LOCUS       sequence-247899-        2365 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-psbN 5'UTR
FEATURES             Location/Qualifiers
     source          1..2365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(571..588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(681..1269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(761..780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1443..2237)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2238..2342)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact ctatcataga tccattgggg tcttgaaatt
      421 ttataaatat gataagaaat gtgagaccgg taccctctgg gcctcatggg ccttcctttc
      481 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaacatg gtcatagctg
      541 tttccttgcg tattgggcgc tctccgcttc ctcgctcact gactcgctgc gctcggtcgt
      601 tcgggtaaag cctggggtgc ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa
      661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1081 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1441 tattagaaaa attcatccag cagacgataa aacgcaatac gctggctatc cggtgccgca
     1501 atgccataca gcaccagaaa acgatccgcc cattcgccgc ccagttcttc cgcaatatca
     1561 cgggtggcca gcgcaatatc ctgataacga tccgccacgc ccagacggcc gcaatcaata
     1621 aagccgctaa aacggccatt ttccaccata atgttcggca ggcacgcatc accatgggtc
     1681 accaccagat cttcgccatc cggcatgctc gctttcagac gcgcaaacag ctctgccggt
     1741 gccaggccct gatgttcttc atccagatca tcctgatcca ccaggcccgc ttccatacgg
     1801 gtacgcgcac gttcaatacg atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc
     1861 agggtatgca gacgacgcat ggcatccgcc ataatgctca ctttttctgc cggcgccaga
     1921 tggctagaca gcagatcctg acccggcact tcgcccagca gcagccaatc acggcccgct
     1981 tcggtcacca catccagcac cgccgcacac ggaacaccgg tggtggccag ccagctcaga
     2041 cgcgccgctt catcctgcag ctcgttcagc gcaccgctca gatcggtttt cacaaacagc
     2101 accggacgac cctgcgcgct cagacgaaac accgccgcat cagagcagcc aatggtctgc
     2161 tgcgcccaat catagccaaa cagacgttcc acccacgctg ccgggctacc cgcatgcagg
     2221 ccatcctgtt caatcatact cttccttttt caatattatt gaagcattta tcagggttat
     2281 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2341 cgcacatttc cccgaaaagt gccac
//
