LOCUS       sequence-246992-        7131 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Eukaryotic expression of FYN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Haruki H, Gonzalez MR, Johnsson K
  TITLE     Exploiting ligand-protein conjugates to monitor ligand-receptor 
            interactions.
  JOURNAL   PLoS One. 2012;7(5):e37598. doi: 10.1371/journal.pone.0037598. Epub 
            2012 May 31.
  PUBMED    22701522
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(5):e37598. doi"; pages: " 
            10.1371/journal.pone.0037598. Epub 2012 May 31"
COMMENT     Sequence Label: pCDNA3.1-V5-FYN
FEATURES             Location/Qualifiers
     source          1..7131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             914..955
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             956..976
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    1007..1031
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2679..2703)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2722..2739)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2728..2952
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2998..3426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3085..3104)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3295..3316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3435..3455)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3440..3769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3620..3755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3682..3701
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3836..4630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3890..3909)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4500..4519
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4804..4925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4841..4860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4895..4914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4974..4990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4974..4990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4987..5009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4998..5014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5022..5028,5029..5046,5047..5052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5067..5088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5205..5222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5376..5964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5456..5475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6135..6926,6927..6995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6758..6777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6996..7100)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttacc atgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacggaaaa
      961 cctgtatttt cagggcccgg atccgtcgac gaattctgca gatatcacaa gtttgtacaa
     1021 aaaagcaggc ttggaaggag ttcgaaccat gggctgtgtg caatgtaagg ataaagaagc
     1081 aacaaaactg acggaggaga gggacggcag cctgaaccag agctctgggt accgctatgg
     1141 cacagacccc acccctcagc actaccccag cttcggtgtg acctccatcc ccaactacaa
     1201 caacttccac gcagccgggg gccaaggact caccgtcttt ggaggtgtga actcttcgtc
     1261 tcatacgggg accttgcgta cgagaggagg aacaggagtg acactctttg tggcccttta
     1321 tgactatgaa gcacggacag aagatgacct gagttttcac aaaggagaaa aatttcaaat
     1381 attgaacagc tcggaaggag attggtggga agcccgctcc ttgacaactg gagagacagg
     1441 ttacattccc agcaattatg tggctccagt tgactctatc caggcagaag agtggtactt
     1501 tggaaaactt ggccgaaaag atgctgagcg acagctattg tcctttggaa acccaagagg
     1561 tacctttctt atccgcgaga gtgaaaccac caaaggtgcc tattcacttt ctatccgtga
     1621 ttgggatgat atgaaaggag accatgtcaa acattataaa attcgcaaac ttgacaatgg
     1681 tggatactac attaccaccc gggcccagtt tgaaacactt cagcagcttg tacaacatta
     1741 ctcagagaga gctgcaggtc tctgctgccg cctagtagtt ccctgtcaca aagggatgcc
     1801 aaggcttacc gatctgtctg tcaaaaccaa agatgtctgg gaaatccctc gagaatccct
     1861 gcagttgatc aagagactgg gaaatgggca gtttggggaa gtatggatgg gtacctggaa
     1921 tggaaacaca aaagtagcca taaagactct taaaccaggc acaatgtccc ccgaatcatt
     1981 ccttgaggaa gcgcagatca tgaagaagct gaagcacgac aagctggtcc agctctatgc
     2041 agtggtgtct gaggagccca tctacatcgt caccgagtat atgaacaaag gaagtttact
     2101 ggatttctta aaagatggag aaggaagagc tctgaaatta ccaaatcttg tggacatggc
     2161 agcacaggtg gctgcaggaa tggcttacat cgagcgcatg aattatatcc atagagatct
     2221 gcgatcagca aacattctag tggggaatgg actcatatgc aagattgctg acttcggatt
     2281 ggcccgattg atagaagaca atgagtacac agcaagacaa ggtgcaaagt tccccatcaa
     2341 gtggacggcc cccgaggcag ccctgtacgg gaggttcaca atcaagtctg acgtgtggtc
     2401 ttttggaatc ttactcacag agctggtcac caaaggaaga gtgccatacc caggcatgaa
     2461 caaccgggag gtgctggagc aggtggagcg aggctacagg atgccctgcc cgcaggactg
     2521 ccccatctct ctgcatgagc tcatgatcca ctgctggaaa aaggaccctg aagaacgccc
     2581 cacttttgag tacttgcaga gcttcctgga agactacttt accgcgacag agccccagta
     2641 ccaacctggt gaaaacctgt agctcgcgtg cggccgcaac ccagctttct tgtacaaagt
     2701 ggtgataaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2761 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2821 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2881 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2941 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     3001 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3061 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3121 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3181 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3241 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3301 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3361 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3421 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3541 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3601 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3661 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     3721 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     3781 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     3841 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3901 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3961 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4021 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4081 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4141 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4201 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4261 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4321 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4381 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4441 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4501 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4561 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4621 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4681 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4741 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4801 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4921 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     4981 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5041 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5101 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5161 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5221 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5281 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5341 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5401 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5461 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5521 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5581 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5641 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5701 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5761 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5821 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5881 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5941 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6001 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6061 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6121 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6181 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6241 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6301 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6361 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6421 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6481 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6541 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6601 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6661 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6721 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6781 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6841 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6901 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6961 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7021 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7081 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
