LOCUS       sequence-248782-       13375 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression of ALK F1174L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ALK-F1174L
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8312..8336)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8389..8430
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8451..9018
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8468..9020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8612..8629)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8839..8858
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9032..9034,9035..9037,9038..9751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9032..9034,9035..9037,9038..9748)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9077..9098)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9338..9357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9685..9706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9765..10353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9818..9838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10236..10247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10428..10661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10780..10798)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10866..10970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10971..11039,11040..11831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11189..11208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12002..12590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12491..12510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12744..12761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13001..13022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13037..13042,13043..13060,13061..13067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13075..13091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13080..13102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13099..13115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13099..13115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13165..13184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg ggagccatcg ggctcctgtg gctcctgccg
     3481 ctgctgcttt ccacggcagc tgtgggctcc gggatgggga ccggccagcg cgcgggctcc
     3541 ccagctgcgg ggccgccgct gcagccccgg gagccactca gctactcgcg cctgcagagg
     3601 aagagtctgg cagttgactt cgtggtgccc tcgctcttcc gtgtctacgc ccgggaccta
     3661 ctgctgccac catcctcctc ggagctgaag gctggcaggc ccgaggcccg cggctcgcta
     3721 gctctggact gcgccccgct gctcaggttg ctggggccgg cgccgggggt ctcctggacc
     3781 gccggttcac cagccccggc agaggcccgg acgctgtcca gggtgctgaa gggcggctcc
     3841 gtgcgcaagc tccggcgtgc caagcagttg gtgctggagc tgggcgagga ggcgatcttg
     3901 gagggttgcg tcgggccccc cggggaggcg gctgtggggc tgctccagtt caatctcagc
     3961 gagctgttca gttggtggat tcgccaaggc gaagggcgac tgaggatccg cctgatgccc
     4021 gagaagaagg cgtcggaagt gggcagagag ggaaggctgt ccgcggcaat tcgcgcctcc
     4081 cagccccgcc ttctcttcca gatcttcggg actggtcata gctccttgga atcaccaaca
     4141 aacatgcctt ctccttctcc tgattatttt acatggaatc tcacctggat aatgaaagac
     4201 tccttccctt tcctgtctca tcgcagccga tatggtctgg agtgcagctt tgacttcccc
     4261 tgtgagctgg agtattcccc tccactgcat gacctcagga accagagctg gtcctggcgc
     4321 cgcatcccct ccgaggaggc ctcccagatg gacttgctgg atgggcctgg ggcagagcgt
     4381 tctaaggaga tgcccagagg ctcctttctc cttctcaaca cctcagctga ctccaagcac
     4441 accatcctga gtccgtggat gaggagcagc agtgagcact gcacactggc cgtctcggtg
     4501 cacaggcacc tgcagccctc tggaaggtac attgcccagc tgctgcccca caacgaggct
     4561 gcaagagaga tcctcctgat gcccactcca gggaagcatg gttggacagt gctccaggga
     4621 agaatcgggc gtccagacaa cccatttcga gtggccctgg aatacatctc cagtggaaac
     4681 cgcagcttgt ctgcagtgga cttctttgcc ctgaagaact gcagtgaagg aacatcccca
     4741 ggctccaaga tggccctgca gagctccttc acttgttgga atgggacagt cctccagctt
     4801 gggcaggcct gtgacttcca ccaggactgt gcccagggag aagatgagag ccagatgtgc
     4861 cggaaactgc ctgtgggttt ttactgcaac tttgaagatg gcttctgtgg ctggacccaa
     4921 ggcacactgt caccccacac tcctcaatgg caggtcagga ccctaaagga tgcccggttc
     4981 caggaccacc aagaccatgc tctattgctc agtaccactg atgtccccgc ttctgaaagt
     5041 gctacagtga ccagtgctac gtttcctgca ccgatcaaga gctctccatg tgagctccga
     5101 atgtcctggc tcattcgtgg agtcttgagg ggaaacgtgt ccttggtgct agtggagaac
     5161 aaaaccggga aggagcaagg caggatggtc tggcatgtcg ccgcctatga aggcttgagc
     5221 ctgtggcagt ggatggtgtt gcctctcctc gatgtgtctg acaggttctg gctgcagatg
     5281 gtcgcatggt ggggacaagg atccagagcc atcgtggctt ttgacaatat ctccatcagc
     5341 ctggactgct acctcaccat tagcggagag gacaagatcc tgcagaatac agcacccaaa
     5401 tcaagaaacc tgtttgagag aaacccaaac aaggagctga aacccgggga aaattcacca
     5461 agacagaccc ccatctttga ccctacagtt cattggctgt tcaccacatg tggggccagc
     5521 gggccccatg gccccaccca ggcacagtgc aacaacgcct accagaactc caacctgagc
     5581 gtggaggtgg ggagcgaggg ccccctgaaa ggcatccaga tctggaaggt gccagccacc
     5641 gacacctaca gcatctcggg ctacggagct gctggcggga aaggcgggaa gaacaccatg
     5701 atgcggtccc acggcgtgtc tgtgctgggc atcttcaacc tggagaagga tgacatgctg
     5761 tacatcctgg ttgggcagca gggagaggac gcctgcccca gtacaaacca gttaatccag
     5821 aaagtctgca ttggagagaa caatgtgata gaagaagaaa tccgtgtgaa cagaagcgtg
     5881 catgagtggg caggaggcgg aggaggaggg ggtggagcca cctacgtatt taagatgaag
     5941 gatggagtgc cggtgcccct gatcattgca gccggaggtg gtggcagggc ctacggggcc
     6001 aagacagaca cgttccaccc agagagactg gagaataact cctcggttct agggctaaac
     6061 ggcaattccg gagccgcagg tggtggaggt ggctggaatg ataacacttc cttgctctgg
     6121 gccggaaaat ctttgcagga gggtgccacc ggaggacatt cctgccccca ggccatgaag
     6181 aagtgggggt gggagacaag agggggtttc ggagggggtg gaggggggtg ctcctcaggt
     6241 ggaggaggcg gaggatatat aggcggcaat gcagcctcaa acaatgaccc cgaaatggat
     6301 ggggaagatg gggtttcctt catcagtcca ctgggcatcc tgtacacccc agctttaaaa
     6361 gtgatggaag gccacgggga agtgaatatt aagcattatc taaactgcag tcactgtgag
     6421 gtagacgaat gtcacatgga ccctgaaagc cacaaggtca tctgcttctg tgaccacggg
