LOCUS       sequence-248071-        3371 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 
            (pBBR322/ROP; medium copy number)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 91 to 125)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP29-1A(lacZ)
FEATURES             Location/Qualifiers
     source          1..3371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        91..125
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    486..506
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      540..634
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(755..774)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             766..1581
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1748..1856
                     /note="incP origin of transfer"
     rep_origin      1892..2480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2381..2400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2634..2651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2666..2806
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2732..2751)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2892..2914
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2908..3099)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3279..3365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagccggtg acccgaaact
      181 gctgctggaa gttccgcacg ctatctcttc ttctccgggt aactctctgg ctgttgttct
      241 gcaacgtcgt gactgggaaa acccgggtgt tacccagctg aaccgtctgg ctgctcaccc
      301 gccgttcgct tcttggcgta actctgaaga agctcgtacc gaccgtccgt cccagcagct
      361 gcgttctctg atccgtctgc tgaccaaacc ggaacgtaaa ctgtcttggc tgctgccgcc
      421 gctgtctaac aacggttcgt aacttctgac tgagttgcac gctggtctct cgctaatgtg
      481 agacgtacta gtagcggccg ctgcagggag ttgtcttcga agacttcgct ctagtcttgg
      541 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
      601 cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt
      661 aaatttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      721 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcag
      781 cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca tggatgcgga tctgtatggc
      841 tataaatggg cgcgtgataa cgtgggtcag agcggcgcga ccatttatcg tctgtatggc
      901 aaaccggatg cgccggaact gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg
      961 accgatgaaa tggtgcgtct gaactggctg accgaattta tgccgctgcc gaccattaaa
     1021 cattttattc gcaccccgga tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc
     1081 gcgtttcagg tgctggaaga atatccggat agcggcgaaa acattgtgga tgcgctggcc
     1141 gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt
     1201 gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg gcctggtgga tgcgagcgat
     1261 tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt ggaaagaaat gcataaactg
     1321 ctgccgttta gcccggatag cgtggtgacc cacggcgatt ttagcctgga taacctgatt
     1381 ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt
     1441 tatcaggatc tggccattct gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa
     1501 cgtctgtttc agaaatatgg cattgataat ccggatatga acaaactgca atttcatctg
     1561 atgctggatg aatttttcta ataattaatt ggaccgcggt ccgcgcgttg tccttttccg
     1621 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1681 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1741 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1801 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1861 ccggccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2641 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     2701 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2761 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     2821 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2881 tgtgacagtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2941 gaggcagctg cggtaaagct catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg
     3001 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     3061 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     3121 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     3181 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     3241 gatgcggcgg gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     3301 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     3361 atttgatgcc t
//
