LOCUS       sequence-250579-       12257 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of ERBB2 G776S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB2-G776S
FEATURES             Location/Qualifiers
     source          1..12257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7217..7241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7249..7267
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7292..7311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7293..7311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7317..7337)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7391..7432
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7453..8020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7470..8022
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7614..7631)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7841..7860
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8034..8633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8034..8053)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8530..8550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8647..9235
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8700..8720)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9118..9129)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9310..9543
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9662..9680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9748..9852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9853..9921,9922..10713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10071..10090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10884..11472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11373..11392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11626..11643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11883..11904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11919..11924,11925..11942,11943..11949)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11957..11973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11962..11984
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11981..11997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11981..11997
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12047..12066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagctggcgg ccttgtgccg ctgggggctc
     3481 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg
     3541 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag
     3601 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg
     3661 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg
     3721 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac
     3781 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg
     3841 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga
     3901 ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt gtggaaggac
     3961 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc
     4021 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat
     4081 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg
     4141 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac
     4201 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg
     4261 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc
     4321 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc
     4381 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt
     4441 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga
     4501 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt
     4561 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg
     4621 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac
     4681 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc
     4741 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc
     4801 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac
     4861 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa
     4921 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc
     4981 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc
     5041 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc
     5101 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat
     5161 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag
     5221 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg
     5281 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc
     5341 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg
     5401 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt
     5461 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg
     5521 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag
     5581 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt
     5641 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc
     5701 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca
     5761 tacgtgatgg ctagtgtggg ctccccatat gtctcccgcc ttctgggcat ctgcctgaca
     5821 tccacggtgc agctggtgac acagcttatg ccctatggct gcctcttaga ccatgtccgg
     5881 gaaaaccgcg gacgcctggg ctcccaggac ctgctgaact ggtgtatgca gattgccaag
     5941 gggatgagct acctggagga tgtgcggctc gtacacaggg acttggccgc tcggaacgtg
     6001 ctggtcaaga gtcccaacca tgtcaaaatt acagacttcg ggctggctcg gctgctggac
     6061 attgacgaga cagagtacca tgcagatggg ggcaaggtgc ccatcaagtg gatggcgctg
     6121 gagtccattc tccgccggcg gttcacccac cagagtgatg tgtggagtta tggtgtgact
     6181 gtgtgggagc tgatgacttt tggggccaaa ccttacgatg ggatcccagc ccgggagatc
     6241 cctgacctgc tggaaaaggg ggagcggctg ccccagcccc ccatctgcac cattgatgtc
     6301 tacatgatca tggtcaaatg ttggatgatt gactctgaat gtcggccaag attccgggag
     6361 ttggtgtctg aattctcccg catggccagg gacccccagc gctttgtggt catccagaat
     6421 gaggacttgg gcccagccag tcccttggac agcaccttct accgctcact gctggaggac
     6481 gatgacatgg gggacctggt ggatgctgag gagtatctgg taccccagca gggcttcttc
     6541 tgtccagacc ctgccccggg cgctgggggc atggtccacc acaggcaccg cagctcatct
     6601 accaggagtg gcggtgggga cctgacacta gggctggagc cctctgaaga ggaggccccc
     6661 aggtctccac tggcaccctc cgaaggggct ggctccgatg tatttgatgg tgacctggga
     6721 atgggggcag ccaaggggct gcaaagcctc cccacacatg accccagccc tctacagcgg
     6781 tacagtgagg accccacagt acccctgccc tctgagactg atggctacgt tgcccccctg
     6841 acctgcagcc cccagcctga atatgtgaac cagccagatg ttcggcccca gcccccttcg
     6901 ccccgagagg gccctctgcc tgctgcccga cctgctggtg ccactctgga aaggcccaag
     6961 actctctccc cagggaagaa tggggtcgtc aaagacgttt ttgcctttgg gggtgccgtg
     7021 gagaaccccg agtacttgac accccaggga ggagctgccc ctcagcccca ccctcctcct
     7081 gccttcagcc cagccttcga caacctctat tactgggacc aggacccacc agagcggggg
     7141 gctccaccca gcaccttcaa agggacacct acggcagaga acccagagta cctgggtctg
     7201 gacgtgccag tgtagaaccc agctttcttg tacaaagtgg tgatatccaa ttaaccctca
     7261 ctaaaggatg aaagatttag ttgtgagata tccctatagt gagtcgtatt accacccaac
     7321 ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
     7381 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     7441 ttagtaatga gatccctccc ccccccntaa cgttactggc cgaagccgct tggaataagg
     7501 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     7561 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7621 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7681 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     7741 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     7801 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     7861 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     7921 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     7981 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg
     8041 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     8101 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     8161 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8221 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     8281 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8341 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8401 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8461 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8521 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     8581 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata
     8641 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8701 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8761 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8821 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8881 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8941 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9001 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9061 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9121 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9181 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     9241 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     9301 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     9361 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9541 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     9601 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     9661 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     9721 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9841 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9901 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9961 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10021 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10081 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10141 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10201 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10261 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10321 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10381 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10441 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10501 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10561 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10621 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10681 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10741 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10801 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11641 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11701 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    11761 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    11821 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    11881 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    11941 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    12001 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    12061 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    12121 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    12181 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    12241 actacaaact tagtagt
//
