgt
     4561 ccaccttctg gtcccggtaa cgctcaacaa agatgtcgat cgtgtctttg aaagccttat
     4621 gatccaacag caacgtcgtc acatctcgga acataatgcc tttgtgagga aaatcaggaa
     4681 tggtacggat gctgtccgag atatactgga tccgagggtc gccatccttg gaggccgaca
     4741 tcttcacttc ctgagacgag caaccacgag aatccaaagc actgcaacaa aattgcggaa
     4801 gcgggagcgt ggaggtagct cgccacggca agtccacaag atgcgccacg gctcacaccc
     4861 tgaagcccta gcactgtgtg aacgagaata tttccagtct gtgtcaagtg gcgcacggta
     4921 aagcttggta cccctactcc aagatcgttc aaacatttgg caataaagtt tcttaagatt
     4981 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     5041 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     5101 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     5161 attatcgcgc gcggtgtcat ctatgttact agatcgggga tacagtctca gaagaccaaa
     5221 gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga ttccattgcc
     5281 cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc
     5341 atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt ggtcccaaag
     5401 atggaccccc accccgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     5461 gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat cccactatcc
     5521 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cagcccaagc
     5581 atggagaacc gacctgcagg catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     5641 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     5701 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     5761 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     5821 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     5881 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     5941 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6001 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6061 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6121 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6181 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6241 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6301 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6361 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6421 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6481 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     6541 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     6601 agcactcgtc cgagggcaaa ggaatgagcg ggactctggg gttcggacgg tacgctgaaa
     6661 tcaccagtct cttctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta
     6721 gtttcccgat aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     6781 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     6841 taaaaccaaa atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt
     6901 gaatataaac cagacacgag acgactaaac ctggagccca gacgccgttc gaagctagaa
     6961 gtaccgctta ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga
     7021 ctcccccgta ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg
     7081 aaggataagg ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct
     7141 actatactta tactatacgc taagggaatg cttgtattta taccctatac cccctaataa
     7201 ccccttatca atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc
     7261 catgaatagg tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt
     7321 tcttaactct aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac
     7381 ggggatcgca tgcgatatct gcgagagatc tagactggcg taatcatggt catagctgtt
     7441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7561 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7621 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     8041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8881 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9181 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9541 aataggggtt ccgcgcacat ttccccgaaa agtgc
//
