LOCUS       sequence-246144-       10252 bp    DNA     circular SYN 15-APR-2025
DEFINITION  express mutant BAP1-E31A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X, 
            Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P, 
            Li W, Gan B
  TITLE     BAP1 links metabolic regulation of ferroptosis to tumour 
            suppression.
  JOURNAL   Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 
            10.1038/s41556-018-0178-0. Epub 2018 Sep 10.
  PUBMED    30202049
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0178-0"; journalName: "Nat Cell Biol"; date: 
            "2018-10"; volume: "20"; issue: "10"; pages: "1181-1192"
COMMENT     Sequence Label: pLVX-M-Flag-BAP1-E31A
FEATURES             Location/Qualifiers
     source          1..10252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2840..2863
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4055..4066
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        5087..5586
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5122..5145)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5519..5538
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5607..6206
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5607..5626)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6103..6123
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6220..6808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6273..6293)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6691..6702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7015..7648
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7777..7793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7777..7793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7790..7812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7801..7817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7825..7831,7832..7849,7850..7855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7870..7891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8008..8025)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8179..8767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8259..8278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8938..9729,9730..9798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9561..9580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9799..9903)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9951..10085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9988..10007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10042..10061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcatcggga tccaggatgg actacaaaga cgatgacgac aagctcgaga ataagggctg
     2881 gctggagctg gagagcgacc caggcctctt caccctgctc gtggaagatt tcggtgtcaa
     2941 gggggtgcaa gtggaggcga tctacgacct tcagagcaaa tgtcagggcc ctgtatatgg
     3001 atttatcttc ctgttcaaat ggatcgaaga gcgccggtcc cggcgaaagg tctctacctt
     3061 ggtggatgat acgtccgtga ttgatgatga tattgtgaat aacatgttct ttgcccacca
     3121 gctgataccc aactcttgtg caactcatgc cttgctgagc gtgctcctga actgcagcag
     3181 cgtggacctg ggacccaccc tgagtcgcat gaaggacttc accaagggtt ttagccctga
     3241 gagcaaagga tatgcgattg gcaatgcccc ggagttggcc aaggcccata atagccatgc
     3301 caggcccgag ccacgccacc tccctgagaa gcagaatggc cttagtgcag tgcggaccat
     3361 ggaggcgttc cactttgtca gctatgtgcc tatcacaggc cggctctttg agctggatgg
     3421 gctgaaggtc taccccattg accatgggcc ctggggggag gacgaggagt ggacagacaa
     3481 ggcccggcgg gtcatcatgg agcgtatcgg cctcgccact gcaggggagc cctaccacga
     3541 catccgcttc aacctgatgg cagtggtgcc cgaccgcagg atcaagtatg aggccaggct
     3601 gcatgtgctg aaggtgaacc gtcagacagt actagaggct ctgcagcagc tgataagagt
     3661 aacacagcca gagctgattc agacccacaa gtctcaagag tcacagctgc ctgaggagtc
     3721 caagtcagcc agcaacaagt ccccgctggt gctggaagca aacagggccc ctgcagcctc
     3781 tgagggcaac cacacagatg gtgcagagga ggcggctggt tcatgcgcac aagccccatc
     3841 ccacagccct cccaacaaac ccaagctagt ggtgaagcct ccaggcagca gcctcaatgg
     3901 ggttcacccc aaccccactc ccattgtcca gcggctgccg gcctttctag acaatcacaa
     3961 ttatgccaag tcccccatgc aggaggaaga agacctggcg gcaggtgtgg gccgcagccg
     4021 agttccagtc cgcccacccc agcagtactc agatgatgag gatgactatg aggatgacga
     4081 ggaggatgac gtgcagaaca ccaactctgc ccttaggtat aaggggaagg gaacagggaa
     4141 gccaggggca ttgagcggtt ctgctgatgg gcaactgtca gtgctgcagc ccaacaccat
     4201 caacgtcttg gctgagaagc tcaaagagtc ccagaaggac ctctcaattc ctctgtccat
     4261 caagactagc agcggggctg ggagtccggc tgtggcagtg cccacacact cgcagccctc
     4321 acccaccccc agcaatgaga gtacagacac ggcctctgag atcggcagtg ctttcaactc
     4381 gccactgcgc tcgcctatcc gctcagccaa cccgacgcgg ccctccagcc ctgtcacctc
     4441 ccacatctcc aaggtgcttt ttggagagga tgacagcctg ctgcgtgttg actgcatacg
     4501 ctacaaccgt gctgtccgtg atctgggtcc tgtcatcagc acaggcctgc tgcacctggc
     4561 tgaggatggg gtgctgagtc ccctggcgct gacagagggt gggaagggtt cctcgccctc
     4621 catcagacca atccaaggca gccaggggtc cagcagccca gtggagaagg aggtcgtgga
     4681 agccacggac agcagagaga agacggggat ggtgaggcct ggcgagccct tgagtgggga
     4741 gaaatactca cccaaggagc tgctggcact gctgaagtgt gtggaggctg agattgcaaa
     4801 ctatgaggcg tgcctcaagg aggaggtaga gaagaggaag aagttcaaga ttgatgacca
     4861 gagaaggacc cacaactacg atgagttcat ctgcaccttt atctccatgc tggctcagga
     4921 aggcatgctg gccaacctag tggagcagaa catctccgtg cggcggcgcc aaggggtcag
     4981 catcggccgg ctccacaagc agcggaagcc tgaccggcgg aaacgctctc gcccctacaa
     5041 ggccaagcgc cagtgagaat tccgacatgc tctagataat tctaccgggt aggggaggcg
     5101 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     5161 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     5221 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5281 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     5341 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     5401 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     5461 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     5521 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     5581 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     5641 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5701 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5761 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5821 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5881 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5941 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6001 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6061 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6121 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6181 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa
     6241 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6301 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6361 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6421 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6481 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6541 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6601 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6661 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6721 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6781 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     6841 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6901 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6961 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     7021 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7081 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     7141 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7201 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7261 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7321 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     7381 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7441 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7501 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7561 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7621 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7681 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     7741 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9901 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
     9961 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10021 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10081 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    10141 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10201 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
