LOCUS       sequence-251729-        9976 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Inducible expression of Ptp69D-GFP in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT Ptp69D-GFP
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..172
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     164..181
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     165..181
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        187..613
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             648..665
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5139..5852
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             5139..5849
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5166..5194)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5789..5809
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(5872..5889)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5891..6025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             6500..7099
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6500..6519)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6996..7016
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7232..7248)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7233..7249)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    7264..7398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7541..7557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7541..7557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7554..7576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7565..7581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7589..7595,7596..7613,7614..7619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7634..7655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7772..7789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7943..8531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8023..8042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8702..9493,9494..9562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9325..9344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9563..9667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9735..9753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9791..9813)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9913..9932
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      181 tgaattaatt cgttgcagga caggatgtgg tgcccgatgt gactagctct ttgctgcagg
      241 ccgtcctatc ctctggttcc gataagagac ccagaactcc ggccccccac cgcccaccgc
      301 cacccccata catatgtggt acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac
      361 caagagcttt gcatcccata caagtcccca aagtggagaa ccgaaccaat tcttcgcggg
      421 cagaacaaaa gcttctgcac acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa
      481 agaggtgaat cgaacgaaag acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg
      541 agagcatctg gccaatgtgc atcagttgtg gtcagcagca aaatcaagtg aatcatctca
      601 gtgcaactaa aggggggatc tagatcgggg tacctactag tgaaatgcac catcaccatc
      661 atcacgaaga tcaagttgac ccaagactta ttgacggaaa aggaggagga ggccggccca
      721 tggcgttact ctaccgacgc atgtcgatgc tattaaatat aattcttgca tatattttcc
      781 tttgtgcgat atgtgtgcaa ggatctgtga aacaagagtg ggcagaaata ggtaaaaacg
      841 tatctctaga gtgcgcatct gaaaacgaag cagtatcatg gaaactgggc aaccaaacta
      901 ttaataaaaa ccatacaaga tataaaataa gaactgagcc attgaaatcc aacgacgatg
      961 gcagtgaaaa caacgatagt caagacttca tcaagtataa gaatgtcctg acgcttctcg
     1021 atgtcaatat aaatgactcg gggaactaca cctgcacagc ccaaacgggg caaaaccatt
     1081 cgactgaatt ccaagttagg ccataccttc catccaaagt cctgcaaagt acccccgata
     1141 ggattaagag aaaaatcaag caggatgtca tgttgtactg tttgatcgag atgtacccac
     1201 aaaatgagac aactaataga aacctcaagt ggcttaagga cggcagccag tttgaattcc
     1261 tggacacctt ctcgtccatt tcgaagctaa acgatacgca cttaaacttc accctggaat
     1321 tcacagaagt ttacaagaaa gagaatggca cctacaagtg caccgtcttc gatgacaccg
