LOCUS       sequence-249100-        8029 bp    DNA     circular SYN 15-APR-2025
DEFINITION  piggyBac-based Dox-inducible expression of Esrrb-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA2944_pPB-TREtight-Esrrb-FLAG-InO
FEATURES             Location/Qualifiers
     source          1..8029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..171
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     163..180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     164..180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   224..258
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        608..922
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    616..634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    652..670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    687..705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    723..741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    759..777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    794..812
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    830..848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     895..919
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          966..1538
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             2919..2984
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(3028..3602,3603..3605,3606..3614)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3195..3212)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3422..3441
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3615..3992
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     regulatory      4005..4014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4011..4664
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(4701..4718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4707..4931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4978..4994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5002..5018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5026..5032,5033..5050,5051..5056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5071..5092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   5319..5381
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5405..5423)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5444..5460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5468..5484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5492..5498,5499..5516,5517..5522))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5537..5558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5675..5692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5846..6434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5926..5945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6605..7396,7397..7465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7228..7247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7466..7570)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(7596..8029,1..22))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7733..7754)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7945..7964
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
       61 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      121 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
      181 gaattgtaat acgactcact atagggcgaa ttgggtacct taaccctaga aagatagtct
      241 gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat agcgcgaatc
      301 cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag gcgtgcttgt
      361 caatgaggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa aatgacgcat
      421 gattatcttt tacgtgactt ttaagattta actcatacga taattatatt gttatttcat
      481 gttctactta cgtgataact tattatatat atattttctt gttatagata tcataattta
      541 aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct atagatctgg
      601 atccgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      661 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      721 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      781 tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      841 gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg
      901 tttagtgaac cgtcagatcg cctggagaat tcgagctcgg tacccgggat cctgagaact
      961 tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt
     1021 tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc
     1081 accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt
     1141 ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt
     1201 aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac
     1261 ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa
     1321 ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa
     1381 atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt
     1441 tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct
     1501 ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg
     1561 ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagac caccatgtcg tccgaagaca
     1621 ggcacctggg ctctagttgc ggctccttca tcaagacgga gccatccagc ccgtcctcgg
     1681 gcattgatgc cctcagccac cacagcccca gcggctcgtc ggacgccagt ggtggctttg
     1741 gcattgccct gagcacccac gccaacggtc tggactcgcc gcctatgttc gcaggtgcgg
     1801 ggctgggagg caacccgtgc cgcaagagct acgaggactg tactagtggt atcatggagg
     1861 actccgccat caaatgcgag tacatgctta acgccatccc caagcgcctg tgcctcgtgt
     1921 gcggggacat tgcctctggc taccactacg gagtggcctc ctgcgaggct tgcaaggcgt
     1981 tcttcaagag aaccattcaa ggcaacatcg agtacaactg cccggccacc aatgaatgtg
     2041 agatcaccaa acggaggcgc aagtcctgtc aggcctgccg attcatgaaa tgcctcaaag
     2101 tggggatgct gaaggaaggt gtgcgccttg accgagttcg aggaggccgc cagaagtaca
     2161 agcgacggct ggattcggag aacagcccct acctgaacct gccgatttcc ccacctgcta
     2221 aaaagccatt gactaagatc gtctcgaatc tactaggggt tgagcaggac aagctgtatg
     2281 ctatgcctcc caacgatatc cccgagggag atatcaaggc cctgaccact ctctgtgaat
     2341 tggcagatcg ggagcttgtg ttcctcatca actgggccaa gcacatccca ggcttcccca
     2401 gtctgacact tggggaccag atgagcctgc tgcagagtgc ctggatggag attctcatct
     2461 tgggcatcgt gtaccgctcg ctcccatacg atgacaagct ggcatacgcc gaggactata
     2521 tcatggatga ggaacactct cgcctggtag ggctgctgga cctttaccga gccatcctgc
     2581 agctggtgcg caggtacaag aaactcaagg tagagaagga agagtttatg atcctcaagg
     2641 ccctggccct cgccaactca gattcgatgt acattgagaa cctggaggcg gtgcagaagc
     2701 tccaggacct gctgcacgag gcgctgcagg actatgagct gagtcagcgc cacgaggagc
     2761 cgcggagggc cggcaagctg ctgctgacgc tgcccctgct gaggcagaca gccgccaaag
     2821 ccgtgcaaca cttctacagt gtgaaactgc agggcaaggt gcccatgcac aaactcttcc
     2881 tggagatgct ggaggccaag gtgtctagtg gcagcggcga ctacaaggac cacgacggcg
     2941 actacaagga ccacgacatc gactacaagg acgacgacga caagtaggcg gccgctcgat
     3001 aagcttgata tcgaattccg cccccccccc ctctccctcc ccccccccta acgttactgg
     3061 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     3121 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     3181 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     3241 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     3301 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     3361 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     3421 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     3481 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     3541 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     3601 atatggccac aaccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
     3661 agaaggcggt gactaaggcg cagaagaaag acggcaagaa gcgcaagcgc agccgcaagg
     3721 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
     3781 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
     3841 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
     3901 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
     3961 ctaaggccgt caccaagtac accagcgcta agctcgagac tagtgccacc atggtgagtg
     4021 tgattaaacc agagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt
     4081 tcacaattga aggtgaaggc acaggcagac cttacgaggg acatcaagag atgacactac
     4141 gcgtcacaat ggccgagggc gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt
     4201 tctgttacgg ccacagagta tttactaaat atccagaaga gataccagac tatttcaaac
     4261 aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg
     4321 cttcagtcag tgcgcatata agccttagag gaaacacctt ctaccacaaa tccaaattta
     4381 ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc
     4441 catcaaccga gaaaattact gccagcgacg gagttctgaa gggtgatgtt acgatgtacc
     4501 taaaacttga aggaggcggc aatcacaaat gccaaatgaa gactacttac aaggcggcaa
     4561 aagagattct tgaaatgcca ggagaccatt acatcggcca tcgcctcgtc aggaaaaccg
     4621 aaggcaacat tactgagcag gtagaagatg cagtagctca ttccgctagc tgaatcgatg
     4681 atctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4741 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4801 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4861 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4921 gggctctatg gcttctgagg cggaaagaac ctgcagccca agcttggcgt aatcatggtc
     4981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc tttgttactt
     5161 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     5221 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     5281 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     5341 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaagagc tccagctttt
     5401 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     5461 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5521 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5581 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5641 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5701 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5761 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5821 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5881 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5941 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6001 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6061 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6601 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6661 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6721 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6781 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6841 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6901 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6961 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7021 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7081 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7141 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7201 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7261 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7321 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7381 ccagcgtttc tgggtgagca aaaacgggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7441 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7501 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7561 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     7621 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7681 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7741 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7801 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7861 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7921 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7981 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctt
//
