LOCUS       sequence-249604-        1795 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1795)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-EMMI-024   Connector(D-G)//Esp3I
FEATURES             Location/Qualifiers
     source          1..1795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..825)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     733..752
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(826..928)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(929..960)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      1107..1136
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      1158..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1757..1784)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tttgggccct cagaaaaact catcgagcat caagtgaaac tgcaatttat tcatatcagg
       61 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
      121 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
      181 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
      241 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
      301 aacaggccag ccattacgct cgtcatcaaa atcactcgca ccaaccaaac cgttattcat
      361 tcgtgattgc gcctgagcga ggcgaaatac gcgatcgccg ttaaaaggac aattacaaac
      421 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
      481 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
      541 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
      601 cagccagttt agcctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
      661 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
      721 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
      781 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcattttag cttccttagc
      841 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
      901 aaagttggaa cctcttacgt gccgatcaag tcaaaagcct ccggtcggag gcttttgact
      961 ttctgctatg gaggtcaggt atgatttaaa cgtctctgga cttagccttt agtgaaccgt
     1021 cagaattaat tcagatcgat ctacagcaga gacgcattca ggacgagcct cagactccag
     1081 cgtaactgga ctgcaatcaa ctcactggct caccttcacg ggtgggcctt tcttcggtag
     1141 aaaatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1261 ccgaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag catcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgca gaaaggccca cccgaaggtg agccaggtga ttaca
//
