LOCUS       sequence-252160-        2800 bp    DNA     circular SYN 14-APR-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: gfasPurple chromoprotein
FEATURES             Location/Qualifiers
     source          1..2800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    676..696
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      697..754
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(778..795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(949..1537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1029..1048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1720..1814)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1835..2494)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2409..2428
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2656..2674
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2678..2721)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2724..2745
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2746..2780
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             2789..2800
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tactagatgt cggtgattgc taaacagatg acctacaaag tctatatgtc gggtacggtg
       61 aacggccatt attttgaagt tgaaggtgac ggtaaaggca agccgtatga aggcgaacag
      121 accgttaaac tgaccgtcac gaagggcggt ccgctgccgt ttgcatggga tattctgagt
      181 ccgcagtccc aatatggcag catcccgttc acgaaatatc cggaagatat cccggactac
      241 gtgaagcagt cttttccgga aggttacacc tgggaacgta tcatgaactt cgaagatggc
      301 gccgtctgca ccgtgagtaa cgacagctct attcaaggta attgtttcat ctaccatgtc
      361 aagttctcag gtctgaactt cccgccgaat ggcccggtga tgcagaaaaa gacccaaggc
      421 tgggaaccga atacggaacg tctgtttgca cgcgatggta tgctgattgg caacaatttc
      481 atggctctga aactggaagg cggtggccac tatctgtgcg aatttaaaag cacctacaag
      541 gcgaaaaagc cggttaaaat gccgggctat cattacgtgg atcgtaaact ggacgttacc
      601 aaccacaata aggactatac gtccgtcgaa cagtgtgaaa tttcaatcgc gcgcaaatcg
      661 gtggttgcct aataatacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
      721 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1681 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1741 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1801 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1861 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1921 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1981 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     2041 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     2101 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2161 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2221 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2281 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2341 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2401 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2461 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2521 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2581 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     2641 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2701 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagtttac ggctagctca
     2761 gtcctaggta caatgctagc tactagagaa agaggagaaa
//
