LOCUS       sequence-247016-        1211 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Fluoride riboswitch-regulated catechol 2,3-dioxygenase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Thavarajah W, Silverman AD, Verosloff M, Kelley-Loughnane N, Jewett 
            MC, Lucks JB
  TITLE     Point-of-Use Detection of Environmental Fluoride via a Cell-Free 
            Riboswitch-Based Biosensor.
  JOURNAL   ACS Synth Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347.
  PUBMED    31829623
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347."
COMMENT     Sequence Label: pJBL7025
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     terminator      1091..1162
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1178..1205
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgacagcta gctcagtcct aggtataata ctagtttata ggcgatggag ttcgccataa
       61 acgctgctta gctaatgact cctaccagta tcactactgg taggagtcta tttttttagg
      121 aggaaggatc tatgaacaaa ggtgtaatgc gaccgggcca tgtgcagctg cgtgtactgg
      181 acatgagcaa ggccctggaa cactacgtcg agttgctggg cctgatcgag atggaccgtg
      241 acgaccaggg ccgtgtctat ctgaaggctt ggaccgaagt ggataagttt tccctggtgc
      301 tacgcgaggc tgacgagccg ggcatggatt ttatgggttt caaggttgtg gatgaggatg
      361 ctctccggca actggagcgg gatctgatgg catatggctg tgccgttgag cagctacccg
      421 caggtgaact gaacagttgt ggccggcgcg tgcgcttcca ggccccctcc gggcatcact
      481 tcgagttgta tgcagacaag gaatatactg gaaagtgggg tttgaatgac gtcaatcccg
      541 aggcatggcc gcgcgatctg aaaggtatgg cggctgtgcg tttcgaccac gccctcatgt
      601 atggcgacga attgccggcg acctatgacc tgttcaccaa ggtgctcggt ttctatctgg
      661 ccgaacaggt gctggacgaa aatggcacgc gcgtcgccca gtttctcagt ctgtcgacca
      721 aggcccacga cgtggccttc attcaccatc cggaaaaagg ccgcctccat catgtgtcct
      781 tccacctcga aacctgggaa gacttgcttc gcgccgccga cctgatctcc atgaccgaca
      841 catctatcga tatcggccca acccgccacg gcctcactca cggcaagacc atctacttct
      901 tcgacccgtc cggtaaccgc aacgaagtgt tctgcggggg agattacaac tacccggacc
      961 acaaaccggt gacctggacc accgaccagc tgggcaaggc gatcttttac cacgaccgca
     1021 ttctcaacga acgattcatg accgtgctga cctgataagg atccaaactc gagtaaggat
     1081 ctccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1141 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1201 ctgcgtttat a
//
