LOCUS       sequence-247617-       11881 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Tet2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Wang Y, Zhang Y
  TITLE     Regulation of TET protein stability by calpains.
  JOURNAL   Cell Rep. 2014 Jan 30;6(2):278-84. doi: 
            10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9.
  PUBMED    24412366
REFERENCE   2  (bases 1 to 11881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.12.031"; journalName: "Cell Rep"; date: 
            "2014-01-30- 30"; volume: "6"; issue: "2"; pages: "278-84"
COMMENT     Sequence Label: pcDNA3-Tet2
FEATURES             Location/Qualifiers
     source          1..11881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2552..2755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2800..2819
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2800..2818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2855..3427
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3473..3496
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(9371..9389)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9372..9389)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9409..9426)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9415..9639
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9685..10113
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9772..9791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9982..10003
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10122..10142)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10127..10456
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10307..10442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10369..10388
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10523..11317
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10577..10596)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11187..11206
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11491..11612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11528..11547)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11582..11601
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11661..11677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11661..11677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11674..11696)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11685..11701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11709..11715,11716..11733,11734..11739))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11754..11775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     2761 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
     2821 gacccaagct agagtcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     2881 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     2941 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     3001 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     3061 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     3121 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     3181 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     3241 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     3301 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     3361 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     3421 cctacagctc ctgggcaacg tgctggtttg tgctgtcgac cccaagctta tggactacaa
     3481 agacgatgac gacaagggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttt
     3541 agaacaggac agaaccaccc atgctgaggg caccagactg agtccattcc tgatagcacc
     3601 accttctccc atcagccata cagaacctct ggctgtcaaa ctccagaatg gaagcccgtt
     3661 agcagagaga cctcatccag aagtaaatgg agacaccaag tggcaatctt cccaaagctg
     3721 ttatggaata tcccacatga aaggcagcca gagcagtcat gagagtccac atgaagacag
     3781 agggtattcc aggtgcttac agaatggagg gataaaacgc acagtcagtg aaccatctct
     3841 ctctgggctc catccaaaca agatattgaa actagaccaa aaagctaagg gagaaagcaa
     3901 tatcttcgag gaaagccaag aaagaaacca cggtaaaagc agccgtcagc caaatgtctc
     3961 cggactaagt gataatggag agcccgtgac ctcgaccacc caggaaagtt caggtgcaga
     4021 tgctttccca acacggaact acaatggagt tgaaattcag gttctcaacg agcaggaagg
     4081 ggaaaaaggc aggagcgtta cattacttaa aaacaagatt gtgctaatgc ctaatggtgc
     4141 tacagtttct gcccattctg aggagaacac acgtggtgaa ctcctggaaa aaacacagtg
     4201 ttatccagat tgtgtctcca ttgccgtgca gagtaccgca tctcatgtaa acacccctag
