LOCUS       sequence-247695-        2345 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PrpoB
FEATURES             Location/Qualifiers
     source          1..2345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(551..568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(661..1249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(741..760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1423..2217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2218..2322)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcaggag ggttggaatg tgtattatca taataattac
      421 ttgagaccgg taccctctgg gcctcatggg ccttcctttc actgcccgct ttccagtcgg
      481 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
      541 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
      601 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      661 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      721 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      781 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      841 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      901 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      961 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1021 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1081 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1141 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1201 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1261 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1321 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1381 atcaatctaa agtatatatg agtaaacttg gtctgacagt tattagaaaa attcatccag
     1441 cagacgataa aacgcaatac gctggctatc cggtgccgca atgccataca gcaccagaaa
     1501 acgatccgcc cattcgccgc ccagttcttc cgcaatatca cgggtggcca gcgcaatatc
     1561 ctgataacga tccgccacgc ccagacggcc gcaatcaata aagccgctaa aacggccatt
     1621 ttccaccata atgttcggca ggcacgcatc accatgggtc accaccagat cttcgccatc
     1681 cggcatgctc gctttcagac gcgcaaacag ctctgccggt gccaggccct gatgttcttc
     1741 atccagatca tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg
     1801 atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc agggtatgca gacgacgcat
     1861 ggcatccgcc ataatgctca ctttttctgc cggcgccaga tggctagaca gcagatcctg
     1921 acccggcact tcgcccagca gcagccaatc acggcccgct tcggtcacca catccagcac
     1981 cgccgcacac ggaacaccgg tggtggccag ccagctcaga cgcgccgctt catcctgcag
     2041 ctcgttcagc gcaccgctca gatcggtttt cacaaacagc accggacgac cctgcgcgct
     2101 cagacgaaac accgccgcat cagagcagcc aatggtctgc tgcgcccaat catagccaaa
     2161 cagacgttcc acccacgctg ccgggctacc cgcatgcagg ccatcctgtt caatcatact
     2221 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2281 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2341 gccac
//
