LOCUS       sequence-245760-        8469 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Recombinational donor/master vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC,
            Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, 
            Durocher D
  TITLE     53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin 
            mark.
  JOURNAL   Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 
            2013 Jun 12.
  PUBMED    23760478
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12318"; journalName: "Nature"; date: "2013-07-4- 4"; 
            volume: "499"; issue: "7456"; pages: "50-4"
COMMENT     Sequence Label: pDONR-53BP1 1-1972 WT
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1220..1319
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(7245..7344)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7361..7380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7362..7380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7385..7401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7385..7401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7503..7522)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7514..8323
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7581..7600)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1261 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc
     1321 tatggaccct actggaagtc agttggattc agatttctct cagcaagaca ctccttgcct
     1381 gataattgaa gattctcagc ctgaaagcca ggttctagag gatgattctg gttctcactt
     1441 cagtatgcta tctcgacacc ttcctaatct ccagacgcac aaagaaaatc ctgtgttgga
     1501 tgttgtgtcc aatcctgaac aaacagctgg agaagaacga ggagacggta atagtgggtt
     1561 caatgaacat ttgaaagaaa acaaggttgc agaccctgtg gattcttcta acttggacac
     1621 atgtggttcc atcagtcagg tcattgagca gttacctcag ccaaacagga caagcagtgt
     1681 tctgggaatg tcagtggaat ctgttcctgc tgtggaggaa gagaagggag aagagttgga
     1741 acagaaggag aaagagaagg aagaagatac ttcaggcaat actacacatt cccttggtgc
     1801 tgaagatact gcctcatcac agttgggttt tggggttctg gaactctccc agagccagga
     1861 tgttgaggaa aatactgtgc catatgaagt ggacaaagag cagctacaat cagtaaccac
     1921 caactctggt tataccaggc tgtctgatgt ggatgctaat actgcaatta agcatgaaga
     1981 acagtccaac gaagatatcc ccatagcaga acagtccagc aaggacatcc ctgtgacagc
     2041 acagcccagt aaggatgtac atgttgtaaa agagcaaaat ccaccacctg caaggtcaga
     2101 ggacatgcct tttagcccca aagcatctgt tgctgctatg gaagcaaaag aacagttgtc
     2161 tgcacaagaa cttatggaaa gtggactgca gattcagaag tcaccagagc ctgaggtttt
     2221 gtcaactcag gaagacttgt ttgaccagag caataaaaca gtatcttctg atggttgctc
     2281 tactccttca agggaggaag gtgggtgttc tttggcttcc actcctgcca ccactctgca
     2341 tctcctgcag ctctctggtc agaggtccct tgttcaggac agtctttcca cgaattcttc
     2401 agatcttgtt gctccttctc ctgatgcttt ccgatctact ccttttatcg tccctagcag
     2461 tcccacagag caagaaggga gacaagataa gccaatggac acgtcagtgt tatctgaaga
     2521 aggaggagag ccttttcaga agaaacttca aagtggtgaa ccagtggagt tagaaaaccc
     2581 ccctctcctg cctgagtcca ctgtatcacc acaagcctca acaccaatat ctcagagcac
     2641 accagtcttc cctcctgggt cacttcctat cccatcccag cctcagtttt ctcatgacat
     2701 ttttattcct tccccaagtc tggaagaaca atcaaatgat gggaagaaag atggagatat
     2761 gcatagttca tctttgacag ttgagtgttc taaaacttca gagattgaac caaagaattc
     2821 ccctgaggat cttgggctat ctttgacagg ggattcttgc aagttgatgc tttctacaag
     2881 tgaatatagt cagtccccaa agatggagag cttgagttct cacagaattg atgaagatgg
