LOCUS       sequence-249086-        2790 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes human beta actin as a Type 3b part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_001
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1924..1958)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2029..2688)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2603..2622
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctgatgatga tatcgccgcg ctcgtcgttg acaacggctc cggcatgtgc aaggccggct
      841 tcgcgggcga cgatgccccc cgggccgtct tcccctccat cgtggggcgc cccaggcacc
      901 agggcgtgat ggtgggcatg ggtcagaagg attcctatgt gggcgacgag gcccagagca
      961 agagaggcat cctcaccctg aagtacccca tcgagcacgg catcgtcacc aactgggacg
     1021 acatggagaa aatctggcac cacaccttct acaatgagct gcgtgtggct cccgaggagc
     1081 accccgtgct gctgaccgag gcccccctga accccaaggc caaccgcgag aagatgaccc
     1141 agatcatgtt tgaaaccttc aacaccccag ccatgtacgt tgctatccag gctgtgctat
     1201 ccctgtacgc ctctggccgt accactggca tcgtgatgga ctccggtgac ggggtcaccc
     1261 acactgtgcc catctacgag gggtatgccc tcccccatgc catcctgcgt ctggacctgg
     1321 ctggccggga cctgactgac tacctcatga agatcctcac cgagcgcggc tacagcttca
     1381 ccaccacggc cgagcgggaa atcgtgcgtg acattaagga gaagctgtgc tacgtcgccc
     1441 tggacttcga gcaagagatg gccacggctg cttccagctc ctccctggag aagagttacg
     1501 agctgcctga cggccaggtc atcaccattg gcaatgagcg gttccgctgc cctgaggcac
     1561 tcttccagcc ttccttcctg ggcatggagt cctgtggcat ccacgaaact accttcaact
     1621 ccatcatgaa gtgtgacgtg gacatccgca aagacctgta cgccaacaca gtgctgtctg
     1681 gcggcaccac catgtaccct ggcattgccg acaggatgca gaaggagatc actgccctgg
     1741 cacccagcac aatgaagatc aagatcattg ctcctcctga gcgcaagtac tccgtgtgga
     1801 tcggcggctc catcctggcc tcgctgtcca ccttccagca gatgtggatc agcaagcagg
     1861 agtatgacga gtccggcccc tccatcgtcc accgcaaatg cttcggatcc tgagaccaga
     1921 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1981 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     2041 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2101 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2161 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2221 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
     2281 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2341 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     2401 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2461 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2521 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2581 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2641 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     2701 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2761 gtgaaagttg gaacctctta cgtgcccgat
//
