LOCUS       sequence-250527-       10786 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of BRAF A598_T599delinsA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-A598_T599delinsA
FEATURES             Location/Qualifiers
     source          1..10786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1389..1398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3690..3714)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3722..3740
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3765..3784)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3766..3784)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3790..3810)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3864..3905
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3926..4492
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3942..4494
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4086..4103)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4313..4332
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4506..5105
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4506..4525)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5002..5022
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5119..5707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5172..5192)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5590..5601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5782..6015
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6134..6152)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6220..6324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6325..6393,6394..7185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6543..6562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7356..7944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7845..7864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8098..8115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8355..8376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8391..8396,8397..8414,8415..8421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8429..8445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8434..8456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8453..8469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8453..8469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8519..8538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8730..9363
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9410..9535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10032..10265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10450..10494
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc
     1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc
     1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac
     1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat
     1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca
     1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat
     1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt
     1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt
     1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt
     1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt
     1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct
     2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt
     2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca
     2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc
     2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca
     2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc
     2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc
     2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt
     2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt
     2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg
     2581 tttgtctgct accccccctg cctcattacc tggctcacta actaacgtga aagccttaca
     2641 gaaatctcca ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg
     2701 aatgaaaaca cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat
     2761 tacagtggga caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca
     2821 tggtgatgtg gcagtgaaaa tgttgaatgt gacagcacct acacctcagc agttacaagc
     2881 cttcaaaaat gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat
     2941 gggctattcc acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt
     3001 gtatcaccat ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc
     3061 acgacagact gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct
     3121 caagagtaat aatatatttc ttcatgaaga cctcacagta aaaataggtg attttggtct
     3181 agcagtgaaa tctcgatgga gtgggtccca tcagtttgaa cagttgtctg gatccatttt
     3241 gtggatggca ccagaagtca tcagaatgca agataaaaat ccatacagct ttcagtcaga
     3301 tgtatatgca tttggaattg ttctgtatga attgatgact ggacagttac cttattcaaa
     3361 catcaacaac agggaccaga taatttttat ggtgggacga ggatacctgt ctccagatct
     3421 cagtaaggta cggagtaact gtccaaaagc catgaagaga ttaatggcag agtgcctcaa
     3481 aaagaaaaga gatgagagac cactctttcc ccaaattctc gcctctattg agctgctggc
     3541 ccgctcattg ccaaaaattc accgcagtgc atcagaaccc tccttgaatc gggctggttt
     3601 ccaaacagag gattttagtc tatatgcttg tgcttctcca aaaacaccca tccaggcagg
     3661 gggatatggt gcgtttcctg tccactagaa cccagctttc ttgtacaaag tggtgatatc
     3721 caattaaccc tcactaaagg tgagaatgta aagaatgttt attgccctat agtgagtcgt
     3781 attaccaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
     3841 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     3901 ctacgcgtac cggttagtaa tgagatccct ccccccccct aacgttactg gccgaagccg
     3961 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4021 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4081 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4141 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4201 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4261 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4321 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4381 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     4441 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     4501 cacatatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4561 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4621 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4681 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4741 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4801 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4861 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4921 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4981 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5041 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     5101 cctaaatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5161 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5221 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5281 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5341 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5401 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5461 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5521 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5581 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5641 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5701 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5761 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     5821 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5881 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5941 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6001 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6061 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
     6121 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6181 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6241 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6301 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6361 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6421 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6481 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6541 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     6601 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6661 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6721 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6781 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6841 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6901 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6961 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7021 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7081 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7141 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7201 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7261 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7321 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7381 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7441 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7501 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7561 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7621 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7681 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7741 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7801 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7861 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7921 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7981 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8041 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8101 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8161 tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg
     8221 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8281 taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt
     8341 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8401 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8461 agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8521 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     8581 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     8641 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     8701 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     8761 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     8821 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     8881 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     8941 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     9001 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     9061 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     9121 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     9181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9361 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     9421 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     9481 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     9541 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9601 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9661 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9721 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9781 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9841 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     9901 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     9961 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    10021 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    10081 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    10141 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    10201 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    10261 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    10321 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    10381 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    10441 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    10501 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    10561 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    10621 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    10681 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    10741 tctcgacggt atcgccgaat tcacaaatgg cagtattcat ccacaa
//
