LOCUS       sequence-246613-        9327 bp    DNA     circular SYN 14-APR-2025
DEFINITION  doxycycline-inducible expression of human GATA2 (3xFLAG tagged) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Gomes AM, Kurochkin I, Chang B, Daniel M, Law K, Satija N, Lachmann 
            A, Wang Z, Ferreira L, Ma'ayan A, Chen BK, Papatsenko D, Lemischka 
            IR, Moore KA, Pereira CF
  TITLE     Cooperative Transcription Factor Induction Mediates Hemogenic 
            Reprogramming.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. doi: 
            10.1016/j.celrep.2018.11.032.
  PUBMED    30517869
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-4- 4"; volume: "25"; issue: "10"; pages: "2821-2835.e7"
COMMENT     Sequence Label: pFUW-tetO-3xFLAG-GATA2
FEATURES             Location/Qualifiers
     source          1..9327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..126
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    619..852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1037..1081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1379..1496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1553..1571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1595..1613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1637..1655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1679..1697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1721..1739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1763..1781
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1814..1852
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1849..1873
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2005..2024
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2075..2099
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2108..2173
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     LTR             4071..4251
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4277..4294)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4283..4507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4553..4981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4640..4659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4850..4871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4990..5010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4995..5324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5175..5310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5372..5419
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5438..5812
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5942..6063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5979..5998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6033..6052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6112..6128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6112..6128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6125..6147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6136..6152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6160..6166,6167..6184,6185..6190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6205..6226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6343..6360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6514..7102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6594..6613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7273..8064,8065..8133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7896..7915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8134..8238)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8313..8332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8504..8883
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8885..9083
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9101..9281
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
       61 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      121 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      181 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      241 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
      301 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
      361 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
      421 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
      481 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
      541 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
      601 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
      661 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      721 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      781 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      841 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      901 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      961 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1021 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1081 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1141 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1201 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     1261 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     1321 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     1381 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1441 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     1501 tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc
     1561 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     1621 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     1681 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     1741 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg
     1801 gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
     1861 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     1921 ggaccgatcc agcctccgcg gccccgaatt ctagagcctc tgctaaccat gttcatgcct
     1981 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     2041 gcaaagaatt cctcgacggt atcgataagc tatcacaagt ttgtacaaaa aagcaggctt
     2101 caccatggac tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga
     2161 tgacgatgac aagggatccg gcggcggcgg cggcgccgct agcatggagg tggcgcccga
     2221 gcagccgcgc tggatggcgc acccggccgt gctgaatgcg cagcaccccg actcacacca
     2281 cccgggcctg gcgcacaact acatggaacc cgcgcagctg ctgcctccag acgaggtgga
     2341 cgtcttcttc aatcacctcg actcgcaggg caacccctac tatgccaacc ccgctcacgc
     2401 gcgggcgcgc gtctcctaca gccccgcgca cgcccgcctg accggaggcc agatgtgccg
     2461 cccacacttg ttgcacagcc cgggtttgcc ctggctggac gggggcaaag cagccctctc
     2521 tgccgctgcg gcccaccacc acaacccctg gaccgtgagc cccttctcca agacgccact
     2581 gcacccctca gctgctggag gccctggagg cccactctct gtgtacccag gggctggggg
     2641 tgggagcggg ggaggcagcg ggagctcagt ggcctccctc acccctacag cagcccactc
     2701 tggctcccac cttttcggct tcccacccac gccacccaaa gaagtgtctc ctgaccctag
     2761 caccacgggg gctgcgtctc cagcctcatc ttccgcgggg ggtagtgcag cccgaggaga
     2821 ggacaaggac ggcgtcaagt accaggtgtc actgacggag agcatgaaga tggaaagtgg
     2881 cagtcccctg cgcccaggcc tagctactat gggcacccag cctgctacac accaccccat
     2941 ccccacctac ccctcctatg tgccggcggc tgcccacgac tacagcagcg gactcttcca
     3001 ccccggaggc ttcctggggg gaccggcctc cagcttcacc cctaagcagc gcagcaaggc
     3061 tcgttcctgt tcagaaggcc gggagtgtgt caactgtggg gccacagcca cccctctctg
     3121 gcggcgggac ggcaccggcc actacctgtg caatgcctgt ggcctctacc acaagatgaa
     3181 tgggcagaac cgaccactca tcaagcccaa gcgaagactg tcggccgcca gaagagccgg
     3241 cacctgttgt gcaaattgtc agacgacaac caccacctta tggcgccgaa acgccaacgg
     3301 ggaccctgtc tgcaacgcct gtggcctcta ctacaagctg cacaatgtta acaggccact
     3361 gaccatgaag aaggaaggga tccagactcg gaaccggaag atgtccaaca agtccaagaa
     3421 gagcaagaaa ggggcggagt gcttcgagga gctgtcaaag tgcatgcagg agaagtcatc
     3481 ccccttcagt gcagctgccc tggctggaca catggcacct gtgggccacc tcccgccctt
     3541 cagccactcc ggacacatcc tgcccactcc gacgcccatc cacccctcct ccagcctctc
     3601 cttcggccac ccccacccgt ccagcatggt gaccgccatg ggctagtgat taggaggtgg
     3661 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3721 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3781 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     3841 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     3901 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     3961 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     4021 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     4081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     4261 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4321 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4381 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4441 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4501 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     4561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4981 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5041 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5161 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5221 gctgactaat tttttttatt tatgcaaagg ccgaggccgc ctctgcctct gagctattcc
     5281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5341 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     5401 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     5461 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     5521 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     5581 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     5641 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     5701 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     5761 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     5821 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5881 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5941 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6061 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6241 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6301 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7201 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7261 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7321 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7381 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7441 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7501 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7561 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7621 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7681 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7741 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7801 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7861 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7921 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7981 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8041 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8101 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8161 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8221 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     8281 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8341 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8401 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     8461 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     8521 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     8581 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8641 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8701 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     8761 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     8821 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     8881 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     8941 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     9001 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     9061 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     9121 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9181 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9241 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     9301 ttgaaagcga aagggaaacc agaggag
//
