LOCUS       sequence-251501-        1338 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
  TITLE     Engineering Strategy and Vector Library for the Rapid Generation of 
            Modular Light-Controlled Protein-Protein Interactions.
  JOURNAL   J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033.
  PUBMED    31150735
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033."
COMMENT     Sequence Label: Opto-casp9_casp9-VfAU1-LOV
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(343..354)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accatggcca ccgggggatt tggtgatgtc ggtgctcttg agagtttgag gggaaatgca
       61 gatttggctt acatcctgag catggagccc tgtggccact gcctcattat caacaatgtg
      121 aacttctgcc gtgagtccgg gctccgcacc cgcactggct ccaacatcga ctgtgagaag
      181 ttgcggcgtc gcttctcctc gctgcatttc atggtggagg tgaagggcga cctgactgcc
      241 aagaaaatgg tgctggcttt gctggagctg gcgcggcagg accacggtgc tctggactgc
      301 tgcgtggtgg tcattctctc tcacggctgt caggccagcc acctgcagtt cccaggggct
      361 gtctacggca cagatggatg ccctgtgtcg gtcgagaaga ttgtgaacat cttcaatggg
      421 accagctgcc ccagcctggg agggaagccc aagctctttt tcatccaggc ctgtggtggg
      481 gagcagaaag accatgggtt tgaggtggcc tccacttccc ctgaagacga gtcccctggc
      541 agtaaccccg agccagatgc caccccgttc caggaaggtt tgaggacctt cgaccagctg
      601 gacgccatat ctagtttgcc cacacccagt gacatctttg tgtcctactc tactttccca
      661 ggttttgttt cctggaggga ccccaagagt ggctcctggt acgttgagac cctggacgac
      721 atctttgagc agtgggctca ctctgaagac ctgcagtccc tcctgcttag ggtcgctaat
      781 gctgtttcgg tgaaagggat ttataaacag atgcctggtt gctttaattt cctccggaaa
      841 aaacttttct ttaaaacatc acccggtgaa ttctcaggct ctggatcccc cggtcctgac
      901 tacagtctcg tgaaggctct gcaaatggca caacagaatt ttgtcattac agacgcctcc
      961 ctcccagaca accctatcgt ctacgccagt agagggtttc tgacactgac aggctattct
     1021 ctcgaccaga tcctgggcag gaactgcagg tttctgcaag ggccagaaac agacccaaga
     1081 gctgtggata agatcaggaa tgccatcacc aaaggcgttg ataccagtgt ctgtctgctg
     1141 aattatagac aggatggcac aaccttctgg aatctcttct tcgtggctgg actcagagat
     1201 tctaagggca atattgtcaa ctacgtcgga gtgcagtcaa aggtgagcga agattatgcc
     1261 aagctgctgg tcaacgagca gaacattgag tacaaaggtg tgcgcaccag taacatgctg
     1321 cgcagaaagc ccgggtaa
//
