LOCUS       sequence-249566-        5153 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-007   EB3-SuperNova//BsaI
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2538..2659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2575..2594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2629..2648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3460..3489
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3511..4098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3999..4018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4110..4137)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4161..4952,4953..5021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4784..4803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5022..5124)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5124..5153,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgtgaa cgtgtactca accagcgtaa caagcgagaa tctgtcaagg cacgacatgt
      901 tggcttgggt gaacgattcc cttcatctga actacaccaa aatcgaacag ctgtgctctg
      961 gagctgccta ttgccagttt atggacatgc tctttcccgg atgtgtgcac cttcggaaag
     1021 tcaaattcca ggcaaaactg gaacatgagt atatccacaa ctttaaggtt ctgcaagcag
     1081 cttttaagaa gatgggcgtg gacaagatca tcccggtgga aaagctcgtg aaagggaagt
     1141 ttcaggacaa tttcgagttc attcagtggt tcaagaagtt ctttgacgcc aactatgatg
     1201 ggaaagatta caatccactg cttgccagac agggacagga tgtcgcaccg ccacccaatc
     1261 ctggcgacca gatcttcaac aagagcaaga aactgattgg gacagcggtt ccacaacgta
     1321 cttcccctac tggccccaag aacatgcaga cgtctggcag actgagcaat gttgctccac
     1381 cctgtatact ccgcaagaat ccacctagtg ctcgaaatgg cggtcatgag acagatgccc
     1441 agatactgga gctcaaccaa cagctggtag acctgaaact gaccgtcgac ggattggaga
     1501 aagagaggga cttctacttc tccaagttgc gggatataga gctcatttgc caggaacacg
     1561 agagcgaaaa ctctcccgtc atcagtggta tcatcggtat tctgtatgcc actgaagaag
     1621 ggtttgcacc tcctgaagat gacgaaattg aggagcacca acaggaggat caagacgagt
     1681 acctggatgg cgggtcaggc ggcagcgagg taggtccagc tctgtttcag tccgatatga
     1741 cgtttaagat cttcatcgat ggcgaagtga acggtcagaa gttcaccata gttgctgatg
     1801 gctcttccaa gttcccacat ggcgacttca atgcccatgc agtgtgtgaa actgggaaac
     1861 tgcctatgag ctggaaaccc atttgccatc tgattcaatg gggagaaccg ttcttcgcga
     1921 ggtatcctga tggcattagc cactttgccc aggagtgctt tccagagggg ctgagtatcg
     1981 accgtactgt gcgcttcgag aatgacggga caatgacctc tcaccacacc tacgagttgg
     2041 acgacacatg tgtggtcagc aggatcacgg tgaactgtga cggctttcag ccagacggac
     2101 ctatcatgcg cgatcagctc gttgacatcc ttccctcaga aacacacatg tttccgcatg
     2161 gacctaatgc cgtcagacag acagcaacta ttgggtttac cactgcagat ggcgggaaga
     2221 tgatgggaca cttcgacagc aagatgacct tcaacggcag tagagccata gagattcctg
     2281 gaccccattt cgtgaccatc atcaccaaac agacacggga cacatccgac aaacgagatc
     2341 acgtctgcca aagggaagtc gcttacgctc actctgtacc ccggattact tcagccatag
     2401 gtagcgatga ggattcaggc tctgattaag gcgccaggta catctccaat acggcgtctt
     2461 gcatagagaa cacgattagc acaccggtta cccacgtgat gcccgaacgg acccatgtca
     2521 tgccagagcg cactggtaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2641 aactcatcaa tgtatcttat catgtctgtc gtcgaattcg agttggcgcg cctgtcattc
     2701 taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca
     2761 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
     2821 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
     2881 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
     2941 ggagggggct gtccccgtga gctcccaggc gcgcctgtca ttctaaatct ctctttcagc
     3001 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     3061 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     3121 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     3181 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccccg
     3241 tgagctccca ggcgcgccaa ctcggtacct cacccgattc tcatcatgga gacacatcag
     3301 gacatctcca ataccgccat ggaaacgcac caagatgcga ctgctcttct gccagtgacg
     3361 gcattcgtct gcaagattaa cggcatacag aaagaaacga tgagacccat tcaggacgag
     3421 cctcagactc cagcgtaact ggactgcaat caactcactg gctcaccttc acgggtgggc
     3481 ctttcttcgg tagaaaatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3541 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3601 accaactctt tttccgaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3661 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3721 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3781 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3841 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3901 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3961 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4021 agtcctgtcg ggtttcgcca cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg
     4081 gggcggagcc tatggaaaaa cgccagcaac gcagaaaggc ccacccgaag gtgagccagg
     4141 tgattacatt tgggccctca ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatgcgg
     4261 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     4321 ccagatttat cagcaataaa ccagccagcc gggagggccg agcgcagaag tgatcctgca
     4381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgc cagaagtaag
     4621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4681 ccatccgtga gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgtaa actctcaagg
     4861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4981 aaaaagggaa taagggcgac acggaaatgt tgaatactca ttttagcttc cttagctcct
     5041 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     5101 ttggaacctc ttacgtgccg atcagtcaaa agcctccggt cggaggcttt tga
//
