LOCUS       sequence-250463-       10598 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of DDX3X S412F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-DDX3X-S412F
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    165..282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        330..1508
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          561..1499
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1456..1476
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1528..1552
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3545..3569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3577..3595
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3620..3639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3621..3639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3645..3665)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3719..3760
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3781..4348
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3798..4350
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3942..3959)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4169..4188
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4362..4364,4365..4367,4368..5081)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4362..4364,4365..4367,4368..5078)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4407..4428)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4668..4687)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5015..5036
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5095..5683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5148..5168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5566..5577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5758..5991
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6110..6128)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6196..6300
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6301..6369,6370..7161)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6519..6538)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7332..7920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7821..7840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8074..8091
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8331..8352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8367..8372,8373..8390,8391..8397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8405..8421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8410..8432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8429..8445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8429..8445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8495..8514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8706..9339
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9386..9511
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10008..10241
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10426..10470
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
       61 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
      121 tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
      181 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      241 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
      301 agcagagatc cagtttggac tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca
      361 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
      421 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      481 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      541 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
      601 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
      661 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
      721 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
      781 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
      841 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
      901 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
      961 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     1021 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     1081 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     1141 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     1201 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     1261 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     1321 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     1381 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     1441 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     1501 tgtcgtgaag cggccctgca gatatcaaca agtttgtaca aaaaagcagg caccatgagt
     1561 catgtggcag tggaaaatgc gctcgggctg gaccagcagt ttgctggcct agacctgaac
     1621 tcttcagata atcagagtgg aggaagtaca gccagcaaag ggcgctatat tcctcctcat
     1681 ttaaggaacc gagaagctac taaaggtttc tacgataaag acagttcagg gtggagttct
     1741 agcaaagata aggatgcgta tagcagtttt ggatctcgta gtgattcaag agggaagtct
     1801 agcttcttca gtgatcgtgg aagtggatca aggggaaggt ttgatgatcg tggacggagt
     1861 gattacgatg gcattggcag ccgtggtgac agaagtggct ttggcaaatt tgaacgtggt
     1921 ggaaacagtc gctggtgtga caaatcagat gaagatgatt ggtcaaaacc actcccacca
     1981 agtgaacgct tggaacagga actcttttct ggaggcaaca ctgggattaa ttttgagaaa
     2041 tacgatgaca ttccagttga ggcaacaggc aacaactgtc ctccacatat tgaaagtttc
     2101 agtgatgttg agatgggaga aattatcatg ggaaacattg agcttactcg ttatactcgc
     2161 ccaactccag tgcaaaagca tgctattcct attatcaaag agaaaagaga cttgatggct
     2221 tgtgcccaaa cagggtctgg aaaaactgca gcatttctgt tgcccatctt gagtcagatt
     2281 tattcagatg gtccaggcga ggctttgagg gccatgaagg aaaatggaag gtatgggcgc
     2341 cgcaaacaat acccaatctc cttggtatta gcaccaacga gagagttggc agtacagatc
     2401 tacgaggaag ccagaaaatt ttcataccga tctagagttc gtccttgcgt ggtttatggt
     2461 ggtgccgata ttggtcagca gattcgagac ttggaacgtg gatgccattt gttagtagcc
     2521 actccaggac gtctagtgga tatgatggaa agaggaaaga ttggattaga cttttgcaaa
     2581 tacttggtgt tagatgaagc tgatcggatg ttggatatgg ggtttgagcc tcagattcgt
     2641 agaatagtcg aacaagatac tatgcctcca aagggtgtcc gccacactat gatgtttagt
     2701 gctacttttc ctaaggaaat acagatgctg gctcgtgatt tcttagatga atatatcttc
     2761 ttggctgtag gaagagttgg ctctaccttt gaaaacatca cacagaaagt agtttgggtg
     2821 gaagaatcag acaaacggtc atttctgctt gacctcctaa atgcaacagg caaggattca
     2881 ctgaccttag tgtttgtgga gaccaaaaag ggtgcagatt ctctggagga tttcttatac
     2941 catgaaggat acgcatgtac cagcatccat ggagaccgtt ctcagaggga tagagaagag
     3001 gcccttcacc agttccgctc aggaaaaagc ccaattttag tggctacagc agtagcagca
     3061 agaggactgg acatttcaaa tgtgaaacat gttatcaatt ttgacttgcc aagtgatatt
     3121 gaagaatatg tacatcgtat tggtcgtacg ggacgtgtag gaaaccttgg cctggcaacc
     3181 tcattcttta acgagaggaa cataaatatt actaaggatt tgttggatct tcttgttgaa
     3241 gctaaacaag aagtgccgtc ttggttagaa aacatggctt atgaacacca ctacaagggt
     3301 agcagtcgtg gacgttctaa gagtagcaga tttagtggag ggtttggtgc cagagactac
     3361 cgacaaagta gcggtgccag cagttccagc ttcagcagca gccgcgcaag cagcagccgc
     3421 agtggcggag gtggccacgg tagcagcaga ggatttggtg gaggtggcta tggaggcttt
     3481 tacaacagtg atggatatgg aggaaattat aactcccagg gggttgactg gtggggtaac
     3541 tagaacccag ctttcttgta caaagtggtg atatccaatt aaccctcact aaaggtagag
     3601 aaagagagaa ttgtattaac cctatagtga gtcgtattac cacccaactt ttctatacaa
     3661 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     3721 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga
     3781 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3841 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3901 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3961 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4021 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4081 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4141 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4201 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4261 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4321 ggttttcctt tgaaaaacac gatgataata tggccacaca tatggtgagc aagggcgagg
     4381 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     4441 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     4501 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     4561 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     4621 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4681 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4741 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4801 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4861 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4921 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4981 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5041 ccgccgggat cactctcggc atggacgagc tgtacaagta aatcgataga tcctaatcaa
     5101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     5701 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5761 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     5821 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5881 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5941 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6001 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6061 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     6121 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6181 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6241 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6301 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6361 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6421 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6481 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6541 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6601 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6661 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6721 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6781 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6841 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6901 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6961 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7021 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7081 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7141 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7201 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7261 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7441 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7981 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8041 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8101 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     8161 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8221 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     8281 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8341 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8401 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     8461 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8521 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     8581 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     8641 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     8701 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     8761 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     8821 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8881 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8941 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     9001 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     9061 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     9121 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     9181 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9241 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9301 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     9361 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     9421 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     9481 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     9541 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     9601 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     9661 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     9721 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     9781 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     9841 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9901 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9961 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    10021 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    10081 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    10141 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    10201 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    10261 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    10321 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10381 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10441 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10561 gctgtacttt ctatagtgaa tagagttagg cagggata
//
