LOCUS       sequence-252678-       10934 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of FGFR3 E360Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR3-E360Q
FEATURES             Location/Qualifiers
     source          1..10934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5894..5918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5926..5944
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5969..5988)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5970..5988)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5994..6014)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6068..6109
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6130..6697
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6147..6699
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6291..6308)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6518..6537
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6711..7310
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6711..6730)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7207..7227
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7324..7912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7377..7397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7795..7806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7987..8220
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8339..8357)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8425..8529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8530..8598,8599..9390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8748..8767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9561..10149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10050..10069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10303..10320
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10560..10581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10596..10601,10602..10619,10620..10626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10634..10650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10639..10661
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10658..10674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10658..10674
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10724..10743
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatgggcgc ccctgcctgc
     3481 gccctcgcgc tctgcgtggc cgtggccatc gtggccggcg cctcctcgga gtccttgggg
     3541 acggagcagc gcgtcgtggg gcgagcggca gaagtcccgg gcccagagcc cggccagcag
     3601 gagcagttgg tcttcggcag cggggatgct gtggagctga gctgtccccc gcccgggggt
     3661 ggtcccatgg ggcccactgt ctgggtcaag gatggcacag ggctggtgcc ctcggagcgt
     3721 gtcctggtgg ggccccagcg gctgcaggtg ctgaatgcct cccacgagga ctccggggcc
     3781 tacagctgcc ggcagcggct cacgcagcgc gtactgtgcc acttcagtgt gcgggtgaca
     3841 gacgctccat cctcgggaga tgacgaagac ggggaggacg aggctgagga cacaggtgtg
     3901 gacacagggg ccccttactg gacacggccc gagcggatgg acaagaagct gctggccgtg
     3961 ccggccgcca acaccgtccg cttccgctgc ccagccgctg gcaaccccac tccctccatc
     4021 tcctggctga agaacggcag ggagttccgc ggcgagcacc gcattggagg catcaagctg
     4081 cggcatcagc agtggagcct ggtcatggaa agcgtggtgc cctcggaccg cggcaactac
     4141 acctgcgtcg tggagaacaa gtttggcagc atccggcaga cgtacacgct ggacgtgctg
     4201 gagcgctccc cgcaccggcc catcctgcag gcggggctgc cggccaacca gacggcggtg
     4261 ctgggcagcg acgtggagtt ccactgcaag gtgtacagtg acgcacagcc ccacatccag
     4321 tggctcaagc acgtggaggt gaacggcagc aaggtgggcc cggacggcac accctacgtt
     4381 accgtgctca agacggcggg cgctaacacc accgacaagg agctagaggt tctctccttg
     4441 cacaacgtca cctttgagga cgccggggag tacacctgcc tggcgggcaa ttctattggg
     4501 ttttctcatc actctgcgtg gctggtggtg ctgccagccc aggaggagct ggtggaggct
     4561 gacgaggcgg gcagtgtgta tgcaggcatc ctcagctacg gggtgggctt cttcctgttc
     4621 atcctggtgg tggcggctgt gacgctctgc cgcctgcgca gcccccccaa gaaaggcctg
     4681 ggctccccca ccgtgcacaa gatctcccgc ttcccgctca agcgacaggt gtccctggag
     4741 tccaacgcgt ccatgagctc caacacacca ctggtgcgca tcgcaaggct gtcctcaggg
     4801 gagggcccca cgctggccaa tgtctccgag ctcgagctgc ctgccgaccc caaatgggag
     4861 ctgtctcggg cccggctgac cctgggcaag ccccttgggg agggctgctt cggccaggtg
     4921 gtcatggcgg aggccatcgg cattgacaag gaccgggccg ccaagcctgt caccgtagcc
     4981 gtgaagatgc tgaaagacga tgccactgac aaggacctgt cggacctggt gtctgagatg
     5041 gagatgatga agatgatcgg gaaacacaaa aacatcatca acctgctggg cgcctgcacg
     5101 cagggcgggc ccctgtacgt gctggtggag tacgcggcca agggtaacct gcgggagttt
     5161 ctgcgggcgc ggcggccccc gggcctggac tactccttcg acacctgcaa gccgcccgag
     5221 gagcagctca ccttcaagga cctggtgtcc tgtgcctacc aggtggcccg gggcatggag
     5281 tacttggcct cccagaagtg catccacagg gacctggctg cccgcaatgt gctggtgacc
     5341 gaggacaacg tgatgaagat cgcagacttc gggctggccc gggacgtgca caacctcgac
     5401 tactacaaga agacaaccaa cggccggctg cccgtgaagt ggatggcgcc tgaggccttg
     5461 tttgaccgag tctacactca ccagagtgac gtctggtcct ttggggtcct gctctgggag
     5521 atcttcacgc tggggggctc cccgtacccc ggcatccctg tggaggagct cttcaagctg
     5581 ctgaaggagg gccaccgcat ggacaagccc gccaactgca cacacgacct gtacatgatc
     5641 atgcgggagt gctggcatgc cgcgccctcc cagaggccca ccttcaagca gctggtggag
     5701 gacctggacc gtgtccttac cgtgacgtcc accgacgagt acctggacct gtcggcgcct
     5761 ttcgagcagt actccccggg tggccaggac acccccagct ccagctcctc aggggacgac
     5821 tccgtgtttg cccacgacct gctgcccccg gccccaccca gcagtggggg ctcgcggacg
     5881 tagggcctca tgggcccagc tttcttgtac aaagtggtga tatccaatta accctcacta
     5941 aaggagagta ttagtagtta ttgtaagtcc ctatagtgag tcgtattacc acccaacttt
     6001 tctatacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     6061 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta
     6121 gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     6181 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     6241 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     6301 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     6361 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     6421 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     6481 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     6541 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     6601 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     6661 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat atgaccgagt
     6721 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     6781 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     6841 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     6901 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     6961 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7021 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7081 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7141 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7201 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7261 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctaa atcgatagat
     7321 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7381 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7441 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7501 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7561 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7621 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7681 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7741 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7801 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7861 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     7921 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7981 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     8041 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8101 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8161 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8221 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8281 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     8341 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8401 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8461 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8521 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8581 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8641 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8701 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8761 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8821 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8881 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8941 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9001 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9061 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9121 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9181 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9241 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9301 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9361 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9421 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9481 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9541 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9601 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9661 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9721 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9781 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9841 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9901 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9961 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10021 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10081 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10141 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10201 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10261 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10321 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10381 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    10441 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
    10501 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10561 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10621 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
    10681 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10741 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10801 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10861 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10921 acaaacttag tagt
//
