LOCUS       sequence-244686-        8596 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For stable expression sgRNA resistant Flag tagging mLST8_.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
  TITLE     Disruption of the Scaffolding Function of mLST8 Selectively Inhibits
            mTORC2 Assembly and Function and Suppresses mTORC2-Dependent Tumor 
            Growth In Vivo.
  JOURNAL   Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 
            10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13.
  PUBMED    31085701
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-18-3658"; journalName: "Cancer Res"; date: 
            "2019-07-1- 1"; volume: "79"; issue: "13"; pages: "3178-3184"
COMMENT     Sequence Label: pLentiCMV-Blast mLST8-Flag
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    227..343
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        366..669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        670..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     824..844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     870..894
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1934..1957
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1968..2556
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2021..2041)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2439..2450)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2607..2627)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2612..2941
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2792..2927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2989..3036
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3055..3453
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3540..3773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3845..3966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3882..3901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3936..3955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4006..4141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4161..4180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4162..4180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4190..4207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4190..4206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4199..4221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4348..4803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4435..4454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4645..4666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4829..4933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4934..5002,5003..5794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5152..5171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5965..6553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6454..6473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6707..6724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6841..6862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6877..6882,6883..6900,6901..6907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6915..6931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6920..6942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6939..6955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6939..6955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6974..6994
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6976..6994
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7022..7248
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7249..7429
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7476..7601
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8094..8327
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8512..8556
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
       61 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
      121 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
      181 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
      241 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      301 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata agcttgggag
      361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      841 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      901 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag ccaccatgaa
      961 cacctcccca ggcacggtgg gcagtgaccc ggtcatcctg gcaaccgccg gctacgacca
     1021 caccgtgcgc ttctggcagg cccacagcgg catctgcacc cggacggtgc agcaccagga
     1081 ctcccaggtg aatgccttgg aggtcacacc ggaccgcagc atgattgctg ctgcaggtta
     1141 ccagcacatc cgcatgtatg atctcaactc caataaccct aaccccatca tcagctacga
     1201 cggcgtcaac aagaacatcg cgtctgtggg cttccacgaa gacggccgct ggatgtacac
     1261 gggcggcgag gactgcacag ccaggatctg ggacctcagg tcccggaacc tgcagtgcca
     1321 gcggatcttc caggtgaacg cacccattaa ctgcgtgtgc ctgcacccca accaggcaga
     1381 gctcatcgtg ggtgaccaga gcggggctat ccacatctgg gacttgaaaa cagaccacaa
     1441 cgagcagctg atccctgagc ccgaggtctc catcacgtcc gcccacatcg atcccgacgc
     1501 cagctacatg gcagctgtca atagcaccgg aaactgctat gtctggaatc tgaccggtgg
     1561 cattggtgac gaggtgaccc agctcatccc caagactaag atccctgccc acacgcgcta
     1621 cgccctgcag tgtcgcttca gccccgactc cacgctcctc gccacctgct cggctgatca
     1681 gacgtgcaag atctggagga cgtccaactt ctccctgatg acggagctga gcatcaagag
     1741 cggcaacccc ggggagtcct cccgcggctg gatgtggggc tgcgccttct cgggggactc
     1801 ccagtacatc gtcactgctt cctcggacaa cctggcccgg ctctggtgtg tggagactgg
     1861 agagatcaag agagagtatg gcggccacca gaaggctgtt gtctgcctgg ccttcaatga
     1921 cagtgtgctg ggcgactaca aagacgatga cgacaagtaa tgtcgacaat caacctctgg
     1981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2041 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2101 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2161 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2221 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2281 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2341 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     2401 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2461 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     2521 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctgcag atatccggtt
     2581 agtaatgagt ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     2641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     2761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2881 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2941 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     3001 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     3061 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     3121 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     3181 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     3241 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     3301 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     3361 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     3421 ttgctgccct ctggttatgt gtgggagggc taagcacaat tcgagctcgg tacctttaag
     3481 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3541 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     3601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     3781 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     3841 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3901 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3961 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     4021 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4081 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4141 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     4201 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4261 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4321 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     4801 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4861 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4921 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4981 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5041 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5101 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5161 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5221 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5281 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5341 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5401 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5461 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5521 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5581 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5641 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5701 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5761 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5821 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5881 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5941 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6001 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6061 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6121 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6181 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6241 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6301 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6361 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6421 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6481 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6541 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6601 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6661 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6721 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6781 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6841 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6901 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6961 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7021 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     7081 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     7141 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     7201 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     7261 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7321 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7381 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7441 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     7501 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     7561 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     7621 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     7681 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     7741 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     7801 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     7861 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     7921 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     7981 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8041 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8101 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8161 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8221 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8281 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8341 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     8401 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     8461 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     8521 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     8581 tggctgtggt atataa
//
