LOCUS       sequence-246305-       15013 bp    DNA     circular SYN 15-APR-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15013)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX015
FEATURES             Location/Qualifiers
     source          1..15013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5127..5151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5180..5269
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5303..5555
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5984..6236)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6704..7729)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6704..7729)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7739..8083)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8225..8242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8225..8241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8234..8256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8381..8400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8591..8612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8859..8883
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10479..11627)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11926..12720)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12912..13679
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14372..15003
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggagat
     4621 gggtggtggg ggtcaaagtg gaggagttgc aagttcaggg gggtgttgcg gcgctaagag
     4681 aaaaaacgaa aggccttaca aaggaataag gatgaggaag tggggaaagt gggttgcaga
     4741 aattagagag cccaataagc gttcgagaat ttggttgggc tcgtattcaa cgccggtggc
     4801 ggcggcgcgg gcttacgata cggcggtgta ttatttacgc gggccttcgg cccggttgaa
     4861 ttttcctgag ttgttgggtg gtgatggtgg gcttaatgat ttatctgctg cctcaattcg
     4921 taaaaaggcc atagaagttg gtgcccaagt tgatgcgata cagaactcac ttgcaactca
     4981 tcacggccac gaccatcgag aagaaaaagt gcactgtgag actgcaagcc cgtctgaatt
     5041 gaagccatgt tggtttcagg agaagcccga tttaaacaag aagcccgagc ccgaagaccc
     5101 agaacaggat tactgtggtc ccggggaccc agctttcttg tacaaagtgg ttgataacag
     5161 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     5221 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
     5281 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     5341 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     5401 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     5461 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     5521 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga
     5581 catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct
     5641 tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg
     5701 tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc
     5761 atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc
     5821 aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga
     5881 aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc
     5941 ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat
     6001 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     6061 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     6121 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     6181 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     6241 tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa
     6301 aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat
     6361 taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga
     6421 ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat
     6481 agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc
     6541 agtattccct caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag
     6601 aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt
     6661 ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg
     6721 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     6781 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     6841 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     6901 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     6961 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     7021 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     7081 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     7141 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     7201 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     7261 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     7321 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     7381 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     7441 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     7501 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     7561 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     7621 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     7681 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt
     7741 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     7801 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     7861 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     7921 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     7981 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     8041 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     8101 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac
     8161 ggatctctag gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc ctagaagcta
     8221 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8281 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8341 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
     8401 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8461 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     8521 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8581 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     8641 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
     8701 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     8761 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
     8821 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     8881 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     8941 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     9001 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     9061 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     9121 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
     9181 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
     9241 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
     9301 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
     9361 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
     9421 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
     9481 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
     9541 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
     9601 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
     9661 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
     9721 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
     9781 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
     9841 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
     9901 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
     9961 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    10021 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    10081 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    10141 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    10201 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    10261 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    10321 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    10381 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    10441 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    10501 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    10561 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    10621 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    10681 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    10741 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    10801 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    10861 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    10921 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    10981 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    11041 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    11101 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    11161 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    11221 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    11281 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    11341 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    11401 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    11461 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    11521 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    11581 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    11641 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    11701 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    11761 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    11821 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    11881 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    11941 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    12001 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    12061 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    12121 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    12181 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    12241 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    12301 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    12361 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    12421 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    12481 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    12541 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    12601 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    12661 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    12721 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    12781 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    12841 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    12901 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    12961 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    13021 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    13081 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    13141 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    13201 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    13261 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    13321 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    13381 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    13441 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    13501 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    13561 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    13621 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    13681 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    13741 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    13801 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    13861 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    13921 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    13981 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    14041 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    14101 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    14161 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    14221 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    14281 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    14341 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    14401 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    14461 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    14521 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    14581 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    14641 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    14701 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    14761 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    14821 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    14881 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    14941 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    15001 gtcggaaagg cgc
//
