LOCUS       sequence-245949-        9454 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of Tools for Targeted Gene Expression in Drosophila.
  JOURNAL   Genetics. 2010 Aug 9. ():.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2010 Aug 9. ():."
COMMENT     Sequence Label: pBPGAL4.2::VP16Uw
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(107..112,113..130,131..137)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             191..850
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(257..276)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1192..1497
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1376..1395
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1538..1662)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2824..2840)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2840)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(3851..4465,4536..4667,4871..5186,5248..5902,
                     5977..6250,6652..6723))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7175..7193
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7418..7487
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7574..8162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7654..7673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8333..9124,9125..9193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8956..8975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9194..9298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9366..9384
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    join(9412..9454,1..82)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
       61 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
      181 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      241 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      301 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      361 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
      421 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      481 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      541 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      601 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      661 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      721 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      781 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      841 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
      901 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
      961 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     1021 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     1081 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     1141 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     1201 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     1261 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     1321 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     1381 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     1441 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     1501 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     1561 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     1621 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgataaacg gccggccgag
     1681 ctcgcccggg gatcgagcgc agcggtataa aagggcgcgg ggtggctgag agcatcagtt
     1741 gtgaatgaat gttcgagccg agcagacgtg ccgctgcctt cgttaatatc ctttgaataa
     1801 gccaactttg aatcacaaga cgcataccaa acggtaccgc tagcagatcc gcccgccaag
     1861 cttgaagcaa gcctcaatca aaatgaagct gctgagtagt attgaacaag cgtgtgacat
     1921 atgccgactt aagaagctga agtgcagcaa ggaaaagccg aagtgcgcca aatgtcttaa
     1981 gaacaattgg gagtgccggt actcgccaaa gacgaagcga agccctttga cacgcgctca
     2041 tttgactgaa gtggagagtc gcctggagcg ccttgagcag ttgttcctcc tcatttttcc
     2101 ccgtgaagac ctcgacatga tactgaagat ggatagtctc caagatatta aggccctgct
     2161 cactggcctg tttgttcagg ataacgtcaa caaggatgcc gtgacggacc gccttgcctc
     2221 ggtggagaca gatatgccac ttaccttgcg gcagcatcgc atctcggcta ccagcagtag
     2281 tgaagaaagt tccaataagg gtcaacggca actgacggta tcgggatccg ccccaccgac
     2341 ggatgtgtcc ctcggagatg agttgcatct cgacggcgaa gacgttgcaa tggcccatgc
     2401 cgatgcgctg gacgatttcg atcttgacat gcttggcgat ggcgatagtc cgggaccggg
     2461 cttcactcct cacgattccg ccccatacgg agcgctcgat atggctgatt tcgagttcga
     2521 acagatgttt acagacgccc ttggcattga tgagtacggt ggataaaagc ttatcgatac
     2581 cgtcgactaa agccaaatag aaaattattc agttcctggc ttaagttttt aaaagtgata
     2641 ttatttattt ggttgtaacc aaccaaaaga atgtaaataa ctaatacata attatgttag
     2701 ttttaagtta gcaacaaatt gattttagct atattagcta cttggttaat aaatagaata
     2761 tatttattta aagataattg cgtttttatt gtcagggagt gagtttgctt aaaaactcgt
     2821 ttagatccac tagttctaga gcggccgctg gccacgggtg cgcatgatcg tgctcctgtc
     2881 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     2941 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     3001 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     3061 cattatgttc cggaggcgcg ccctagttcc agtgaaatcc aagcattttc taaattaaat
     3121 gtattcttat tattatagtt gttatttttg atatatataa acaacactat tatgcccacc
     3181 atttttttga gatgcatcta cacaaggaac aaacactgga tgtcactttc agttcaaatt
     3241 gtaacgctaa tcactccgaa caggtcacaa aaaattacct taaaaagtca taatattaaa
     3301 ttagaataaa tatagctgtg agggaaatat atacaaatat attggagcaa ataaattgta
     3361 catacaaata tttattacta atttctattg agacgaaatg aaccactcgg aaccatttga
     3421 gcgaaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa
     3481 tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg
     3541 aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg
     3601 tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg
     3661 gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc
     3721 tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc
     3781 aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga
