LOCUS       sequence-252234-        4306 bp    DNA     circular SYN 15-APR-2025
DEFINITION  CFP fluorescent reporter for _70.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: P_70-cfp
FEATURES             Location/Qualifiers
     source          1..4306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..168
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             468..479
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1252..1323
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1339..1366
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1897..2847)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3609..3703)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3734..4306,1..222))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3845..3864)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
       61 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      121 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      181 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
      241 tgtctcttga tcagatcatg atcccctgcg ccatcagatc cttggcggca agaaagccat
      301 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga
      361 cgtcggtgcc taatgagtga gctaacttac attaattgcg ttgcgcaata attcttgaca
      421 tttatgcttc cggctcgtat aatacgtgca attggatcct taactttaaa gaggagaaag
      481 gtaccgcatg actagcaaaa gaagcaaagg tgaagaactg ttcactggtg ttgttccaat
      541 tctggttgaa ctggatggtg atgttaatgg tcacaaattt tctgtctctg gtgagggtga
      601 aggtgatgca acctacggta aactgaccct gaaatttatt tgcactactg gtaaactgcc
      661 tgttccgtgg ccaaccctgg tcactactct gacttggggt gttcaatgct ttgctcgtta
      721 cccagatcac atgaaacagc atgacttttt caagtctgcc atgccggaag gttatgttca
      781 ggaacgtact atctttttca aagatgacgg taactacaag acccgtgctg aagtcaagtt
      841 tgaaggtgat accctggtta atcgtatcga gctgaaaggt attgatttta aagaagatgg
      901 taacattctg ggtcacaaac tggaatacaa cgctatttct gataatgtat acatcactgc
      961 tgacaaacaa aagaatggta tcaaagctaa tttcaaaatt cgtcacaaca ttgaagatgg
     1021 tagcgttcaa ctggcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1081 gctgccagac aaccattacc tgtccaccca atctcgtctg tctaaagatc cgaacgaaaa
     1141 gcgcgatcac atggtcctgc tggagtttgt aaccgctgct ggtattaccc tgggcatgga
     1201 tgaactgtat aaataatagg gatccaaact cgagtaagga tctccaggca tcaaataaaa
     1261 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1321 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctagggt
     1381 acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc
     1441 agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat
     1501 tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc
     1561 agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg
     1621 tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt
     1681 acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca
     1741 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
     1801 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
     1861 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     1921 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     1981 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     2041 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
     2101 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     2161 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     2221 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     2281 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     2341 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     2401 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     2461 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     2521 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     2581 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     2641 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     2701 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     2761 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     2821 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     2881 ataccaattg agatgggcta gtcaatgata attactagtc cttttcccgg gtgatctggg
     2941 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     3001 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     3061 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     3121 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     3181 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     3241 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     3301 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     3361 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     3421 ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc
     3481 tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg
     3541 gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcccta
     3601 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     3661 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctctcgaac
     3721 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     3781 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     3841 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     3901 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     3961 cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca
     4021 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     4081 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     4141 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     4201 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     4261 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaac
//