     4261 cagtcaggct gctatcgagt tgtcccacga gatccctcaa ccatcgctta cctcagcgca
     4321 gatcaatttc tcccagacct caagcttgca gctgcctcca gagccagctg caatggtgac
     4381 taaggcctgt gatgctgata atgccagtaa accagctata gtaccaggta cctgtccttt
     4441 tcagaaagca gaacaccaac aaaagtcagc tttggacata ggcccatctc gtgcagaaaa
     4501 caaaaccatc caaggaagca tggagctatt tgctgaagaa tactatccta gttccgaccg
     4561 gaatttgcaa gcttcgcatg gcagctctga acagtattca aagcaaaagg aaaccaatgg
     4621 tgcttacttc aggcaaagct cgaagttccc taaagattcc atctctccca ctactgtgac
     4681 cccaccgtca caatcacttc ttgctccccg tcttgttctt cagcctcctt tagaaggaaa
     4741 aggcgctcta aatgatgtag ctttggaaga acaccatgac taccccaacc gaagcaaccg
     4801 aactctttta agggaaggga aaatagacca tcaacccaag acatcatcta gccagagtct
     4861 gaatccatct gtacatacac ccaacccccc cttgatgctt ccagaacagc atcagaatga
     4921 ttgtggctca ccgagccctg aaaagtcaag aaaaatgtca gaatatctca tgtattacct
     4981 gccaaatcat ggccacagtg gaggtttaca agaacatagc caatacctga tggggcacag
     5041 ggagcaagag attccgaagg atgcaaacgg gaaacaaacg caaggctctg tacaggcagc
     5101 acctggctgg atagaactga aagccccgaa tttgcatgaa gcactccatc agacaaaacg
     5161 caaggatata tccttgcact cagtcctcca ctctcagacc ggccctgtca atcagatgag
     5221 ctccaaacag tccactggca atgtcaacat gccaggagga ttccaaaggc taccttacct
     5281 ccagaaaaca gcccagccag agcagaaggc acaaatgtac caagtgcaag tgaaccaagg
     5341 accgtctcca ggtatggggg accaacatct tcagttccag aaagctttat accaggagtg
     5401 catccccagg acagatccgt catctgaggc tcacccgcaa gcaccgagcg ttcctcagta
     5461 tcatttccag caaagagtaa atccctccag tgataagcat ttgagtcaac aggccacaga
     5521 gactcaacgg ttatcaggct ttttacaaca tactcctcag acgcaggcat cacaaacacc
     5581 agcatcccag aactcaaatt tccctcaaat ctgccagcag cagcagcagc agcagcagca
     5641 gttacagagg aagaataaag agcaaatgcc tcagactttc tctcatctcc aaggtagcaa
     5701 tgataagcaa agagaaggct cgtgctttgg ccagattaaa gtggaagaaa gcttttgtgt
     5761 cggaaatcag tactccaaat caagtaattt ccaaactcac aataataccc aaggggggtt
     5821 ggagcaagta caaaatataa ataaaaattt tccttattcg aagatcttaa caccaaattc
     5881 gagcaactta cagattctcc cttcaaatga cacacacccg gcttgtgagc gggaacaagc
     5941 tctacatccc gtaggaagta agacctcaaa cctgcagaac atgcagtatt tcccgaataa
     6001 tgtgacccca aatcaggacg ttcaccggtg ctttcaggaa caagcgcaga agcctcagca
     6061 agcttcgtct ctacaggggc ttaaggacag aagccagggt gagtctccag ccccaccagc
     6121 tgaggcagct caacagaggt atttggtgca taatgaagca aaggcactcc ctgtgcctga
     6181 gcaaggagga agtcagacac agacccctcc tcagaaggac actcagaagc acgctgcctt
     6241 aaggtggctt ctcttacaga agcaagaaca gcagcaaaca cagcaatccc agcctggtca
     6301 taaccagatg cttaggccaa tcaagactga gcctgtatcc aaaccttctt cctatagata
     6361 ccccttgtca ccgccacaag aaaatatgtc cagcaggata aagcaagaga tctcctctcc
     6421 aagccgtgac aatgggcagc caaagagcat cattgagacc atggaacagc acctgaagca
     6481 gtttcagctc aagtcactct gtgactataa ggctctgact ctcaagtcac agaaacacgt
     6541 gaaagtgcca acagatatcc aggctgcaga atcggagaac cacgcccgag ctgcagagcc
     6601 tcaagcaacc aaaagcacag attgttctgt tctcgacgat gtttcagaat cagatactcc
     6661 tggtgaacaa agtcagaatg gcaaatgtga aggatgcaat ccagacaaag atgaagctcc
     6721 ttattatacc catctaggag ctggtcctga tgtggcagct attagaacac tcatggaaga
     6781 aaggtatgga gagaagggta aagctattag gattgaaaaa gtcatatata ctggtaaaga
     6841 aggcaagagc tctcagggat gtcctattgc taaatgggta tatcggagat cgagtgagga
     6901 ggagaaacta ctgtgtttgg tacgagtgcg acctaaccac acatgtgaga cggcggtgat
     6961 ggtaattgcc atcatgttgt gggacggaat cccaaagcta ctcgcatcag aactctactc
     7021 agaacttaca gatatcttgg gcaagtgtgg catatgcacc aaccgtcgct gttctcagaa
     7081 tgaaacgaag aaaaagcaat caccacccag aaactgttgt tgtcagggtg agaatccaga
     7141 gacctgtggt gcctcctttt cttttggttg ttcttggagc atgtactata atggatgtaa
     7201 gtttgccaga agcaagaaac caaggaaatt taggctacat ggagctgagc caaaagagga
     7261 agagagacta ggttctcatt tgcaaaacct ggctactgtc attgctccaa tatacaagaa
     7321 gcttgcaccc gatgcataca ataatcaggt tgaatttgaa caccaagccc cagactgctg
     7381 tttgggtctg aaggaaggcc ggccattctc aggagtcact gcatgtttgg acttctctgc
     7441 tcattcccac agagaccagc agaacatgcc aaatggcagt acagtggtgg tcaccctcaa
     7501 tagagaagac aatcgagaag tcggagctaa gcctgaggat gagcagttcc acgtgctgcc
     7561 tatgtacatc atcgcccctg aggatgagtt tgggagtacg gaaggccagg agaagaagat
     7621 acggatgggg tccattgagg ttctgcagtc atttcggagg agaagggtca taaggatagg
     7681 agagctgccc aagagttgca agaagaaagc ggagcccaag aaagccaaga ccaagaaagc