     3841 tatatgtcgg ctactccttg cgtcgggccc gaagtcttag agccagatat gcgagcaccc
     3901 ggaagctcac gatgagaatg gccagaccca cgtagtccag cggcagatcg gcggcggaga
     3961 agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc
     4021 agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt
     4081 aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca
     4141 agaagaagcc gccaaagagc aggaatggta tgataaccgg cggacccaca gacagcgcca
     4201 tcgaggtcga ggagctggcg caggatatta gatatccgaa ggacgttgac acattggcca
     4261 ccagagtgac cagcgccagg cagttgaaga agtgcagcac tccggcccgc agtccgatca
     4321 tcggataggc aatcgccgtg aagaccagtg gcactgtgag aaaaagcggc aattcggcaa
     4381 tcgttttgcc cagaaagtat gtgtcacagc gataaagtcg acttcgggcc tccctcataa
     4441 aaactggcag ctctgaggtg aacacctaaa tcgaatcgat tcattagaaa gttagtaaat
     4501 tattgaaatg caaatgtatt ctaaacatga cttacattta tcgtggcaaa gacgttttga
     4561 aaggtcatgt tggtcaggaa gaggaagatg gctccgttga tattcatcac acccacttgc
     4621 gtgagttgtt ggcccaaaaa gatgaggcca atcaagatgg caaccatctg caaattaaaa
     4681 tgttactcgc atctcattaa tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa
     4741 ctataaacta tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctat
     4801 ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg
     4861 aaccactcac cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg
     4921 acagccagga tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt
     4981 acccattctc cggctgctcc agtggcttct ccagattttt ggtggccaac aactgctcca
     5041 tatcccgggc tactttgcta atggcaaaat tgtcgcatat cttggcgatc cgatcacggg
     5101 actcgatctc ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt
     5161 agttggtagg acactgggca cccacgctgg ataggagttg agatgttatg taatactaga
     5221 tacccttaat aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg
     5281 cccaagaaag ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc
     5341 tcggaagacg gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc
     5401 agcacctgga cgacgctgtg ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag
     5461 atcagaagcg gcggatcggt tagagcctcg gaggcgaatg ccagacgctt cctttctccg
     5521 ccggacagac ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc
     5581 gaaagctcct ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt
     5641 ggcatccgca ccatggcttg gaaaatcagg tgttccctgg ccgttaggga gccgataaag
     5701 aggtcatcct gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg
     5761 ccattgagca gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca
     5821 agggcattca gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg
     5881 cccggatagg ccacgccgca aactgagttt caaattggta attggaccct ttattaagat
     5941 ttcacacaga tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg
     6001 gcgccggtat gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc
     6061 atccggagcc cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga
     6121 ggttctcggc tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggccgga
     6181 gcgtgccgta gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc
     6241 cgctgtcacc ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct
     6301 aaccccgaac aaaaagtacc ccgaagtatc ctacgaagta ggtttatact tttatttatt
     6361 ttttgtgcat ctaggatcag cttaaaatat ctggttgtta tattttttgt aaaaaagaat
     6421 atagtcgaaa atgaatgcct ttagatgtct tgatcatgat atgatctcaa aaattgtctt
     6481 atatagcgag aacagctacc agaataatct gtttcgtgtc actatttgtt tgtgcaattg
     6541 cggtttggga tttttgtggg tcgcagttct cacgccgcag acaatttgat gttgcaatcg
     6601 cagttcctat agatcaagtg aacttaagat gtatgcacat gtactactca cattgttcag
     6661 atgctcggca gatgggtgtt tgctgcctcc gcgaattaat agctcctgat cctcttggcc
     6721 cattgccggg atttttcaca ctttcccctg cttacccacc caaaaccaat caccacccca
     6781 atcactcaaa aaacaaacaa aaataagaag cgagaggagt tttggcacag cactttgtgt
     6841 ttaattgatg gcgtaaaccg cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg
     6901 aaggcggaca ttgacgctac gtaacgctac aaacggtggc gaaagagata gcggacgcag
     6961 cggcgaaaga gacggcgata tttctgtgga cagagaagga ggcaaacagc gctgactttg
     7021 agtggaatgt cattttgagt gagaggtaat cgaaagaacc tggtacatca aatacccttg
     7081 gatcgaagta aatttaaaac tgatcagata agttcaatga tatccagtgc agtaaaaaaa
     7141 aaaaatgttt tttttatcta ctttccgcaa aaatgggttt tattaactta catacatact
     7201 aggcgcgccc atatgttcgg cttgtcgaca tgcccgccgt gaccgtcgag aacccgctga
     7261 cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca
     7321 ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca
     7381 actaccgcca cctcgacccg ttcatcatga tggaccagat gggtgaggtg gagtacgcgc
     7441 ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca
     7501 tcgtcgacgg taacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8221 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8281 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8341 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8401 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8461 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8521 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8581 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8641 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8701 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8761 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8821 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8881 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8941 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9001 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9061 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9121 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9181 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9301 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9361 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcgtttat cacaagtttg
     9421 tacaaaaaag ctgaacgaga aacgtaaaat gata
//
