LOCUS       sequence-252182-        9662 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Yeast integrative vector for expression of the SUMO-SIM complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Delarue M, Brittingham GP, Pfeffer S, Surovtsev IV, Pinglay S, 
            Kennedy KJ, Schaffer M, Gutierrez JI, Sang D, Poterewicz G, Chung 
            JK, Plitzko JM, Groves JT, Jacobs-Wagner C, Engel BD, Holt LJ
  TITLE     mTORC1 Controls Phase Separation and the Biophysical Properties of 
            the Cytoplasm by Tuning Crowding.
  JOURNAL   Cell. 2018 Jul 12;174(2):338-349.e20. doi: 
            10.1016/j.cell.2018.05.042. Epub 2018 Jun 21.
  PUBMED    29937223
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.042"; journalName: "Cell"; date: 
            "2018-07-12- 12"; volume: "174"; issue: "2"; pages: "338-349.e20"
COMMENT     Sequence Label: pAV106-pTDH3-mCherry-10xSumo-6xSIM
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2243..2261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2308..2974
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2844..2867
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             3026..3052
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3110..3817
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3255..3273)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3523..3542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3717..3740
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7681..7700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7682..7700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7707..7724)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7707..7723)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7716..7738)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7864..8319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7951..7970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8161..8182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8450..9253)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9254..9469)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9493..9512)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9612..9634
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg
     2281 gccccccctc gagtcagttc gagtttatca ttatcaatac tgccatttca aagaatacgt
     2341 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
     2401 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt
     2461 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
     2521 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc
     2581 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa
     2641 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg
     2701 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc
     2761 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta
     2821 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct
     2881 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa
     2941 cttagtttcg aataaacaca cataaacaaa caaattaatt aaaaaaatgt ctttgatcac
     3001 aagtggagcg tcaggggcac ttcagttgcc accgttggaa agactaactc tgggctccgc
     3061 tggatcagct ttagccttga aattagcagg tcttgatatc ctgatcacta tggtgagcaa
     3121 gggcgaggag gacaacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3181 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3241 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     3301 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3361 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3421 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3481 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3541 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     3601 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
     3661 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagct
     3721 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
     3781 ccgccactcc accggcggca tggacgagct gtacaaggcg gccgcaaccg gttcatccgg
     3841 atcttcaggg agttctggtt caagcatgtc agaagagaag cctaaggagg gcgtgaaaac
     3901 cgagaatgac cacattaact tgaaggttgc tggacaggat gggtccgtgg ttcagttcaa
     3961 aattaaacgc cacacaccgt tgtcgaaact gatgaaagcg tacagcgaac gccagggtct
     4021 ttctatgcgt cagattcgct tccgctttga tggtcaaccg attaatgaaa cggacacgcc
     4081 ggcacagctg gagatggagg atgaagacac tatcgacgta tttcagcagc agaccgtcgt
     4141 agggggctcc ggcggaagcg gggggtcggg aggttccatg agcgaagaga aaccaaaaga
     4201 aggagttaaa accgaaaacg accatatcaa cctgaaggtc gccggtcagg acggctcagt
     4261 cgtgcagttc aagattaaac gtcatacccc attaagtaag ctgatgaagg cgtatagtga
     4321 gcgccaaggc ttgtcgatgc gccagattcg ttttcgcttt gatggtcagc caatcaacga
     4381 aaccgacacc ccggcgcagc tggaaatgga agatgaagat accatcgatg tgttccaaca
     4441 gcaaacggtt gttggcggaa gcggcgggag cggtggtagc gggggttcta tgtccgaaga
     4501 aaaaccgaaa gagggtgtga agactgaaaa cgaccacatt aatctgaagg tggcgggcca
     4561 ggacggttcc gttgttcagt ttaaaatcaa acgtcacacc ccgctgtcga aactgatgaa
     4621 agcctatagc gaacgtcaag ggctgagcat gcgtcaaatt cgttttcgct ttgatggtca
     4681 gccaatcaac gaaaccgata caccggcgca gctggagatg gaagacgagg acaccattga
     4741 cgtcttccag caacaaactg tagtgggtgg ctccggtggc tctggtgggt ctggtggctc
     4801 gatgagtgag gaaaaaccca aagaaggcgt caagactgag aacgatcata ttaacctgaa
     4861 agtcgcgggt caagatggtt cagtggtgca gttcaaaatt aaacgccaca ccccgctgtc
     4921 aaagctgatg aaagcctata gtgagcgtca aggtctgtcg atgcgccaga tccgctttcg
