LOCUS       sequence-252434-        7062 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expression of FLAG-tagged mouse CD72a in animal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7062)
  TITLE     Tsubata CD72, SmB, and U1-C plasmids
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMXCD72aFLAG-ires-YFP
FEATURES             Location/Qualifiers
     source          1..7062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..391
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             456..872
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     802..824
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     840..856
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    882..1256
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1194..1210
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2349..2372
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3078..3099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3339..3358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3498..3749
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3686..3707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3923..4392
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4522..4539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4693..5281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4773..4792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5445..6236,6237..6305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6068..6087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6306..6410)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6440..7033
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct
       61 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
      121 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa
      181 ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa
      241 atcccgatcg ttttggactc tttggtgcac cccccttaga ggagggatat gtggttctgg
      301 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg
      361 accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga
      421 ctgtgtttct gtatttgtct gaaaatatgg gcccgggcca gactgttacc actcccttaa
      481 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
      541 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
      601 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
      661 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
      721 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
      781 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
      841 tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg
      901 gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc
      961 cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc
     1021 tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg
     1081 gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac
     1141 cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga
     1201 tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgccgg
     1261 atcccagtgt ggtggtacgg gaattcatgg ctgacgctat cacgtatgca gacctgcgct
     1321 ttgtgaaagt gcccctgaag aacagcgcat ctaaccatct aggacaggac tgtgaggcct
     1381 atgaagatgg ggaactcacc tacgagaacg tgcaagtgtc tccagtccca ggagggccac
     1441 caggcttggc ttcccctgca ctagcggaca aagcaggggt cgggtcagag caaccaactg
     1501 cgacctggag ctctgtgaag tcgtctgctc tcaggcagat tccccgctgt cctacggtct
     1561 gcttgcaaaa cttcttgctt ggccttctcc tgtcctgtct gatgttaggg gtggctgtca
     1621 tctgcctggg agttcgctat ctgcaggtgt ctcagcagtt ccaggagggg accaggattt
     1681 gggaagccac caatagcagc ctgcagcagc agctcaggga gaagataagt cagctggggc
     1741 agaaggaggt ggagcttcag gagtctcaga aagagctgat ctcgagccag gacacattac
     1801 aggagaagca gaggactcac aaggacactg agcagcaact acaagcctgc caggctgaga
     1861 gagcgaagac caaggagaac ctgaaaactg aggaggagcg gaggagggac ctggaccaga
     1921 ggttgacaag cacgcgggag acactgaggc gcttgtcctc ctgttcatca gacacctgct
     1981 gtccatgcgg atggattcca tatcaggaaa ggtgctttta catctcacat accctcagaa
     2041 gtctggagga gagccaaaaa tactgcacat ctctgtcctc caaactggca gcattcgatg
     2101 aaccttctaa gtattactat gaagtttctc tgcccagcgg cttagaggag ttgctagatc
     2161 gttcgaagtc atattggata cagatgagca agaagtggag gcatgactat gactctcaaa
     2221 gccgatattg tgacaagata aaaaaatatt accagaagtg gaaaagaaca ttttctgagt
     2281 gtgcagagct tcacccctgc atttgtgagt cggaggcttt caggtttcct gatgggatcc
     2341 atctgaacga ttacaaggat gacgacgata agtgaaccgg atagcggccg cgtcgagcag
     2401 ctgaagctta aaacagctct ggggttgtac ccaccccaga ggcccacgtg gcggctagta
     2461 ctccggtatt gcggtaccct tgtacgcctg ttttatactc ccttcccgta acttagacgc
     2521 acaaaaccaa gttcaataga agggggtaca aaccagtacc accacgaaca agcacttctg
     2581 tttccccggt gatgtcgtat agactgcttg cgtggttgaa agcgacggat ccgttatccg
     2641 cttatgtact tcgagaagcc cagtaccacc tcggaatctt cgatgcgttg cgctcagcac
     2701 tcaaccccag agtgtagctt aggctgatga gtctggacat ccctcaccgg tgacggtggt
     2761 ccaggctgcg ttggcggcct acctatggct aacgccatgg gacgctagtt gtgaacaagg
     2821 tgtgaagagc ctattgagct acataagaat cctccggccc ctgaatgcgg ctaatcccaa
     2881 cctcggagca ggtggtcaca aaccagtgat tggcctgtcg taacgcgcaa gtccgtggcg
     2941 gaaccgacta ctttgggtgt ccgtgtttcc ttttatttta ttgtggctgc ttatggtgac
     3001 aatcacagat tgttatcata aagcgaattg gattgcggcc ggccgcaatg gtgaggttca
     3061 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     3241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3481 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3601 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     3661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3721 tcactctcgg catggacgag ctgtacaagt aagtcgacga taaaataaaa gattttattt
     3781 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     3841 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     3901 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     3961 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     4021 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     4081 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     4141 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     4201 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     4261 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     4321 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     4381 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
     4441 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5281 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5341 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc
     5401 cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5461 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5521 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5581 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5641 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5701 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5761 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5821 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5881 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5941 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6001 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6061 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6121 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6181 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6241 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6301 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6361 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6421 cgaaaagtgc cacctgcata atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     6481 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagaaaag ttcagatcaa
     6541 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     6601 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     6661 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     6721 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     6781 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     6841 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
     6901 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     6961 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     7021 ggggtctttc atttgggggc tcgtccggga tcgggagacc cc
//