     1381 gactcgaaat tacctccaaa gagattaccc ttttcgtaat ggaagtgcca caagttagca
     1441 ttgatttcgc caaggcagtt ggtgctaata aaatatacct aaattggacc gttaacgacg
     1501 gcaacgatcc aattcagaag ttcttcatca ctctgcagga ggctggaaca ccgactttta
     1561 cttatcataa ggactttatt aacggcagcc atacatcgta tattctggac catttcaagc
     1621 cgaacacaac ctatttttta agaatcgtgg gaaagaactc gattggcaat ggccaaccca
     1681 cccagtatcc ccagggaatc accacgctta gttatgatcc catatttata ccaaaagtcg
     1741 agaccaccgg cagcacggcg tccacgataa cgattggctg gaatccaccg ccgccagatc
     1801 tcattgatta tatacaatac tacgaactga ttgtctccga atcgggcgag gtgcccaaag
     1861 tgatcgaaga ggccatttac cagcagaatt ctcgaaactt gccatacatg tttgataagc
     1921 ttaagaccgc cacagactac gaatttaggg taagggcatg tagtgatcta accaagactt
     1981 gtggaccatg gtccgaaaac gtgaacggca caacgatgga cggcgtggca acaaaaccca
     2041 ccaacttgag catacagtgt catcatgaca acgtcacgag aggcaactcc atcgccatta
     2101 actgggacgt tcccaagacg ccaaacggca aggttgtgtc atatttaatt cacttgctgg
     2161 gcaatcccat gagcacagtg gatagagaga tgtggggacc aaagattcga agaatcgatg
     2221 aaccccatca caagaccctc tacgaaagtg ttagcccgaa cacaaactac acggtgacgg
     2281 tgtctgccat aacgcggcac aagaagaacg gcgaaccggc caccggaagt tgtctaatgc
     2341 ccgtctccac gccggatgcc attggccgta ccatgtggtc aaaggtgaac ctggactcca
     2401 agtacgttct caagttgtat ctaccgaaga tcagcgaacg caatgggccc atatgctgct
     2461 atagattgta tctagttaga attaacaatg acaacaagga attgccggac ccggagaagc
     2521 taaacatcgc cacatatcag gaggttcaca gcgataacgt gactagaagt agtgcatata
     2581 tagcggaaat gataagtagc aagtacttta ggccggaaat attcttgggc gatgaaaaga
     2641 gatttagtga gaacaacgat ataatccgcg acaacgacga aatttgtcgg aaatgcttgg
     2701 agggtactcc atttctgagg aaacccgaga tcattcacat acccccacaa ggttcactat
     2761 caaattccga ttctgaactg cccattttgt cggagaagga caacttgatc aaaggagcaa
     2821 acttaacaga gcatgctctg aaaatcttag aaagtaagtt aagggataaa agaaacgcgg
     2881 tgaccagcga tgagaatcca attctaagcg ccgtcaaccc aaatgtgcca ctccacgatt
     2941 ctagtcgaga tgtcttcgat ggtgagatag atattaactc caactacacc ggattcctag
     3001 agataatagt tcgggatcga aacaatgccc tgatggctta tagcaaatac tttgatataa
     3061 ttactccggc gactgaagct gaacccatcc aatccttgaa caatatggac tactacctaa
     3121 gcattggggt caaggctgga gccgtattac ttggtgtcat acttgtattt attgtgctgt
     3181 gggttttcca tcacaagaaa accaagaatg aactgcaggg cgaagacact ttaacactaa
     3241 gagattcttt gagggcattg ttcggtcgac gaaatcacaa ccacagtcat tttataactt
     3301 ccggaaatca caaaggattc gacgctggcc ccattcacag attagattta gaaaacgcct
     3361 acaagaaccg acataaggac actgactacg gatttctgcg ggaatatgag atgctgccaa
     3421 atcgctttag cgatcggaca actaaaaaca gtgatttaaa ggagaacgcc tgcaagaaca
     3481 ggtatcccga tataaaggct tacgatcaga cgcgcgtaaa gttggctgtc atcaatggcc
     3541 tgcaaactac ggactacatc aatgctaact tcgtaattgg ctacaaggag agaaagaagt
     3601 ttatctgtgc acagggtcca atggaaagta ccatcgatga tttttggcga atgatttggg
     3661 aacaacatct agaaataatt gtgatgctta caaatttgga ggaatataac aaggccaagt
     3721 gcgcgaaata ttggccagaa aaagtatttg atacgaaaca gttcggagat attttagtga
     3781 aatttgcaca agaacgtaag actggtgatt atattgaacg taccctgaac gtttccaaga
     3841 acaaagccaa tgtcggcgag gaggaggacc gtagacaaat cacccaatac cactacctaa
     3901 cgtggaagga cttcatggca ccagagcatc cacatggcat catcaaattc atacgtcaaa
     3961 tcaattccgt ctactccctg caaaggggtc caattttagt gcattgcagt gctggtgtgg
     4021 gtagaaccgg aaccctggtg gctttagatt ccctaatcca acagctggag gaagaagact
     4081 cggtgtccat ttacaacaca gtgtgtgatc tacgacacca acgaaatttt ttagtccaat
     4141 ctctgaaaca atacatcttt ctttaccggg ctttattaga tactggaacc tttggaaaca
     4201 cggatatttg cattgataca atggcttctg caattgaatc tcttaagcgg aaacccaacg
     4261 agggtaaatg caaattggaa gtggaattcg agaaactact ggccacagca gatgagataa
     4321 gtaaatcatg tagtgtgggc gaaaacgagg agaacaatat gaagaacaga agtcaagaga
     4381 ttatacccta cgatcgcaac agagttatac taacaccact tccgatgcgg gaaaactcaa
     4441 cttacattaa cgcatcattc atagagggct atgataatag cgaaaccttc atcattgccc
