LOCUS       sequence-251929-        3902 bp    DNA     circular SYN 14-APR-2025
DEFINITION  asymmetric plasmid partitioning E. coli - target plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pAA3
FEATURES             Location/Qualifiers
     source          1..3902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             281..955
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1138..1165
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             1597..1788
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1782..1804)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1890..2029
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1945..1964
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2044..2061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2215..2803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2295..2314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2852..3511)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3426..3445
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(3825..3846)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaagaaga
      121 caaaaatcac cttgcgctaa gagctcgaca ccatcgaatg gtgcaaaacc tttcgcggta
      181 tggcatgata gcgcccggaa gcatgccact taaaaaggag atcaacaatg aaagcaattt
      241 tcgtactgaa acatcttaat catgctgggg agggtttcta atggcttcct ccgaagacgt
      301 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      361 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      421 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      481 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      541 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      601 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      661 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      721 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      781 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      841 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      901 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgctacctc
      961 cgcagcgaac gacgaaaatt acgcccttgc agcgtaataa ctacgtgctg ctgaagttgc
     1021 ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc
     1081 atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc tcacactggc
     1141 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat
     1201 tattaatccg gcttttttat tatttcctag ctagccaacg catatggcaa aacgtctgcg
     1261 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg
     1321 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc
     1381 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc
     1441 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
     1501 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
     1561 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc
     1621 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag
     1681 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt
     1741 taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     1801 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     1861 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     1921 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt
     1981 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     2041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2821 ttctacgggg tctgacagct tggtctgaca gttacgcccc gccctgccac tcatcgcagt
     2881 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     2941 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatcgtga
     3001 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     3061 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     3121 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     3181 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     3241 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
     3301 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     3361 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     3421 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     3481 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     3541 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     3601 ttacgtgccc gatcaactta ccaatgctgt atttagaaaa ataaacaaat aggggttccg
     3661 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3721 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3781 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3841 gtccacctcg agctttcagg aaaatttttc tgtataatag attcataaat ttgagagaag
     3901 ag
//
