LOCUS       sequence-246599-       15550 bp    DNA     circular SYN 15-APR-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15550)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX059
FEATURES             Location/Qualifiers
     source          1..15550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5664..5688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5717..5806
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5840..6092
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6521..6773)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7241..8266)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7241..8266)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8276..8620)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8762..8779)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8762..8778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8771..8793)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8918..8937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9128..9149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9396..9420
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11016..12164)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12463..13257)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLQGIKPDWEKIKYYILLDELF"
     mobile_element  13449..14216
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14909..15540
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg gttttttcct ctatttcagc
     4621 ttatcttgat ccagccaact ggcaacagca agctggacat agcatcccga atccgcagtt
     4681 ccaatcagtg caaccgctgc ggccactagc tccaccacca gttcagactc atgtgggcgg
     4741 tggaagcacc attaggccgg gctcgatggc tgaccgagcc cggttagcca acataccaat
     4801 ggccgaggcg gtccaaaaat gccctcgttg tgaatcaaca aacaccaagt tttgctactt
     4861 caacaattat agtctttcgc agcctcgtca tttctgcaag acctgcagaa ggtactggac
     4921 aagagggggc gctctgagga gcgtccctgt gggtggtggt tgtcggagga acaaaaggag
     4981 cagcagtagt actaaagcaa ccaataacaa taataatact cctaaatctc cagcttctag
     5041 cactagtact ggtggcggcc gccaaatacc tactaattct ggttcaacaa acaacaataa
     5101 tagtttctct agtccatcat ctgcagcaag tttactaggt cttatgtccc ctcaaattca
     5161 ttaccctcta cgtttcatgt ctcctttagg tcaattaact gatcatcact tcactccaaa
     5221 tgacaataat attaccatga attactcggc aatttcctca tcatcagctc cagttacggg
     5281 aaccaacgaa agcatgaatt ttcagcttgc aatgggtggt aatctagagc agtggcggct
     5341 gcaacaacaa gtggccaacc aattccctaa tttctacggg ggattggact cgtcttcagc
     5401 ttctgggctt tatccttttt accccgctta ctatacaagt aatgacggtg ttactgtaac
     5461 aagtagtcag attaggccaa aaatcacgaa tcccatgctc acacagctgg ctttgatgaa
     5521 gatggaagac aatcacgggc attcaagtct gtcgcggcag tttgtaggga atcaaggcag
     5581 tgagaactgg agtaatggta atgcttggaa tgagctttcg gcgagtttta gctcttctcc
     5641 taccagtaat gcccttcccg gggacccagc tttcttgtac aaagtggttg ataacagcgg
     5701 gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     5761 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc
     5821 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     5881 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     5941 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     6001 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     6061 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     6121 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     6181 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     6241 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     6301 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     6361 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     6421 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     6481 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     6541 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     6601 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     6661 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     6721 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     6781 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     6841 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     6901 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     6961 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     7021 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     7081 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     7141 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     7201 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     7261 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     7321 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     7381 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     7441 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     7501 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     7561 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     7621 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     7681 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     7741 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     7801 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     7861 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     7921 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7981 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     8041 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     8101 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     8161 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     8221 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     8281 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     8341 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8401 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8461 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     8521 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     8581 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     8641 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     8701 tctctaggac gcgtcgacgc gtcgacgcgt cgacgcgtcg acgcgtccta gaagctaatt
     8761 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8821 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8881 gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc
     8941 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9001 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     9061 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9121 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     9181 tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat tttcgcctgc
     9241 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     9301 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc
     9361 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     9421 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     9481 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     9541 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     9601 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa
     9661 tatcatctcc ctcgcagaga tccgaattat cagccttctt attcatttct cgcttaaccg
     9721 tgacaggctg tcgatcttga gaactatgcc gacataatag gaaatcgctg gataaagccg
     9781 ctgaggaagc tgagtggcgc tatttcttta gaagtgaacg ttgacgatat caactcccct
     9841 atccattgct caccgaatgg tacaggtcgg ggacccgaag ttccgactgt cggcctgatg
     9901 catccccggc tgatcgaccc cagatctggg gctgagaaag cccagtaagg aaacaactgt
     9961 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
    10021 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa
    10081 cactaaagct actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt
    10141 gagcagaggc acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac
    10201 cgcccccgcc aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac
    10261 caacagcgcc acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg
    10321 gctacctagc agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt
    10381 cgccgcgacc ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga
    10441 aatattaagt gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg
    10501 gacgatcatc acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc
    10561 tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt
    10621 ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc
    10681 gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg
    10741 ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg
    10801 gaaatcctgc acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa
    10861 ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga
    10921 gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct
    10981 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
    11041 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
    11101 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
    11161 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
    11221 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
    11281 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
    11341 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
    11401 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
    11461 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
    11521 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
    11581 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
    11641 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
    11701 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
    11761 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
    11821 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
    11881 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
    11941 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
    12001 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
    12061 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
    12121 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
    12181 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
    12241 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
    12301 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
    12361 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
    12421 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
    12481 taaaatataa tattttattt tctcccaatc aggcttgatc ccctgtaagt caaaaaatag
    12541 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    12601 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    12661 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    12721 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    12781 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    12841 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    12901 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    12961 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    13021 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    13081 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    13141 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    13201 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    13261 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    13321 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    13381 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
    13441 caattatggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
    13501 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
    13561 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
    13621 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
    13681 gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg
    13741 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
    13801 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc
    13861 gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat
    13921 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
    13981 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
    14041 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
    14101 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
    14161 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccaat
    14221 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
    14281 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
    14341 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta
    14401 ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag
    14461 ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac
    14521 ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca
    14581 cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg
    14641 ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca cccaggccgc
    14701 cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa
    14761 tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg atcccggcaa
    14821 tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
    14881 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
    14941 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
    15001 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
    15061 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
    15121 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
    15181 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
    15241 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
    15301 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
    15361 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
    15421 tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
    15481 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc
    15541 ggaaaggcgc
//