     4501 aggatccact tgaaaacact ataggagact tctggcgaat gatctcggaa cagagtgtta
     4561 ccaccctcgt catgatatcc gaaatcggag atggtcccag gaaatgcccg cgatactggg
     4621 cagatgatga ggttcaatac gatcacatac tcgtgaaata cgtgcacagc gaaagttgtc
     4681 catactatac tcgccgcgaa ttttatgtta cgaattgcaa aatagacgat acgctgaaag
     4741 tcacacaatt tcaatacaat ggttggccca ccgtggacgg agaagttcct gaagtctgcc
     4801 gtggcattat agaacttgta gatcaagcat acaaccatta taagaacaac aagaattctg
     4861 gttgtcgatc tccactcaca gttcattgca gcctgggaac cgatcgaagt tccattttcg
     4921 tcgccatgtg tattttggtc cagcatctga ggctggagaa gtgtgtcgac atctgtgcca
     4981 caacaaggaa attacgatct cagcgaacgg gacttatcaa ctcatacgca caatacgagt
     5041 tcctacatcg cgcaataatt aattattcag acttacatca catagccgag tcaacattgg
     5101 atgggcgcgc cggcggccgc tcgagtctag agggccccag caaaggagaa gaacttttca
     5161 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     5221 tccgtggaga gggtgaaggt gatgctacaa acggaaaact cacccttaaa tttatttgca
     5281 ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactctgacc tatggtgttc
     5341 aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc
     5401 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc
     5461 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg
     5521 attttaaaga agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca
     5581 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
     5641 acaacgttga agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg
     5701 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga
     5761 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
     5821 ttacacatgg catggatgag ctctacaaat aggtttaaac ccgctgatca gcctcgactg
     5881 tgccttctaa gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     5941 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6001 cattataagc tgcaataaac aagttaactc tagaggatcc ggtgcgtggt ggtttcatgc
     6061 ttctgggaac ggcaaatggg tttaggattg ggaacccctc atcatctgtt ggaatatact
     6121 attcaaccta caaaagtaac gttaaacaac actactttat atttgatatg aatggccaca
     6181 ccttttatgc cataaaacat attgtaagag aataccactc tttttattcc ttctttcctt
     6241 cttgtacgtt ttttgctgta agtaggtcgt ggtgctggtg ttgcagttga aataacttaa
     6301 aatataaatc ataaaactca aacataaact tgactattta tttatttatt aagaaaggaa
     6361 atataaatta taaattacaa cagatccccc gggctgcagg aattcgatag cttgcatgcc
     6421 tgcaggtcgg ccgccacgac cggtgccgcc accatcccct gacccacgcc cctgacccct
     6481 cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6541 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6601 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6661 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6721 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6781 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6841 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6901 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6961 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7021 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7081 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     7141 acgaccccat ggctccgacc gaagccaccc ggggcggccc cgccgacccc gcacccgccc
     7201 ccgaggccca ccgactctag atcaagctta tcgataccgt cgacctcgag gccagctagc
     7261 ttgaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7321 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7381 tcttatcatg tctggatcgg gtacccaatt gcattcattt tatgtttcag gttcaggggg
     7441 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     7501 atcagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7621 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7681 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9661 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9721 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9781 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9841 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9901 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9961 taccgcacag atgcgt
//