     2941 agaaaacaca cagattgagg atacggaacc catgtctcca gttctcaatt ctaaatttgt
     3001 tcctgctgaa aatgatagta tcctgatgaa tccagcacag gatggtgaag tacaactgag
     3061 tcagaatgat gacaaaacaa agggagatga tacagacacc agggatgaca ttagtatttt
     3121 agccactggt tgcaagggca gagaagaaac ggtagcagaa gatgtttgta ttgatctcac
     3181 ttgtgattcg gggagtcagg cagttccgtc accagctact cgatctgagg cactttctag
     3241 tgtgttagat caggaggaag ctatggaaat taaagaacac catccagagg aggggtcttc
     3301 agggtctgag gtggaagaaa tccctgagac accttgtgaa agtcaaggag aggaactcaa
     3361 agaagaaaat atggagagtg ttccgttgca cctttctctg actgaaactc agtcccaagg
     3421 gttgtgtctt caaaaggaaa tgccaaaaaa agaatgctca gaagctatgg aagttgaagc
     3481 cagtgtgatt agtattgatt cccctcaaaa gttggcaata cttgaccaag aattggaaca
     3541 taaggaacag gaagcttggg aagaagctac ttcagaggac tccagtgttg tcattgtaga
     3601 tgtgaaagag ccatctccca gagttgatgt ttcttgtgaa cctttggagg gagtggagaa
     3661 gtgctcagat tcccagtcat gggaggatat tgctccagaa atagaaccat gtgctgagaa
     3721 tagattagac accaaggaag aaaagagtgt agaatatgaa ggagatctga aatcagggac
     3781 tgcagaaaca gaacctgtag agcaagattc ttcacagcct tccttacctt tagtacgcgc
     3841 tgacgacccg cttagacttg accaggagtt gcagcagccc caaactcagg agaaaacaag
     3901 taattcatta acagaagact caaaaatggc taatgcaaag cagctaagct cagatgcaga
     3961 ggcccagaag ctggggaagc cctctgccca tgcctcacaa agcttctgtg aaagttctag
     4021 tgaaacccca tttcatttca ctttgcctaa agaaggtgat atcatcccac cattgactgg
     4081 tgcaacccca cctcttattg ggcacctaaa attggagccc aagagacaca gtactcctat
     4141 tggtattagc aactatccag aaagcaccat agcaaccagt gatgtcatgt ctgaaagcat
     4201 ggtggagacc catgatccca tacttgggag tggaaaaggg gattctgggg ctgccccaga
     4261 cgtggatgat aaattatgtc taagaatgaa actggttagt cctgagactg aggcgagtga
     4321 agagtctttg cagttcaacc tggaaaagcc tgcaactggt gaaagaaaaa atggatctac
     4381 tgctgttgct gagtctgttg ccagtcccca gaagaccatg tctgtgttga gctgtatctg
     4441 tgaagccagg caagagaatg aggctcgaag tgaggatccc cccaccacac ccatcagggg
     4501 gaacttgctc cactttccaa gttctcaagg agaagaggag aaagaaaaat tggagggtga
     4561 ccatacaatc aggcagagtc aacagcctat gaagcccatt agtcctgtca aggaccctgt
     4621 ttctcctgct tcccagaaga tggtcataca agggccatcc agtcctcaag gagaggcaat
     4681 ggtgacagat gtgctagaag accagaaaga aggacggagt actaataagg aaaatcctag
     4741 taaggccttg attgaaaggc ccagccaaaa taacatagga atccaaacca tggagtgttc
     4801 cttgagggtc ccagaaactg tttcagcagc aacccagact ataaagaatg tgtgtgagca
     4861 ggggaccagt acagtggacc agaactttgg aaagcaagat gccacagttc agactgagag
     4921 ggggagtggt gagaaaccag tcagtgctcc tggggatgat acagagtcgc tccatagcca
     4981 gggagaagaa gagtttgata tgcctcagcc tccacatggc catgtcttac atcgtcacat
     5041 gagaacaatc cgggaagtac gcacacttgt cactcgtgtc attacagatg tgtattatgt
     5101 ggatggaaca gaagtagaaa gaaaagtaac tgaggagact gaagagccaa ttgtagagtg
     5161 tcaggagtgt gaaactgaag tttccccttc acagactggg ggctcctcag gtgacctggg
     5221 ggatatcagc tccttctcct ccaaggcatc cagcttacac cgcacatcaa gtgggacaag
     5281 tctctcagct atgcacagca gtggaagctc agggaaagga gccggaccac tcagagggaa
     5341 aaccagcggg acagaacccg tagattttgc cttacccagc tcccgaggag gcccaggaaa
     5401 actgagtcct agaaaagggg tcagtcagac agggacgcca gtgtgtgagg aggatggtga
     5461 tgcaggcctt ggcatcagac agggagggaa ggctccagtc acgcctcgtg ggcgtgggcg
     5521 aaggggccgc ccaccttctc ggaccactgg aaccagagaa acagctgtgc ctggcccctt
