LOCUS       sequence-246584-       14948 bp    DNA     circular SYN 15-APR-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14948)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 14948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX052
FEATURES             Location/Qualifiers
     source          1..14948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5094..5118)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5147..5236
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5270..5522
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(5951..6203)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6671..7696)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6671..7696)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7706..8050)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8160..8177)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8160..8176)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8169..8191)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8316..8335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8526..8547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8794..8818
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10414..11562)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11861..12655)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12847..13614
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14307..14938
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgtwccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgcaaga
     4621 acaagcgacc agttccattg ctgctagttc tataccttca agtagtgaga gatcttctag
     4681 ctctgctctc cattttgaaa tcaaagaagg tatggagagt gatgatgaga tcagaagagt
     4741 gccggagatg ggcggagaag ccaccggaac cacctcagct tctggtagag atggcacttc
     4801 cgccaccggt caagctcagc cttcagctgg tactcaaagg aagagaggaa gaagtccagc
     4861 tgacaaagaa aacaagaggc taaaaaggtt gttgagaaat agagtgtcag cacagcaagc
     4921 aagggaaagg aagaaagcat acttgataga tctagaagca agggtgaaag aattggaaac
     4981 aaagaatgca gaacttgaag agaggttatc cactttgcaa aatgagaacc agatgctcag
     5041 acatatattg aagaacacaa cagcaggtgc acaagaaggg aggaaacccg gggacccagc
     5101 tttcttgtac aaagtggttg ataacagcgg gttaattaac atcttttacc catacgatgt
     5161 tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga
     5221 cgttccagat tacgctgctc agtgcagcgc ttagagctcg aatttccccg atcgttcaaa
     5281 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5341 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5401 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5461 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5521 tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg agaggaattt
     5581 ccgacgagca cagaaaggac ttgctcttgg acgtaggcct atttctcagg cacatgtatc
     5641 aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc gccaactggg agatgaggag
     5701 gctttcttgg ggggcagtca gcagttcatt tcacaagaca gaggaacttg taaggagatg
     5761 cactgattta tcttggcgca aaccagcagg acgaattagt gggaatagcc cgcgaatatc
     5821 taagttatgc ctgtcggcat gagcagaaac ttccaattcg aaacagtttg gagaggttgt
     5881 ttttgggcat accttttgtt agtcagcctc tcgattgctc atcgtcatta cacagtaccg
     5941 aagtttgatc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     6001 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     6061 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     6121 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     6181 tattgccaaa tgtttgaacg atctgcttcg acgcactcct tctttactcc accatctcgt
     6241 ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc gctggaactg aagttaccaa
     6301 tcacgctgga tgatttgcca gttggattaa tcttgccttt ccccgcatga ataatattga
     6361 tgaatgcatg cgtgaggggt atttcgattt tggcaatagc tgcaattgcc gcgacatcct
     6421 ccaacgagca taattcttca gaaaaatagc gatgttccat gttgtcaggg catgcatgat
     6481 gcacgttatg aggtgacggt gctaggcagt attccctcaa agtttcatag tcagtatcat
     6541 attcatcatt gcattcctgc aagagagaat tgagacgcaa tccacacgct gcggcaacct
     6601 tccggcgttc gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg gatcccggtc
     6661 ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     6721 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     6781 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     6841 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     6901 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     6961 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     7021 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     7081 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     7141 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     7201 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     7261 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     7321 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     7381 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     7441 tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     7501 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     7561 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     7621 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     7681 gttcaggctt tttcatatcg gggtcgtcct ctccaaatga aatgaacttc cttatataga
     7741 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
     7801 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     7861 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     7921 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     7981 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     8041 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     8101 gagagtgtcg tgctccacca tgttgacgga tctctaggac gcgtcctaga agctaattca
     8161 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8221 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8281 ccttcccaac agttgcgcag cctgaatggc gcccgctcct ttcgctttct tcccttcctt
     8341 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8401 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     8461 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8521 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     8581 tgatttataa gggattttgc cgatttcgga accaccatca aacaggattt tcgcctgctg
     8641 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     8701 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta aaaacgtccg
     8761 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     8821 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     8881 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     8941 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     9001 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaaata
     9061 tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg cttaaccgtg
     9121 acaggctgtc gatcttgaga actatgccga cataatagga aatcgctgga taaagccgct
     9181 gaggaagctg agtggcgcta tttctttaga agtgaacgtt gacgatatca actcccctat
     9241 ccattgctca ccgaatggta caggtcgggg acccgaagtt ccgactgtcg gcctgatgca
     9301 tccccggctg atcgacccca gatctggggc tgagaaagcc cagtaaggaa acaactgtag
     9361 gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc
     9421 cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca
     9481 ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga
     9541 gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg
     9601 cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca
     9661 acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc
     9721 tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg
     9781 ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa
     9841 tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga
     9901 cgatcatcac gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc
     9961 ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg
    10021 ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga
    10081 atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct
    10141 cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgcgga
    10201 aatcctgcac gtcggccgct ccaagccgtc gaatctgagc cttaatcaca attgtcaatt
    10261 ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc
    10321 gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc
    10381 tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc
    10441 attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac
    10501 ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc
    10561 cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt
    10621 cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag
    10681 cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag
    10741 gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa
    10801 gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc
    10861 attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc
    10921 gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc
    10981 ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt
    11041 gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc
    11101 cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag
    11161 ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct
    11221 gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg
    11281 cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg
    11341 ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc
    11401 gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc
    11461 acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat
    11521 cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat
    11581 tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt
    11641 ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc
    11701 cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa
    11761 atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg
    11821 ctcctgcttg tcgccggcat cgttgcgcca catctaggta ctaaaacaat tcatccagta
    11881 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
    11941 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
    12001 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
    12061 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
    12121 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
    12181 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
    12241 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
    12301 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
    12361 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
    12421 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
    12481 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
    12541 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
    12601 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
    12661 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    12721 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    12781 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca
    12841 attatgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
    12901 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
    12961 cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca
    13021 actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc
    13081 catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat
    13141 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt
    13201 gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc
    13261 cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc
    13321 ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg
    13381 gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac
    13441 gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac
    13501 ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa
    13561 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat tacccaatta
    13621 tgatagaatt tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag
    13681 tttttatgct ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc
    13741 ctgaaatcct tacatacggc gatatcttct atataaaaga tatattatct tatcagtatt
    13801 gtcaatatat tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt cttggtagct
    13861 ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc caattctcgt tttcatacct
    13921 cggtataatc ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc cccgaacacg
    13981 agcacggcac ccgcgaccac tatgccaaga atgcccaagg taaaaattgc cggccccgcc
    14041 atgaagtccg tgaatgcccc gacggccgaa gtgaagggca ggccgccacc caggccgccg
    14101 ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg
    14161 cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat cccggcaatg
    14221 gcaaggactg ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc
    14281 ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc
    14341 ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat
    14401 aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa
    14461 acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc
    14521 ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt
    14581 agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat
    14641 ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca
    14701 cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc
    14761 ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta
    14821 tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
    14881 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg
    14941 aaaggcgc
//