     4981 ctttgatggt cagccaatca acgagacaga cactccggca caactggaga tggaagatga
     5041 agacaccatc gatgtttttc aacagcagac cgtggtggga gggagcggcg gtagcggggg
     5101 atcaggtggt agtatgtctg aggaaaagcc gaaggaaggg gtaaagaccg aaaatgatca
     5161 cattaatctg aaagttgcgg gccaagatgg tagtgtagtg caatttaaaa tcaagcgtca
     5221 caccccgtta tctaaactga tgaaagcata tagcgaacgc caaggcctgt ctatgcgcca
     5281 gattcgcttt cgctttgacg ggcaaccaat taatgaaacg gacaccccgg cacaactgga
     5341 aatggaagac gaggatacta ttgacgtgtt tcagcaacag acagtcgtag gttcatccgg
     5401 atcttcaggg agttctggtt caagcatgag cgaagagaaa cctaaagaag gagttaaaac
     5461 cgaaaatgac catatcaacc tgaaggtggc cggccaggac ggctcagtcg tgcagttcaa
     5521 gatcaaacgt cataccccat taagtaagct gatgaaggcg tatagtgagc gccaaggctt
     5581 gtcgatgcgc cagattcgtt tccgctttga cggtcagcca atcaacgaaa ccgacacccc
     5641 ggcgcagctg gaaatggaag acgaagatac tatcgatgtg ttccaacagc aaacggtcgt
     5701 aggatcttca ggatcaagcg gttcatctgg tagttccaga tctatgtcag aagagaagcc
     5761 taaggaaggc gtgaaaaccg agaatgacca cattaacttg aaggttgctg gacaggatgg
     5821 gtccgtggtt cagttcaaga ttaaacgcca tacaccgttg tcgaaactga tgaaggcgta
     5881 cagcgaacgt cagggtcttt ctatgcgtca gattcgcttc cgctttgatg gtcaaccgat
     5941 taatgaaacg gacacgccgg cacagctgga gatggaggat gaggacacta tcgacgtatt
     6001 tcagcaacag accgttgttg gatcttcagg atcaagcggt tcatctggta gttccatgtc
     6061 cgaagaaaaa ccgaaggagg gtgtgaagac tgaaaacgac catattaatc tgaaggtggc
     6121 gggccaggac ggttccgttg ttcagtttaa gatcaaacgc cacaccccgc tgtcgaaact
     6181 gatgaaagcc tatagtgaac gtcaagggct gagcatgcgt caaattcgtt ttcgctttga
     6241 tggtcaacca atcaacgaaa ccgatacacc ggcgcagctg gaaatggaag acgaggacac
     6301 cattgacgtc ttccagcaac aaactgttgt tggttcctct ggaagctcag gatctagtgg
     6361 gtcatcaact agtatgagtg aagaaaaacc caaggaaggc gtcaagactg agaacgatca
     6421 tattaacctg aaagtcgcgg gtcaagatgg ttcagtggtg cagtttaaaa ttaaacgcca
     6481 caccccgttg tcaaagctga tgaaagccta tagtgaacgt caaggtctgt cgatgcgcca
     6541 gatccgcttc cgctttgatg gtcagccaat caacgagaca gacactccgg cacaactgga
     6601 gatggaagat gaagacacca tcgatgtttt ccaacagcag accgttgttg gttcatcagg
     6661 ttcatccgga tctagcgggt caagtatgtc tgaagaaaag ccgaaggaag gggtaaagac
     6721 cgaaaatgat cacattaatc tgaaagttgc gggccaggat ggtagtgtag tgcaatttaa
     6781 aattaaacgt cacaccccgt tgtctaaact gatgaaagca tatagcgaac gccaaggcct
     6841 gtctatgcgc cagattcgct ttcgctttga cgggcaacca atcaatgaaa cggacacccc
     6901 ggcacaactg gaaatggaag acgaggacac tattgacgtg tttcagcaac agacaggttc
     6961 atccggatct tcagggagtt ctggttcaag caaggttgat gtaatagatt tgaccataga
     7021 atctagttct gacgaggaag aagatcctcc tgcaaagagg ggatcttcag gatcaagcgg
     7081 ttcatctggt agttcctcta gaaaggttga tgtaatagat ttgaccatag aatctagttc
     7141 tgacgaggaa gaagatcctc ctgcaaagag gggtggttcc gggggttcag ggggttctgg
     7201 tggaagcgga tccaaggttg atgtaataga tttgaccata gaatctagtt ctgacgagga
     7261 agaagatcct cctgcaaaga ggggttcctc tggaagctca ggatctagtg ggtcatcaaa
     7321 ggttgatgta atagatttga ccatagaatc tagttctgac gaggaagaag atcctcctgc
     7381 aaagaggggt ggttccgggg gttcaggggg ttctggtgga agcaaggttg atgtaataga
     7441 tttgaccata gaatctagtt ctgacgagga agaagatcct cctgcaaaga ggggtggttc
     7501 cgggggttca gggggttctg gtggaagcaa ggttgatgta atagatttga ccatagaatc
     7561 tagttctgac gaggaagaag atcctcctgc aaagaggtaa ggcgcgcctt tttttttata
     7621 tatatatata tatatatata actgaataaa gagtatcatc tttcaaagag ctccaattcg
     7681 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     7741 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7801 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7861 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7921 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8041 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8161 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8221 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8281 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     8341 tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag
     8401 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     8461 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     8521 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     8581 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     8641 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     8701 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     8761 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     8821 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     8881 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     8941 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     9001 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     9061 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     9121 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     9181 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     9241 tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
     9301 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
     9361 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     9421 aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg
     9481 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     9541 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9601 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     9661 ga
//
