LOCUS       sequence-252679-       10933 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of FGFR3 R124W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR3-R124W
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5894..5918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5926..5944
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5969..5988)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5970..5988)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5994..6014)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6068..6109
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6130..6696
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6146..6698
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6290..6307)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6517..6536
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6710..7309
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6710..6729)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7206..7226
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7323..7911
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7376..7396)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7794..7805)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7986..8219
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8338..8356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8424..8528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8529..8597,8598..9389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8747..8766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9560..10148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10049..10068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10302..10319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10559..10580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10595..10600,10601..10618,10619..10625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10633..10649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10638..10660
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10657..10673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10657..10673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10723..10742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatgggcgc ccctgcctgc
     3481 gccctcgcgc tctgcgtggc cgtggccatc gtggccggcg cctcctcgga gtccttgggg
     3541 acggagcagc gcgtcgtggg gcgagcggca gaagtcccgg gcccagagcc cggccagcag
     3601 gagcagttgg tcttcggcag cggggatgct gtggagctga gctgtccccc gcccgggggt
     3661 ggtcccatgg ggcccactgt ctgggtcaag gatggcacag ggctggtgcc ctcggagcgt
     3721 gtcctggtgg ggccccagcg gctgcaggtg ctgaatgcct cccacgagga ctccggggcc
     3781 tacagctgcc ggcagcggct cacgcagcgc gtactgtgcc acttcagtgt gtgggtgaca
     3841 gacgctccat cctcgggaga tgacgaagac ggggaggacg aggctgagga cacaggtgtg
     3901 gacacagggg ccccttactg gacacggccc gagcggatgg acaagaagct gctggccgtg
     3961 ccggccgcca acaccgtccg cttccgctgc ccagccgctg gcaaccccac tccctccatc
     4021 tcctggctga agaacggcag ggagttccgc ggcgagcacc gcattggagg catcaagctg
     4081 cggcatcagc agtggagcct ggtcatggaa agcgtggtgc cctcggaccg cggcaactac
     4141 acctgcgtcg tggagaacaa gtttggcagc atccggcaga cgtacacgct ggacgtgctg
     4201 gagcgctccc cgcaccggcc catcctgcag gcggggctgc cggccaacca gacggcggtg
     4261 ctgggcagcg acgtggagtt ccactgcaag gtgtacagtg acgcacagcc ccacatccag
     4321 tggctcaagc acgtggaggt gaacggcagc aaggtgggcc cggacggcac accctacgtt
     4381 accgtgctca agacggcggg cgctaacacc accgacaagg agctagaggt tctctccttg
     4441 cacaacgtca cctttgagga cgccggggag tacacctgcc tggcgggcaa ttctattggg
     4501 ttttctcatc actctgcgtg gctggtggtg ctgccagccg aggaggagct ggtggaggct
     4561 gacgaggcgg gcagtgtgta tgcaggcatc ctcagctacg gggtgggctt cttcctgttc
     4621 atcctggtgg tggcggctgt gacgctctgc cgcctgcgca gcccccccaa gaaaggcctg
     4681 ggctccccca ccgtgcacaa gatctcccgc ttcccgctca agcgacaggt gtccctggag
     4741 tccaacgcgt ccatgagctc caacacacca ctggtgcgca tcgcaaggct gtcctcaggg
     4801 gagggcccca cgctggccaa tgtctccgag ctcgagctgc ctgccgaccc caaatgggag
     4861 ctgtctcggg cccggctgac cctgggcaag ccccttgggg agggctgctt cggccaggtg
     4921 gtcatggcgg aggccatcgg cattgacaag gaccgggccg ccaagcctgt caccgtagcc
     4981 gtgaagatgc tgaaagacga tgccactgac aaggacctgt cggacctggt gtctgagatg
     5041 gagatgatga agatgatcgg gaaacacaaa aacatcatca acctgctggg cgcctgcacg
     5101 cagggcgggc ccctgtacgt gctggtggag tacgcggcca agggtaacct gcgggagttt
     5161 ctgcgggcgc ggcggccccc gggcctggac tactccttcg acacctgcaa gccgcccgag
     5221 gagcagctca ccttcaagga cctggtgtcc tgtgcctacc aggtggcccg gggcatggag
     5281 tacttggcct cccagaagtg catccacagg gacctggctg cccgcaatgt gctggtgacc
     5341 gaggacaacg tgatgaagat cgcagacttc gggctggccc gggacgtgca caacctcgac
     5401 tactacaaga agacaaccaa cggccggctg cccgtgaagt ggatggcgcc tgaggccttg
     5461 tttgaccgag tctacactca ccagagtgac gtctggtcct ttggggtcct gctctgggag
     5521 atcttcacgc tggggggctc cccgtacccc ggcatccctg tggaggagct cttcaagctg
     5581 ctgaaggagg gccaccgcat ggacaagccc gccaactgca cacacgacct gtacatgatc
     5641 atgcgggagt gctggcatgc cgcgccctcc cagaggccca ccttcaagca gctggtggag
     5701 gacctggacc gtgtccttac cgtgacgtcc accgacgagt acctggacct gtcggcgcct
     5761 ttcgagcagt actccccggg tggccaggac acccccagct ccagctcctc aggggacgac
     5821 tccgtgtttg cccacgacct gctgcccccg gccccaccca gcagtggggg ctcgcggacg
     5881 tagggcctca tgggcccagc tttcttgtac aaagtggtga tatccaatta accctcacta
     5941 aagggtaaga ttagaagtta atgaagaacc ctatagtgag tcgtattacc acccaacttt
     6001 tctatacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     6061 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta
     6121 gtaatgagat ccctcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6181 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6241 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6301 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6361 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6421 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6481 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6541 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6601 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6661 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata tgaccgagta
     6721 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     6781 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6841 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6901 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6961 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7021 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7081 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7141 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     7201 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7261 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc
     7321 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7381 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7441 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7501 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7561 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7621 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7681 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7741 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7801 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7861 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     7921 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7981 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8041 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8101 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8161 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8221 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8281 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8341 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8761 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9241 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10321 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10381 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10441 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10501 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10561 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10621 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    10681 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10741 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10801 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    10861 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10921 caaacttagt agt
//
