LOCUS       sequence-252004-       10219 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs,
            crRNAs, shRNAs and etc...).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W,
            Deem A, Bristow C, Hart T, Draetta G
  TITLE     Pooled library screening with multiplexed Cpf1 library.
  JOURNAL   Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x.
  PUBMED    31316073
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-17"; volume: "10(1):3144. doi"; pages: " 
            10.1038/s41467-019-10963-x"
COMMENT     Sequence Label: LentiUniversal-Puro
FEATURES             Location/Qualifiers
     source          1..10219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1713..1733
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1884..1903
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3850..3925
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4010..4127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4266..5444
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4497..5435
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5392..5412
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5457..6056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5457..5476)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5953..5973
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6072..6660
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6125..6145)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6543..6554)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6732..6965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7043..7164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7080..7099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7134..7153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7204..7339
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7359..7378)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7360..7378)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7388..7405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7388..7404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7397..7419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7546..8001
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7633..7652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7843..7864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8027..8131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8132..8200,8201..8992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8350..8369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9163..9751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9652..9671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9905..9922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10039..10060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10075..10080,10081..10098,10099..10105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10113..10129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10118..10140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10137..10153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10137..10153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10172..10192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10174..10192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     1741 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     1801 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1861 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     1921 atttcttggc tttatatatc ttgtggggag acgaaaggac gaaacaccgg agacggttgt
     1981 aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa
     2041 ccaaaatctt ctttttaata actttagtat caataattag aatttttatg ttcctttttg
     2101 caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt
     2161 ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc
     2221 aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt
     2281 gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa
     2341 tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat
     2401 gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc
     2461 tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc
     2521 accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt cagaaacttc
     2581 gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat ttgttattcc
     2641 tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat taacacaaac
     2701 tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag ctttcgtagc
     2761 atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt ctcctatata
     2821 ttcattttta attttaattc tttaataatt tcgtctactt taactttagc gttttgaaca
     2881 gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact tgggcgaaca
     2941 gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg catattatac
     3001 attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg agttaagggt
     3061 gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc tggtttaaaa
     3121 ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc ttctaaatag
     3181 tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat taatatagaa
     3241 gcttgtattc catctttatc tctagctgag tcatcaatta catatccata actttcttca
     3301 taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct acccattcat
     3361 ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc gattctatca
     3421 cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat gctatttaag
     3481 cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt tccatctaat
     3541 cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc tgggtcattc
     3601 ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt tcatgcagga
     3661 tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa tgcatgctct
     3721 tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa tttagttcct
     3781 gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg ctcttttttg
     3841 tacgtctctg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     3901 tgaaaaagtg gcaccgagtc ggtgcttttt taattaagga tcccctcggc gtctctggtt
     3961 aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     4021 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     4081 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     4141 gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa
     4201 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     4261 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     4321 tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg
     4381 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     4441 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     4501 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     4561 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     4621 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     4681 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     4741 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     4801 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     4861 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     4921 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     4981 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     5041 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     5101 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     5161 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     5221 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     5281 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     5341 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     5401 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg gccaccatga
     5461 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5521 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5581 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5641 gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg
     5701 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5761 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5821 cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5881 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5941 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6001 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaacgc
     6061 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6121 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6181 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6241 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6301 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6361 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6421 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6481 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6541 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6601 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6661 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6721 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6781 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6841 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6901 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6961 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7021 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7081 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7141 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7201 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7261 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7321 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7381 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7441 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7501 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7981 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8341 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8641 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9301 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9781 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9841 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9901 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9961 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    10021 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    10081 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    10141 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
    10201 gctggagctg caagcttaa
//
