LOCUS       sequence-248595-        1905 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes ConL4 and picornavius peptide 2A (P2A) element as a Type 1 
            part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_033
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             691..747
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      complement(767..801)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(872..1531)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1446..1465
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1713..1905,1..396)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
       61 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      481 tctgtggata accgtagtcg gtctcaccct gaattcgcat ctagagcctg cactagacga
      541 actaggcaag atgcgtccaa tccgtctaaa catggtgaca acgctggaca gatgacgtaa
      601 caccgagcca catcctgaaa tcgaggcagg ctaaccgaaa ccgtgacaat gcaaagagac
      661 agcctgaccg tctcaggtag ggggagcggc gcgacgaact tttcgttgct aaagcaagcg
      721 ggggacgtgg aagaaaaccc ggggcctgga acgtgagacc agaccaataa aaaacgcccg
      781 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
      841 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
      901 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
      961 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1021 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1081 cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1141 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1201 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1261 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1321 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1381 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1441 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1501 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1561 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1621 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1681 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1741 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1801 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1861 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgta
//
