LOCUS       sequence-247589-       15512 bp    DNA     circular SYN 15-APR-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15512)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX174
FEATURES             Location/Qualifiers
     source          1..15512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5658..5682)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5711..5800
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5834..6086
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6515..6767)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7235..8260)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7235..8260)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8270..8614)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8724..8741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8724..8740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8733..8755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8880..8899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9090..9111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9358..9382
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10978..12126)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12425..13219)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLLLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13411..14178
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14871..15502
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggctgt
     4621 agagttaatg actagtggtt atagcagaag ggatagtttc tcaaccaaaa tggaagaaaa
     4681 cgccgtacaa gaagcagcca ccgatggcct acaaagcgtt gagaaattaa tccgtttgct
     4741 ttctcaatct catcaaaacc aacaacaaca acaacaaaaa ttagatcaaa atccctctgt
     4801 ttctgctgat tacacagccg tagctgatgt tgctgttaac aaattcaaaa agttcatttc
     4861 tttacttgac aaaaacagaa ctggtcatgc cagatttcgt aaaggcccaa tttctactcc
     4921 tcctcctaaa ccccagcaac aaagattaga tcaaaactct atcaaaaatc ataaccttca
     4981 aatagaagaa accgaaaagc cacaaaaaaa ttctcccaaa atctattgtc caacacctat
     5041 gcaacgttta cctccgttac cacataacca tcttcaattg gtcaaaaatg ggtcgattga
     5101 gagaaaagag tcatctacta ctattaattt tgcttctgca tctccggcta attcgtttat
     5161 gtcatcttta acaggagaaa cagagagttt gcaacattct ttgtcttctg gttttcaaat
     5221 aacaaatatt tctactgttt catctgccgg ccggccgccg ctttctactt catttaaaag
     5281 gaagtgtagt tctttggacg atactgccct taagtgtaac agcgctggcg gttcctctgg
     5341 tcgttgccac tgcccaaaga aaaggaaatc aagagtcaaa agagtggtga gagtcccagc
     5401 tataagcatg aaaatggctg atattccacc tgatgattat tcttggagaa aatatggtca
     5461 aaagccaatt aagggatctc ctcatcctag gggatattac aaatgtagta gtgtaagagg
     5521 atgtccagca cgtaaacatg tggaaagagc attagatgat ccaactatgt taattgttac
     5581 atatgaagga gaacacaatc attcccattc aattacagaa tcaccagctg ctcatgtcct
     5641 tgaatcttcc cccggggacc cagctttctt gtacaaagtg gttgataaca gcgggttaat
     5701 taacatcttt tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     5761 ctatgcagga tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag
     5821 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5881 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5941 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     6001 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     6061 cgcggtgtca tctatgttac tagatcggga attagcttca tcaacgcaag acatgcgcac
     6121 gaccgtctga caggagagga atttccgacg agcacagaaa ggacttgctc ttggacgtag
     6181 gcctatttct caggcacatg tatcaagtgt tcggacgtgg gttttcgatg gtgtatcagc
     6241 cgccgccaac tgggagatga ggaggctttc ttggggggca gtcagcagtt catttcacaa
     6301 gacagaggaa cttgtaagga gatgcactga tttatcttgg cgcaaaccag caggacgaat
     6361 tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg gcatgagcag aaacttccaa
     6421 ttcgaaacag tttggagagg ttgtttttgg gcataccttt tgttagtcag cctctcgatt
     6481 gctcatcgtc attacacagt accgaagttt gatcgatcta gtaacataga tgacaccgcg
     6541 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     6601 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     6661 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     6721 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttcgacgcac
     6781 tccttcttta ctccaccatc tcgtccttat tgaaaacgtg ggtagcacca aaacgaatca
     6841 agtcgctgga actgaagtta ccaatcacgc tggatgattt gccagttgga ttaatcttgc
     6901 ctttccccgc atgaataata ttgatgaatg catgcgtgag gggtatttcg attttggcaa
     6961 tagctgcaat tgccgcgaca tcctccaacg agcataattc ttcagaaaaa tagcgatgtt
     7021 ccatgttgtc agggcatgca tgatgcacgt tatgaggtga cggtgctagg cagtattccc
     7081 tcaaagtttc atagtcagta tcatattcat cattgcattc ctgcaagaga gaattgagac
     7141 gcaatccaca cgctgcggca accttccggc gttcgtggtc tatttgctct tggacgttgc
     7201 aaacgtaagt gttggatccc ggtcggcatc tactctattc ctttgccctc ggacgagtgc
     7261 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     7321 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     7381 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     7441 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     7501 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     7561 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     7621 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     7681 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     7741 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     7801 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     7861 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     7921 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     7981 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     8041 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     8101 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     8161 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     8221 acttctcgac agacgtcgcg gtgagttcag gctttttcat atcggggtcg tcctctccaa
     8281 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     8341 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     8401 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     8461 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     8521 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     8581 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     8641 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga cggatctcta
     8701 ggacgcgtcc tagaagctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8761 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8821 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc
     8881 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8941 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9001 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9061 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9121 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc
     9181 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     9241 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     9301 accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     9361 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     9421 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     9481 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     9541 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     9601 agcgttgctg cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc
     9661 ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat
     9721 aggaaatcgc tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa
     9781 cgttgacgat atcaactccc ctatccattg ctcaccgaat ggtacaggtc ggggacccga
     9841 agttccgact gtcggcctga tgcatccccg gctgatcgac cccagatctg gggctgagaa
     9901 agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt
     9961 aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag
    10021 gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg
    10081 ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca
    10141 cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta
    10201 gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca
    10261 ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca
    10321 gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa
    10381 acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc
    10441 agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc
    10501 gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca
    10561 gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga
    10621 tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct
    10681 ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca
    10741 gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct
    10801 gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc
    10861 cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg
    10921 aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta
    10981 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
    11041 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
    11101 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
    11161 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
    11221 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
    11281 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
    11341 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
    11401 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
    11461 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
    11521 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
    11581 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
    11641 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
    11701 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
    11761 caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
    11821 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
    11881 ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
    11941 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac
    12001 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
    12061 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
    12121 attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc
    12181 ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc
    12241 ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac
    12301 gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca
    12361 cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta
    12421 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    12481 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    12541 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    12601 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    12661 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    12721 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    12781 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    12841 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    12901 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    12961 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    13021 gcaatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    13081 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    13141 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    13201 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    13261 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    13321 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    13381 gacggagccg attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt
    13441 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc
    13501 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg
    13561 ctctcactgc cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac
    13621 gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat
    13681 tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta
    13741 acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg
    13801 ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg
    13861 aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg
    13921 agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa
    13981 tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg
    14041 cataacctga atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca
    14101 aaatcggtgg agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa
    14161 taagttggag tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg
    14221 gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata
    14281 agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa
    14341 aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc
    14401 ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa
    14461 ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct
    14521 gggtttatcg cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc
    14581 aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag
    14641 ggcaggccgc cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat
    14701 gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc
    14761 gttactgccc cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg
    14821 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg
    14881 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca
    14941 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta
    15001 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag
    15061 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa
    15121 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact
    15181 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc
    15241 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt
    15301 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt
    15361 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg
    15421 cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag
    15481 ggcaaccagc ccggtgagcg tcggaaaggc gc
//