     7741 agctcgaaag cattcctctc tggagaactg ctccagtagg actgagaagg gaaagtcttc
     7801 ctcacataca aagctgatgg aaaatgcaag ccatatgaaa caaatgacag cacaaccgca
     7861 gctttcgggc ccggtcatcc ggcagccacc aacactccag aggcaccttc agcaagggca
     7921 gaggccacag cagccgcagc cacctcagcc gcagccgcag acgacacctc agccacagcc
     7981 acagccacag catatcatgc ccggtaactc tcagtctgtt ggttctcatt gttctggatc
     8041 caccagtgtc tacacgagac agcctactcc tcacagtcct tatcccagct cagcacacac
     8101 ctcagatatt tatggagata ccaaccatgt gaacttttac cccacttcat ctcatgcctc
     8161 gggttcatat ttgaatcctt ctaattacat gaacccctac cttgggcttt tgaatcagaa
     8221 taaccaatat gcaccttttc catacaatgg gagtgtgcca gtggacaatg gttccccttt
     8281 cttaggttct tattcccccc aggctcagtc cagggatcta catagatatc caaaccagga
     8341 ccatctcacc aatcagaact taccacccat ccacaccctt caccaacaga cgtttgggga
     8401 cagtccctct aagtacttaa gttatggaaa ccaaaatatg cagagagatg ccttcactac
     8461 taactccacc ctaaaaccaa atgtacacca cctagcaacg ttttctcctt accccacccc
     8521 caagatggat agtcatttca tgggagctgc ctccagatca ccatacagcc acccacacac
     8581 tgactacaaa accagtgagc atcatctacc ctctcacacg gtctacagct acacggcagc
     8641 agcttcgggg agcagttcca gccacgcctt ccacaacaag gagaatgaca acatagccaa
     8701 tgggctctca agagtgcttc cagggtttaa tcatgataga actgcttctg cccaagaact
     8761 attatacagt ctgactggca gcagtcagga gaagcagcct gaggtgtcag gccaggatgc
     8821 agctgctgtg caggaaattg agtattggtc agatagtgag cacaactttc aggatccttg
     8881 cattggaggg gtggctatag ccccaactca tgggtcaatt cttattgagt gtgcaaagtg
     8941 tgaggttcat gccacaacca aagtaaacga tcccgaccgg aatcacccca ccaggatctc
     9001 acttgtactg tataggcata agaatttgtt tctaccaaaa cattgtttgg ctctctggga
     9061 agccaaaatg gctgaaaagg cccggaaaga ggaagagtgc ggaaagaatg gatcagacca
     9121 cgtgtctcag aaaaatcatg gcaaacagga aaagcgtgag cccacagggc cacaggaacc
     9181 cagttacctg cgtttcatcc agtctcttgc tgagaacaca gggtctgtga ctacggattc
     9241 taccgtgact acatcaccat atgctttcac tcaggtcaca gggccttaca acacatttgt
     9301 atgaaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag
     9361 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     9421 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9481 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9541 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9601 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9661 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9841 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9901 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9961 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    10021 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    10081 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
    10141 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10201 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10261 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    10321 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10381 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10441 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
    10501 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
    10561 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    10621 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    10681 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
    10741 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    10801 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    10861 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    10921 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    10981 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    11041 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    11101 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    11161 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    11221 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    11281 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    11341 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    11401 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    11461 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    11521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    11641 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    11701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    11761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    11881 t
//
