LOCUS       sequence-247017-         398 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Fluoride riboswitch-regulated 3-way junction dimeric broccoli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Thavarajah W, Silverman AD, Verosloff M, Kelley-Loughnane N, Jewett 
            MC, Lucks JB
  TITLE     Point-of-Use Detection of Environmental Fluoride via a Cell-Free 
            Riboswitch-Based Biosensor.
  JOURNAL   ACS Synth Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347.
  PUBMED    31829623
REFERENCE   2  (bases 1 to 398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347."
COMMENT     Sequence Label: pJBL7026
FEATURES             Location/Qualifiers
     source          1..398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     terminator      278..349
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      365..392
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgacagcta gctcagtcct aggtataata ctagtttata ggcgatggag ttcgccataa
       61 acgctgctta gctaatgact cctaccagta tcactactgg taggagtcta tttttttccc
      121 acatactctg atgatccgag acggtcgggt ccagatattc gtatctgtcg agtagagtgt
      181 gggctcggat cattcatggc aagagacggt cgggtccaga tattcgtatc tgtcgagtag
      241 agtgtgggct cttgccatgt gtatgtgggc caggcatcaa ataaaacgaa aggctcagtc
      301 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      361 cactggctca ccttcgggtg ggcctttctg cgtttata
//
