LOCUS       sequence-244732-        9206 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Reporter plasmid with constitutively expressed mCherry and YFP 
            (eCitrine).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Monteiro F, Hubmann G, Takhaveev V, Vedelaar SR, Norder J, Hekelaar 
            J, Saldida J, Litsios A, Wijma HJ, Schmidt A, Heinemann M
  TITLE     Measuring glycolytic flux in single yeast cells with an orthogonal 
            synthetic biosensor.
  JOURNAL   Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071.
  PUBMED    31885198
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2019-12"; volume: "15(12):e9071. doi"; pages: " 
            10.15252/msb.20199071"
COMMENT     Sequence Label: pTEF6-7
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2621..2642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2754..2774
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2827..2860
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            2915..4271
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2915..3258
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3259..4068
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFPPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3326..3345)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      4074..4271
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    4334..4367
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4399..4796
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4809..5525
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5528..5544)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5529..5545)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5547..5794
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5563..5581)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(5812..5977)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5898..5922
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(6000..6710)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6278..6297)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6547..6565
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(6723..7121)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6813..6824
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7153..7186)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7222..7241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7223..7241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7251..7268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7251..7267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7260..7282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7408..7863
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7495..7514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7705..7726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7994..8797)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8798..9013)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9037..9056)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9156..9178
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     2761 accctcacta aagggaacaa aagctgcagc tgaagcttcg tacgctgcag gtcgacaacc
     2821 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga gatctgttta
     2881 gctagcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
     2941 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     3001 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     3061 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     3121 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     3181 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     3241 atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat
     3301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     3361 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     3421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     3481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     3541 ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca
     3601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     3661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3781 caagtctgga aagaaatgca taagcttttg ccattcccac cggattcagt cgtcactcat
     3841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     4021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agtactgaca
     4081 ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg
     4141 ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc
     4201 cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg
     4261 tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctctc
     4321 gagaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtgatatcag
     4381 atccactagt ggcctatgca tagcttcaga atgtttctac tcctttttta ctcttccaga
     4441 ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa
     4501 tttcccctct ttcttcctct agggtgtcgt taactacccg tactaaaggt ttggaaaaaa
     4561 aaaagagacc gcctcgtttc tttttcttcg ccgaaaaagg caataaaaat ttttatcacg
     4621 tttctttttc ttgaaaattt tttttttgat ttttttctct ttcgatgacc tcccattgat
     4681 atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt cttgctctat
     4741 tacaactttt tttacttctt gctcattaga aagaaagcat agcaatctaa tctaagtttt
     4801 ctagaaaaat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat
     4861 tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta
     4921 cttacggtaa attgacctta aaatttattt gtactactgg taaattgcca gttccatggc
     4981 caaccttagt cactacttta ggttatggtt tgatgtgttt tgctagatac ccagatcata
     5041 tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta
     5101 tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata
     5161 ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag
     5221 gtcacaaatt ggaatacaac tataactctc acaatgttta catcatggct gacaaacaaa
     5281 agaatggtat caaagttaac ttcaaaatta gacacaacat tgaagatggt tctgttcaat
     5341 tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca
     5401 accattactt atcctatcaa tctgccttat ccaaagatcc aaacgaaaag agagaccaca
     5461 tggtcttgtt agaatttgtt actgctgctg gtattaccca tggtatggat gaattgtaca
     5521 aataaatcga taccgtcgac ctcgagtcat gtaattagtt atgtcacgct tacattcacg
     5581 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     5641 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     5701 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     5761 gaaggttttg ggacgctcga aggctttaat ttgcggccgg taccagcgta cgagcgacct
     5821 catgctatac ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt
     5881 ttcgttttaa aacctaagag tcactttaaa atttgtatac acttattttt tttataactt
     5941 atttaataat aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgc
     6001 tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt
     6061 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag
     6121 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag
     6181 tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg
     6241 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
     6301 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg
     6361 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
     6421 ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg
     6481 taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg
     6541 ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
     6601 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
     6661 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat ttttctagaa
     6721 aacttagatt agattgctat gctttctttc taatgagcaa gaggtaaaaa aagttgtagt
     6781 agagcaagaa aagtgggact gaaacttggg aaattgaagg ccgtttatta acttaaatat
     6841 cgatgggagg tcatcgaaag agaaaaaaat caaaaaaaga attttcaaga aaaagaaacg
     6901 tgataaaaat ttttattgcc tttttcgacg aagaaaaaga aacgaggcgg tctctttttt
     6961 cttttccaaa cctttagtac gggtaattaa cgacacccta gaggaagaaa gaggggaaat
     7021 ttagtatgct gtgcttgggt gttttgaagt ggtacggcga tgcgcggagt ccgagaagat
     7081 ctggaagagt aaaagaggag tagaaacgtt tcgaagctat gcataggcca ctagtggatc
     7141 tgatatcacc taataacttc gtatagcata cattatacga agttatatta agggttctcg
     7201 agagctcggg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7261 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7321 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7381 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     7441 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7501 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7561 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7621 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7681 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7741 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7801 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7861 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta
     7921 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
     7981 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
     8041 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
     8101 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
     8161 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
     8221 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct
     8281 tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca
     8341 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca
     8401 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg
     8461 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt
     8521 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg
     8581 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg
     8641 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac
     8701 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat
     8761 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg
     8821 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac
     8881 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg
     8941 agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa
     9001 atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca atctgctctg atgccgcata
     9061 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     9121 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     9181 ttcaccgtca tcaccgaaac gcgcga
//
