LOCUS       sequence-252174-        2845 bp    DNA     circular SYN 14-APR-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: fwYellow chromoprotein
FEATURES             Location/Qualifiers
     source          1..2845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     misc_feature    844..864
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      865..922
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(946..963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1117..1705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1197..1216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1888..1982)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2003..2662)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2577..2596
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2824..2842
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaccttagga
      121 ggtaaacata tgacggcact gactgaaggc gcaaaactgt tcgagaaaga aatcccatat
      181 atcactgagc tggaaggtga cgttgaaggt atgaagttta tcatcaaggg tgaaggtacc
      241 ggtgacgcga gcgtcggtaa agtggatgct cagttcattt gtaccacggg cgacgttccg
      301 gttccgtgga gcacgctggt caccacgctg acgtatggtg ctcagtgctt tgccaagtat
      361 ccgcgccaca ttgcggattt cttcaaaagc tgcatgccgg aaggttacgt ccaagagcgc
      421 accatcacct ttgagggtga tggcgtgttc aagacccgtg cggaagtcac ctttgaaaat
      481 ggcagcgtgt acaaccgtgt aaaactgaac ggccagggtt tcaagaagga cggccacgtg
      541 ctgggcaaaa atctggagtt taactttacc cctcattgtt tgtacatttg gggtgaccaa
      601 gcgaatcatg gcctgaagag cgcgttcaaa atcatgcatg agatcaccgg ctccaaagag
      661 gatttcattg ttgccgatca cacccaaatg aataccccga ttggtggtgg tccggtgcac
      721 gtgccggagt accaccacat tacgtatcat gttaccctgt ctaaagacgt caccgatcac
      781 cgtgaccatt tgaacattgt tgaggtgatc aaggcagttg acctggagac gtaccgttaa
      841 taatactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc
      901 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc
      961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
     1141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1861 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     1921 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1981 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     2041 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     2101 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     2161 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     2221 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     2281 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2341 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2401 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     2461 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     2521 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2581 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2641 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     2701 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     2761 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2821 aaaaataggc gtatcacgag gcaga
//