     6481 acggtgctgg ctgaggatgg cgtctcctgc attgtgtcac ccaccccgga gccacacctg
     6541 ccactctcgc tgatcctctc tgtggtgacc tctgccctcg tggccgccct ggtcctggct
     6601 ttctccggca tcatgattgt gtaccgccgg aagcaccagg agctgcaagc catgcagatg
     6661 gagctgcaga gccctgagta caagctgagc aagctccgca cctcgaccat catgaccgac
     6721 tacaacccca actactgctt tgctggcaag acctcctcca tcagtgacct gaaggaggtg
     6781 ccgcggaaaa acatcaccct cattcggggt ctgggccatg gcgcctttgg ggaggtgtat
     6841 gaaggccagg tgtccggaat gcccaacgac ccaagccccc tgcaagtggc tgtgaagacg
     6901 ctgcctgaag tgtgctctga acaggacgaa ctggatttcc tcatggaagc cctgatcatc
     6961 agcaaactca accaccagaa cattgttcgc tgcattgggg tgagcctgca atccctgccc
     7021 cggttcatcc tgctggagct catggcgggg ggagacctca agtccttcct ccgagagacc
     7081 cgccctcgcc cgagccagcc ctcctccctg gccatgctgg accttctgca cgtggctcgg
     7141 gacattgcct gtggctgtca gtatttggag gaaaaccact tcatccaccg agacattgct
     7201 gccagaaact gcctcttgac ctgtccaggc cctggaagag tggccaagat tggagacttc
     7261 gggatggccc gagacatcta cagggcgagc tactatagaa agggaggctg tgccatgctg
     7321 ccagttaagt ggatgccccc agaggccttc atggaaggaa tattcacttc taaaacagac
     7381 acatggtcct ttggagtgct gctatgggaa atcttttctc ttggatatat gccatacccc
     7441 agcaaaagca accaggaagt tctggagttt gtcaccagtg gaggccggat ggacccaccc
     7501 aagaactgcc ctgggcctgt ataccggata atgactcagt gctggcaaca tcagcctgaa
     7561 gacaggccca actttgccat cattttggag aggattgaat actgcaccca ggacccggat
     7621 gtaatcaaca ccgctttgcc gatagaatat ggtccacttg tggaagagga agagaaagtg
     7681 cctgtgaggc ccaaggaccc tgagggggtt cctcctctcc tggtctctca acaggcaaaa
     7741 cgggaggagg agcgcagccc agctgcccca ccacctctgc ctaccacctc ctctggcaag
     7801 gctgcaaaga aacccacagc tgcagagatc tctgttcgag tccctagagg gccggccgtg
     7861 gaagggggac acgtgaatat ggcattctct cagtccaacc ctccttcgga gttgcacaag
     7921 gtccacggat ccagaaacaa gcccaccagc ttgtggaacc caacgtacgg ctcctggttt
     7981 acagagaaac ccaccaaaaa gaataatcct atagcaaaga aggagccaca cgacaggggt
     8041 aacctggggc tggagggaag ctgtactgtc ccacctaacg ttgcaactgg gagacttccg
     8101 ggggcctcac tgctcctaga gccctcttcg ctgactgcca atatgaagga ggtacctctg
     8161 ttcaggctac gtcacttccc ttgtgggaat gtcaattacg gctaccagca acagggcttg
     8221 cccttagaag ccgctactgc ccctggagct ggtcattacg aggataccat tctgaaaagc
     8281 aagaatagca tgaaccagcc tgggccctag aacccagctt tcttgtacaa agtggttgat
     8341 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
     8401 cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga tccctccccc
     8461 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     8521 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     8581 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     8641 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     8701 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     8761 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     8821 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     8881 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     8941 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     9001 tgaaaaacac gatgataata tggccacaca tatggtgagc aagggcgagg agctgttcac
     9061 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     9121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     9181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     9241 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     9301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     9361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     9421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     9481 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     9541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     9601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     9661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     9721 cactctcggc atggacgagc tgtacaagta aatcgataga tcctaatcaa cctctggatt
     9781 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9841 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9901 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9961 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
    10021 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    10081 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    10141 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
    10201 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    10261 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    10321 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta
    10381 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    10441 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
    10501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10621 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
    10681 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt
    10741 aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
    10801 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    10861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    11041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    11101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    11161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
    11221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    12001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    12061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    12121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    12181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
    12841 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    12901 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
    12961 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    13021 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    13081 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
    13141 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    13201 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    13261 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    13321 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