     5581 gggcatagag gacatttcac ctaacttgtc accagatgat aaatccttca gccgtgtcgt
     5641 gccccgagtg ccagactcca ccagacgaac agatgtgggt gctggtgctt tgcgtcgtag
     5701 tgactctcca gaaattcctt tccaggctgc tgctggccct tctgatggct tagatgcctc
     5761 ctctccagga aatagctttg tagggctccg tgttgtagcc aagtggtcat ccaatggcta
     5821 cttttactct gggaaaatca cacgagatgt cggagctggg aagtataaat tgctctttga
     5881 tgatgggtac gaatgtgatg tgttgggcaa agacattctg ttatgtgacc ccatcccgct
     5941 ggacactgaa gtgacggccc tctcggagga tgagtatttc agtgcaggag tggtgaaagg
     6001 acataggaag gagtctgggg aactgtacta cagcattgaa aaagaaggcc aaagaaagtg
     6061 gtataagcga atggctgtca tcctgtcctt ggagcaagga aacagactga gagagcagta
     6121 tgggcttggc ccctatgaag cagtaacacc tcttacaaag gcagcagata tcagcttaga
     6181 caatttggtg gaagggaagc ggaaacggcg cagtagcgtc agctccccag ccacccctac
     6241 tgcctccagt agcagcagca caacccctac ccgaaagatc acagaaagtc ctcgtgcctc
     6301 catgggagtt ctctcaggca aaagaaaact tatcacttct gaagaggaac ggtcccctgc
     6361 caagcgaggt cgcaagtctg ccacagtaaa acctggtgca gtaggggcag gagagtttgt
     6421 gagcccctgt gagagtggag acaacaccgg tgaaccctct gccctggaag agcagagagg
     6481 gcctttgcct ctcaacaaga ccttgtttct gggctacgca tttctcctta ccatggccac
     6541 aaccagtgac aagttggcca gccgctccaa actgccagat ggtcctacag gaagcagtga
     6601 agaagaggag gaatttttgg aaattcctcc tttcaacaag cagtatacag aatcccagct
     6661 tcgagcagga gctggctata tccttgaaga tttcaatgaa gcccagtgta acacagctta
     6721 ccagtgtctt ctaattgcgg atcagcattg tcgaacccgg aagtacttcc tgtgccttgc
     6781 cagtgggatt ccttgtgtgt ctcatgtctg ggtccatgat agttgccatg ccaaccagct
     6841 ccagaactac cgtaattatc tgttgccagc tgggtacagc cttgaggagc aaagaattct
     6901 ggactggcaa ccccgtgaaa atcctttcca gaatctgaag gtactcttgg tatcagacca
     6961 acagcagaac ttcctggagc tctggtctga gatcctcatg actggtggtg cagcctctgt
     7021 gaagcagcac cattcaagtg cccataacaa agatattgct ttaggggtat ttgatgtggt
     7081 ggtgacggac ccctcatgcc cagcctcggt gctgaagtgt gctgaagcat tgcagctgcc
     7141 tgtggtgtca caagagtggg tgatccagtg cctcattgtt ggggagagaa ttggattcaa
     7201 gcagcatcca aaatataaac acgattatgt ttctcactaa agggacccag ctttcttgta
     7261 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     7321 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     7381 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     7441 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     7501 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     7561 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     7621 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     7681 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     7741 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     7801 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     7861 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     7921 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     7981 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     8041 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     8101 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     8161 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     8221 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     8281 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     8341 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     8401 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8461 aggatcttc
//
