LOCUS       sequence-25028-e         807 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Aug 1. ():.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2008 Aug 1. ():."
COMMENT     Sequence Label: PL-452 N-CyPet
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(81..794)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /translation="MSKGEELXGGXVPILVELEGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gcctccccta cccggtagaa tttcgacgac ctgcagccaa gctagcataa cttcgtatag
       61 catacattat acgaagttat tttgtacaat tcatccatac catgggtaat accagcagca
      121 gtaacaaatt cgagcaagac catgtggtct ctcttttcgt ttggatcttt agataaggca
      181 gattgagtgg ataagtaatg gttgtctggc aagatgactg gaccatcacc aattggagta
      241 ttttgttgat aatggtcagc taattgaaca gaaccatcgg taatgttgtg tctggctttg
      301 aagttagctt tgataccatt cttttgtttg tcagcggtga tgtaaacatt gtgagagata
      361 tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc aatacctttt
      421 aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct ggtcttgtag
      481 ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg catgacagac
      541 ttgaaaaagt catgttgttt catatgatct gggtatctag aaaaacattg aacaccccaa
      601 gtcagagtag tgactaaggt tggccatgga actggcaatt taccagtagt acaaataaat
      661 tttaaggtca atttaccgta cgtagcatca ccttcacctt caccggagac agaaaatttg
      721 tgaccattaa catcaccctc taattcaact aaaattggga cganaccgcc naataattct
      781 tcncctttag acatggnacc ngctttt
//
