LOCUS       sequence-24849-e         819 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p47 PX(1-121)/p40phox(136-339)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgnganattc cntctnaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggctagcat
      121 gactggtgga cagcaaatgg gtcgcggatc cgaattcatg ggggacacct tcatccgtca
      181 catcgccctg ctgggctttg agaagcgctt cgtacccagc cagcactatg tgtacatgtt
      241 cctggtgaaa tggcaggacc tgtcggagaa ggtggtctac cggcgcttca ccgagatcta
      301 cgagttccat aaaaccttaa aagaaatgtt ccctattgag gcaggggcga tcaatccaga
      361 gaacaggatc atcccccacc tcccagctcc caagtggttt gacgggcagc gggccgccga
      421 gaaccgccag ggcacactta ccgagtactg cagcacgctc atgagcctgc ccaccaagat
      481 ctcccgctgt ccccacctcc tcgacttctt caaggtgcgc cctgatgacc tccagtcgcc
      541 ctatgactca gagcaggtgc cccaggcact ccgccggctc cgcccgcgca cccggaaagt
      601 caagagcgtg tccccacagg gcaacagcgt tgaccgcatg gntcacacca aaagaaaagn
      661 ctctattgat tccnggantg gaaacagcaa ctgtggagct gaatttcaaa gctggagatg
      721 tgatcttntc ctcctcagtc gnatcaacaa agactggctg ganggcactg tcngggancc
      781 acgggcatnt tcctctctcn tncggaaanc ctnaaaant
//
