LOCUS       sequence-24564-e         827 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Harvey CD, Yasuda R, Zhong H, Svoboda K
  TITLE     The spread of Ras activity triggered by activation of a single 
            dendritic spine.
  JOURNAL   Science. 2008 Jul 4. 321(5885):136-40.
  PUBMED    18556515
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jul 4. 321(5885):136-40."
COMMENT     Sequence Label: paGFP-HRas
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(302..553)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(344..365)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     694..713
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cnnnntggtg gagtcctcaa aagacttggt gttgttgatg gcaaacacac acaggaagcc
       61 ctccccggtg cgcatgntac tggtcccgca tggcgctgta ctcctcctgg ccggcggtat
      121 ccaggatgtc caacaggcac gtctccccat caatgaccac ctgcttccgg taggaatcct
      181 ctatagtggg gtcgtattcg tccacaaaat ggttctggat cagctggatg gtcagcgcac
      241 tcttgcccac accgccggcg cccaccacca ccagcttata ttccgtcatc tcgagtccgg
      301 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      361 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactggtgg ctcaggtagt
      421 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      481 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
      541 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      601 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      661 ngggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      721 aatatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaaaan tcgtgctgct
      781 natgtggtcg gggtagcggc tgaacactgc acgccgtaac tgaagtg
//
